Adsorption of proteins on gold nanoparticles: One or more layers?

Sotnikov, Dmitriy V.; Berlina, Anna N.; Иванов, В. С.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1016/j.colsurfb.2018.10.025

Cited by 72 publications

(50 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We note that the results obtained with PS‐COOH NPs ( R H ≈ 35 nm) are in close agreement with those obtained with much smaller carboxyl‐functionalized NPs ( R H ≈ 5 nm) . Thus, we confirmed the presence of a soft monolayer corona also for larger NPs, in contrast to other studies …”

Section: Resultssupporting

confidence: 91%

“…There are many studies in the literature reporting protein multilayer formation on the surface of nanomaterials . However, in our own work with small NPs ( R H ≈ 5–10 nm), in which we directly determined the NP size increase due to protein adsorption as a function of the concentration of protein solutions and blood serum, we ubiquitously observed only thin coronae compatible with protein monolayers.…”

Section: Discussionmentioning

confidence: 70%

“…So why are there so many studies in the literature reporting protein multilayer formation on the surface of nanomaterials? In some instances, the thickness of the protein corona is determined in an indirect fashion, and we may speculate about the reliability of the method. However, as we have shown here, even direct sizing methods such as DLS may yield large apparent size increases due to the presence of NP agglomerates, which can erroneously be interpreted as multilayers of adsorbed proteins on individual NPs.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Wang

Nienhaus

et al. 2019

Small

View full text Add to dashboard Cite

Nanoparticle (NP) interactions with cells and organisms are mediated by a biomolecular adsorption layer, the so‐called “protein corona.” An in‐depth understanding of the corona is a prerequisite to successful and safe application of NPs in biology and medicine. In this work, earlier in situ investigations on small NPs are extended to large polystyrene (PS) NPs of up to 100 nm diameter, using human transferrin (Tf) and human serum albumin (HSA) as model proteins. Direct NP sizing experiments reveal a reversibly bound monolayer protein shell (under saturating conditions) on hydrophilic, carboxyl‐functionalized (PS‐COOH) NPs, as was earlier observed for much smaller NPs. In contrast, protein binding on hydrophobic, sulfated (PS‐OSO3H) NPs in solvent of low ionic strength is completely irreversible; nevertheless, the thickness of the observed protein corona again corresponds to a protein monolayer. Under conditions of reduced charge repulsion (higher ionic strength), the NPs are colloidally unstable and form large clusters below a certain protein–NP stoichiometric ratio, indicating that the adsorbed proteins induce NP agglomeration. This comprehensive characterization of the persistent protein corona on PS‐OSO3H NPs by nanoparticle sizing and quantitative fluorescence microscopy/nanoscopy reveals mechanistic aspects of molecular interactions occurring during exposure of NPs to biofluids.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Wang

Nienhaus

et al. 2019

Small

View full text Add to dashboard Cite

show abstract

“…For example, Sotnikov et al performed a comparative theoretical and experimental study for two alternate LFA formats applied to the serodiagnosis of human pulmonary tuberculosis. 72 Detection of low-affinity antibodies in human sera samples was 6-fold more sensitive for the alternate format predicted by their theoretical model. Capture reagents.…”

Section: Reaction/transportmentioning

confidence: 96%

Sensitivity enhancement in lateral flow assays: a systems perspective

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Conjugation was performed according to the procedure described in [23], with minor modifications. A solution of gold nanoparticles (optical density at 520 nm: OD 520 = 1) was adjusted with potassium carbonate to pH 8.5-9.0 (basing the results of our previous studies [34,35]), after which the Cys-A/G protein (ProsPec, Israel) was added to this solution being diluted to the concentration of 15 µg/mL. The mixture was incubated for 1 h at room temperature; then, a 10% BSA solution (Boval Biosolutions, USA) was added and the mixture kept for another 10 min with vigorous stirring.…”

Section: Obtaining Conjugates Of Gold Nanoparticles With Cysteine-a/gmentioning

confidence: 99%

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

et al. 2019

Self Cite

View full text Add to dashboard Cite

An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.

show abstract

Adsorption of proteins on gold nanoparticles: One or more layers?

Cited by 72 publications

References 47 publications

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Sensitivity enhancement in lateral flow assays: a systems perspective

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Contact Info

Product

Resources

About