Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes

Prlainović, Nevena Ž.; Bezbradica, Dejan; Knežević‐Jugović, Zorica; Stevanović, Sanja; Ivić, Milka L. Avramov; Uskoković, Petar S.; Mijin, Dušan Ž.

doi:10.1016/j.jiec.2012.08.012

Cited by 58 publications

(32 citation statements)

References 40 publications

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“…However, the intensity of ÀC D O is reduced, expanded, and shifted to a lower frequency of 1121 cm ¡1 , 1110 cm ¡1 in the CRL-MWCNTs. Hence, the investigation agreed with previous findings by Prlainovic et al [32] Thermogravimetric analysis (TGA) The thermal decomposition of both synthesizedMWCNTs and acid-functionalized MWCNTs were analysed by a thermogravimetric analyser and the results are illustrated in Figure 2. The thermogram revealed that a large loss of mass of approximately 62% was observed for the MWCNTs, between 550 C and 700 C (Figure 2(a)).…”

Section: Characterization Of Crl-mwcntssupporting

confidence: 89%

“…It can be concluded that the immobilization process considerably altered the structure of the CRL-MWCNTs, following adsorption on to the highly thermostable carbon nanotubes supports. The F-MWCNTs allowed for a robust setting for the CRL enzyme linkage [32,44] and improved the hydrophobicity of the microenvironment. [45,46] Nonetheless, the concentration of the formed ester declined at temperatures exceeding 50 C, which was probably caused by the partial inactivation of the CRLMWCNTs.…”

Section: Rsm Experiments and Model Fittingmentioning

confidence: 99%

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Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Marzuki

Huyop

Aboul‐Enein

et al. 2015

Biotechnology & Biotechnological Equipment

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Esters, such as methyl oleate, are functionally important compounds in many industrial sectors, mainly as components for manufacturing of emulsifiers, detergents, intermediate stabilizers and wetting agents. Acid functionalization of multiwalled carbon nanotubes (F-MWCNTs) by using a mixture of HNO 3 and H 2 SO 4 (1:3, v:v) was employed as the matrix for the adsorption of Candida rugosa lipase (CRL) as nanobioconjugates (CRL-MWCNTs) for the production of methyl oleate. Structural information of the developed CRL-MWCNTs was confirmed using Fourier Transform Infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy, which revealed a successful attachment of CRL onto the F-MWCNTs. Process parameters (reaction time, temperature and alcohol:acid molar ratio) were optimized for high percent conversion of methyl oleate. Structural analysis established that CRL was successfully attached to the surface of the F-MWCNTs. Under the optimized conditions, which were 13.87 h, 51 C, molar ratio oleic acid: methanol (1:3.80), a high ester yield of 90.90% was attained. Also, under conditions of the shortest reaction time of 6 h, 47.07 C and acid:methanol ratio of 1:2.54, the CRL-MWCNTs catalysed a 74.51% ester yield. Hence, we established that response surface methodology (RSM) can be a practical technique for the prediction of the conditions that favour the high yield production of methyl oleate. Under optimized conditions, the CRL-MWCNTs nanobioconjugates are potentially good and economical biocatalysts for the production of methyl oleate, as well as other types of commercially important fatty-acid methyl esters.

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Section: Characterization Of Crl-mwcntssupporting

confidence: 89%

Section: Rsm Experiments and Model Fittingmentioning

confidence: 99%

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Marzuki

Huyop

Aboul‐Enein

et al. 2015

Biotechnology & Biotechnological Equipment

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show abstract

“…2b) may be attributable to vibrations of C-O carboxylate moiety. Consistent with the findings reported by previous researchers [33], FT-IR results further revealed that following immobilization, the vibrations of C-O of the carboxylate moiety were reduced, expanded, and moved to a lower frequency range (1121-1110 cm −1 ), indicating that the CRL was successfully immobilized onto the outer surface of the acid-functionalized MWCNTs.…”

Section: Characterization Of the Crl-mwcnt Biocatalystssupporting

confidence: 90%

“…No further loss in mass was observed after 700°C because at this point, the residue consisted essentially of thermally stable carbon [23]. Since enzyme molecules tend to decompose at lower temperatures than other chemical groups [33] introduced onto the MWCNTs in this paper, the loss of mass (19 %) for CRL-MWCNTs that occurred at lower temperatures (150-450°C) (Fig. 3c) may be attributed to the decomposition of CRL (Fig.…”

Section: Characterization Of the Crl-mwcnt Biocatalystsmentioning

confidence: 79%

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

Marzuki

Mahat

Huyop

et al. 2015

Appl Biochem Biotechnol

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The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via environmentally favorable processes is of concern. In this context, an alternative technique utilizing Candida rugosa lipase (CRL) physically adsorbed on multi-walled carbon nanotubes (MWCNTs) has been suggested. In this study, the acid-functionalized MWCNTs prepared using a mixture of HNO3 and H2SO4 (1:3 v/v) was used as support for immobilizing CRL onto MWCNTs (CRL-MWCNTs) as biocatalysts. Enzymatic esterification was performed and the efficiency of CRL-MWCNTs was evaluated against the free CRL under varying conditions, viz. temperature, molar ratio of acid/alcohol, solvent log P, and enzyme loading. The CRL-MWCNTs resulted in 30-110 % improvement in the production of methyl oleate over the free CRL. The CRL-MWCNTs attained its highest yield (84.17 %) at 50 °C, molar ratio of acid/alcohol of 1:3, 3 mg/mL of enzyme loading, and iso-octane (log P 4.5) as solvent. Consequently, physical adsorption of CRL onto acid-functionalized MWCNTs has improved the activity and stability of CRL and hence provides an environmentally friendly means for the production of methyl oleate.

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“…This simple physical adsorption can also be applied to un-modified multi-walled CNTs surfaces, such as lipase adsorption. Prlainovic et al [60] found that the time required for complete enzyme adsorption depended on the ionic strength of the medium. Based on their observation, the ionic strength enhanced hydrophobic interactions which led to successful enzyme immobilization.…”

Section: Carbon Nanocarriersmentioning

confidence: 99%

Nanobiocatalyst advancements and bioprocessing applications

Misson

Zhang

Jin

2015

J. R. Soc. Interface.

210

108

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The nanobiocatalyst (NBC) is an emerging innovation that synergistically integrates advanced nanotechnology with biotechnology and promises exciting advantages for improving enzyme activity, stability, capability and engineering performances in bioprocessing applications. NBCs are fabricated by immobilizing enzymes with functional nanomaterials as enzyme carriers or containers. In this paper, we review the recent developments of novel nanocarriers/nanocontainers with advanced hierarchical porous structures for retaining enzymes, such as nanofibres (NFs), mesoporous nanocarriers and nanocages. Strategies for immobilizing enzymes onto nanocarriers made from polymers, silicas, carbons and metals by physical adsorption, covalent binding, cross-linking or specific ligand spacers are discussed. The resulting NBCs are critically evaluated in terms of their bioprocessing performances. Excellent performances are demonstrated through enhanced NBC catalytic activity and stability due to conformational changes upon immobilization and localized nanoenvironments, and NBC reutilization by assembling magnetic nanoparticles into NBCs to defray the high operational costs associated with enzyme production and nanocarrier synthesis. We also highlight several challenges associated with the NBC-driven bioprocess applications, including the maturation of large-scale nanocarrier synthesis, design and development of bioreactors to accommodate NBCs, and long-term operations of NBCs. We suggest these challenges are to be addressed through joint collaboration of chemists, engineers and material scientists. Finally, we have demonstrated the great potential of NBCs in manufacturing bioprocesses in the near future through successful laboratory trials of NBCs in carbohydrate hydrolysis, biofuel production and biotransformation.

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Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes

Cited by 58 publications

References 40 publications

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Modelling and optimization ofCandida rugosananobioconjugates catalysed synthesis of methyl oleate by response surface methodology

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

Nanobiocatalyst advancements and bioprocessing applications

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