Adsorption of bovine serum albumin on gold nanoprisms: interaction and effect of NIR irradiation on protein corona

Célis, Freddy; Garrido, C.; Campos, Marcelo; Guzmán, Fanny; Kogan, Marcelo J.; Araya, Eyleen

doi:10.1039/d0tb01246g

Cited by 17 publications

(19 citation statements)

References 113 publications

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“…Gold nanoparticles (AuNP) also show promising characteristics in drug delivery systems, as they present high solubility, high stability in living systems and improved kinetics for drug distribution [ 30 , 31 , 32 , 33 ]. AuNPs can be conjugated to inclusion complexes (IC) of CDs if the guests have functional groups, such as thiols or amines [ 34 , 35 ]. NSs associated with AuNPs might promote the release of the included drugs by means of irradiation, due to the plasmon effect of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin Nanosponges Inclusion Compounds Associated with Gold Nanoparticles for Potential Application in the Photothermal Release of Melphalan and Cytoxan

Salazar

Yutronic

Kogan

et al. 2021

IJMS

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This article describes the synthesis and characterization of β-cyclodextrin-based nano-sponges (NS) inclusion compounds (IC) with the anti-tumor drugs melphalan (MPH) and cytoxan (CYT), and the addition of gold nanoparticles (AuNPs) onto both systems, for the potential release of the drugs by means of laser irradiation. The NS-MPH and NS-CYT inclusion compounds were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRPD), energy dispersive spectroscopy (EDS), thermogravimetric analysis (TGA), UV–Vis, and proton nuclear magnetic resonance (1H-NMR). Thus, the inclusion of MPH and CYT inside the cavities of NSs was confirmed. The association of AuNPs with the ICs was confirmed by SEM, EDS, TEM, and UV–Vis. Drug release studies using NSs synthesized with different molar ratios of β-cyclodextrin and diphenylcarbonate (1:4 and 1:8) demonstrated that the ability of NSs to entrap and release the drug molecules depends on the crosslinking between the cyclodextrin monomers. Finally, irradiation assays using a continuous laser of 532 nm showed that photothermal drug release of both MPH and CYT from the cavities of NSs via plasmonic heating of AuNPs is possible.

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Section: Introductionmentioning

confidence: 99%

Cyclodextrin Nanosponges Inclusion Compounds Associated with Gold Nanoparticles for Potential Application in the Photothermal Release of Melphalan and Cytoxan

Salazar

Yutronic

Kogan

et al. 2021

IJMS

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“…AuNPrs were obtained by Na 2 S 2 O 3 reduction of HAuCl 4 , as previously reported [ 28 , 36 ]. A 2 mM HAuCl 4 solution was first reduced by 0.6 mM Na 2 S 2 O 3 and allowed to rest for 9 min.…”

Section: Methodsmentioning

confidence: 99%

“…The purple solution containing the AuNPs was centrifuged and resuspended in Milli-Q water. PEG functionalization was achieved at pH = 12 by adding 15 µL of a 2.7 mM HS-PEG5000-COOH solution and allowing conjugation under a magnetic stirrer for 3 h. Finally, AuNPrs were separated from smaller undesired AuNPs using a successive differential centrifugation procedure, as reported [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

“…BSA-r 8 or BSA capping of the AuNPs was achieved by incubation, as previously reported [ 36 ]. Briefly, to 1 mL of AuNRs or AuNPrs at a 1 nM concentration, a solution of BSA or BSA-r 8 was added (final concentration of BSA = 1 mg/mL in 0.1× PBS), incubating for 2 h at 4 °C in low-binding 1.5 mL centrifuge tubes (Eppendorf ® , Hamburg, Germany), and the samples were centrifuged at 10,000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…In order to avoid possible cytotoxic effects from our nanocarriers, we incorporated a protein coating on both AuNPs to increase their biocompatibility and stability [ 6 , 29 , 33 , 34 , 35 , 36 ]. BSA shares 76% sequence identity with human serum albumin (HSA) [ 37 ] and is well tolerated by humans [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

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Oligoarginine Peptide Conjugated to BSA Improves Cell Penetration of Gold Nanorods and Nanoprisms for Biomedical Applications

et al. 2021

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Gold nanoparticles (AuNPs) have been shown to be outstanding tools for drug delivery and biomedical applications, mainly owing to their colloidal stability, surface chemistry, and photothermal properties. The biocompatibility and stability of nanoparticles can be improved by capping the nanoparticles with endogenous proteins, such as albumin. Notably, protein coating of nanoparticles can interfere with and decrease their cell penetration. Therefore, in the present study, we functionalized albumin with the r8 peptide (All-D, octaarginine) and used it for coating NIR-plasmonic anisotropic gold nanoparticles. Gold nanoprisms (AuNPrs) and gold nanorods (AuNRs) were coated with bovine serum albumin (BSA) previously functionalized using a cell penetrating peptide (CPP) with the r8 sequence (BSA-r8). The effect of the coated and r8-functionalized AuNPs on HeLa cell viability was assessed by the MTS assay, showing a low effect on cell viability after BSA coating. Moreover, the internalization of the nanostructures into HeLa cells was assessed by confocal microscopy and transmission electron microscopy (TEM). As a result, both nanoconstructs showed an improved internalization level after being capped with BSA-r8, in contrast to the BSA-functionalized control, suggesting the predominant role of CPP functionalization in cell internalization. Thus, our results validate both novel nanoconstructs as potential candidates to be coated by endogenous proteins and functionalized with a CPP to optimize cell internalization. In a further approach, coating AuNPs with CPP-functionalized BSA can broaden the possibilities for biomedical applications by combining their optical properties, biocompatibility, and cell-penetration abilities.

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Mesoporous Silica Nanoparticles Functionalized with Amino Groups for Biomedical Applications

et al. 2021

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The synthesis and characterization of amino‐functionalized mesoporous silica nanoparticles are presented following two different synthetic methods: co‐condensation and post‐synthesis grafting of 3‐aminopropyltriethoxysilane. The amino groups’ distribution on the mesoporous silica nanoparticles was evaluated considering the aggregation state of a grafted photosensitizer (Verteporfin) by using spectroscopic techniques. The homogeneous distribution of amino groups within the silica network is a key factor to avoid aggregation during further organic functionalization and to optimize the performance of functionalized silica nanoparticles in biomedical applications. In addition, the formation of a protein corona on the external surface of both bare and amino‐functionalized mesoporous silica was also investigated by adsorbing Bovine Serum Albumin (BSA) as a model protein. The adsorption of BSA was found to be favorable, reducing the aggregation phenomena for both bare and amino‐modified nanoparticles. Nevertheless, the dispersant effect of BSA was much more evident in the case of amino‐modified nanoparticles, which reached monodispersion after adsorption of the protein, thus suggesting that amino‐modified nanoparticles can benefit from protein corona formation for preventing severe aggregation in biological media.

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Adsorption of bovine serum albumin on gold nanoprisms: interaction and effect of NIR irradiation on protein corona

Abstract: Because of their photothermal properties, gold nanoparticles (AuNPs) have gained attention for their use in drug delivery and therapeutic applications. In this sense, it is interesting to consider their interactions...

Cited by 17 publications

References 113 publications

Cyclodextrin Nanosponges Inclusion Compounds Associated with Gold Nanoparticles for Potential Application in the Photothermal Release of Melphalan and Cytoxan

Cyclodextrin Nanosponges Inclusion Compounds Associated with Gold Nanoparticles for Potential Application in the Photothermal Release of Melphalan and Cytoxan

Oligoarginine Peptide Conjugated to BSA Improves Cell Penetration of Gold Nanorods and Nanoprisms for Biomedical Applications

Mesoporous Silica Nanoparticles Functionalized with Amino Groups for Biomedical Applications

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