Adropin stimulates proliferation but suppresses differentiation in rat primary brown preadipocytes

Jasaszwili, Mariami; Wojciechowicz, Tatiana; Strowski, Mathias Z.; Nowak, Krzysztof W.; Skrzypski, Marek

doi:10.1016/j.abb.2020.108536

Cited by 11 publications

(13 citation statements)

References 49 publications

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“…Firstly, we found that acting on differentiated rat adipocytes and 3T3-L1 cells, adropin slightly promotes the secretion of glycerol and FFA, the hallmarks of lipolysis [22]. In our previous study, we found that adropin is able to downregulate the differentiation of white and brown rodent preadipocytes into mature fat cells [17,23]. Importantly, the suppressive effect on the differentiation of both types of preadipocytes was accompanied by lower intracellular lipid content, suggesting that adropin may be involved in controlling lipid metabolism.…”

Section: Discussionmentioning

confidence: 94%

Adropin Slightly Modulates Lipolysis, Lipogenesis and Expression of Adipokines but Not Glucose Uptake in Rodent Adipocytes

Jasaszwili

Pruszyńska‐Oszmałek

Wojciechowicz

et al. 2021

Genes

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Adropin is a peptide hormone which modulates energy homeostasis and metabolism. In animals with diet-induced obesity, adropin attenuates adiposity and improves lipid and glucose homeostasis. Adropin promotes the proliferation of rodent white preadipocytes and suppresses their differentiation into adipocytes. By contrast, the effects of adropin on mature white adipocytes are unknown. Therefore, we aimed to evaluate the effects of adropin on lipolysis, lipogenesis and glucose uptake in white rodent adipocytes. We assessed the effects of adropin on the mRNA expression of adiponectin, resistin and visfatin. White preadipocytes were isolated from male Wistar rats. Differentiated 3T3-L1 cells were used as a surrogate model of white adipocytes. Lipolysis was measured by the evaluation of glycerol and free fatty acid secretion using colorimetric kits. The effects of adropin on lipogenesis and glucose uptake were measured using radioactive-labelled glucose. The expression of adipokine mRNA was studied using real-time PCR. Our results show that adropin slightly promotes lipolysis in rat adipocytes and 3T3-L1 cells. Adropin suppresses lipogenesis in rat adipocytes without influencing glucose uptake. In addition, adropin stimulates adiponectin mRNA expression and suppresses the expression of resistin and visfatin. These results indicate that adropin may be involved in controlling lipid metabolism and adipokine expression in white rodent adipocytes.

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Section: Discussionmentioning

confidence: 94%

Adropin Slightly Modulates Lipolysis, Lipogenesis and Expression of Adipokines but Not Glucose Uptake in Rodent Adipocytes

Jasaszwili

Pruszyńska‐Oszmałek

Wojciechowicz

et al. 2021

Genes

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“…In brief, rat brown preadipocytes were treated with differentiation medium with or without NPB (100 n m ) for 24 h. Thereafter, cells were exposed to adrenaline (1 µ m ) for 30 min. Extracellular oxygen consumption was measured using Extracellular Oxygen Consumption Assay (Abcam) as we previously descripted [14].…”

Section: Methodsmentioning

confidence: 99%

“…The isolation of primary brown preadipocytes was carried out as described previously [13,14]. Rat brown preadipocytes were isolated as stromal-vascular fraction of interscapular fat pad cut from male Wistar rats (100-120 g).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes

et al. 2021

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Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the role of NPB in controlling brown adipogenesis in rat brown preadipocytes. We first detected the expression of NPBWR1 and NPB on mRNA and protein level in brown preadipocytes and observed that NPB increased viability and proliferation of preadipocytes. Moreover, NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and suppressed the expression of antiadipogenic preadipocyte factor 1 (Pref1) during the differentiation process. Altogether, this led to an increase in intracellular lipid accumulation during preadipocyte differentiation, coupled with an increase in adrenaline‐induced oxygen consumption mediated by NPB. Furthermore, Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. In summary, we conclude that NPB promotes proliferation and differentiation of rat brown preadipocytes via p38‐dependent mechanism and plays an important role in controlling brown adipose tissue formation.

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“…In addition, we found that, in white rat primary preadipocytes or 3T3-L1 cells, adropin suppresses the expression of Ppar γ , Fabp4 , and C / ebp β at the mRNA level and attenuates lipid accumulation [ 22 ]. Similarly, we found that adropin downregulates intracellular lipid levels and the expression of Ucp1 , Ppar γ , and Pgc-1 α mRNA during the differentiation of rat brown preadipocytes [ 23 ]. Keeping in mind that the abovementioned genes are involved in white and brown adipogenesis [ 25 ], these results suggest that adropin suppresses the differentiation of white and brown preadipocytes into mature fat cells.…”

Section: Adropinmentioning

confidence: 99%

“…These data suggest that adropin may be involved in controlling adipose tissue functions. Recently, we showed that the adropin precursor gene, Enho , and its receptor, Gpr19 , are expressed in white and brown undifferentiated and differentiated rat preadipocytes [ 22 , 23 ]. Moreover, ENHO mRNA was detected in the adipose tissue in baboons ( Papio anubis ) [ 10 ].…”

Section: Adropinmentioning

confidence: 99%

The Role of Peptide Hormones Discovered in the 21st Century in the Regulation of Adipose Tissue Functions

Kołodziejski

Pruszyńska‐Oszmałek

Wojciechowicz

et al. 2021

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Peptide hormones play a prominent role in controlling energy homeostasis and metabolism. They have been implicated in controlling appetite, the function of the gastrointestinal and cardiovascular systems, energy expenditure, and reproduction. Furthermore, there is growing evidence indicating that peptide hormones and their receptors contribute to energy homeostasis regulation by interacting with white and brown adipose tissue. In this article, we review and discuss the literature addressing the role of selected peptide hormones discovered in the 21st century (adropin, apelin, elabela, irisin, kisspeptin, MOTS-c, phoenixin, spexin, and neuropeptides B and W) in controlling white and brown adipogenesis. Furthermore, we elaborate how these hormones control adipose tissue functions in vitro and in vivo.

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Adropin stimulates proliferation but suppresses differentiation in rat primary brown preadipocytes

Cited by 11 publications

References 49 publications

Adropin Slightly Modulates Lipolysis, Lipogenesis and Expression of Adipokines but Not Glucose Uptake in Rodent Adipocytes

Adropin Slightly Modulates Lipolysis, Lipogenesis and Expression of Adipokines but Not Glucose Uptake in Rodent Adipocytes

Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes

The Role of Peptide Hormones Discovered in the 21st Century in the Regulation of Adipose Tissue Functions

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