Adoptive transfer of immunity from mice immunized with ribosomes or live yeast cells of Histoplasma capsulatum

Tewari, Ram P.; Sharma, Dian; Solotorovsky, Morris; LaFemina, Robert L.; Balint, Joseph P.

doi:10.1128/iai.15.3.789-795.1977

Cited by 47 publications

(15 citation statements)

References 17 publications

(22 reference statements)

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“…It is generally held that a critical host defense mechanism activated in response to infection by Histoplasma capsulatum is the so-called cellmediated immune response. Support for this concept is provided by several experimental studies: first, by the demonstration that lymphocytes from mice immunized by sublethal infection with H. capsulatum can mediate suppression of intracellular growth of the fungus in normal mouse macrophages (17); second, by demonstration of increased susceptibility to Histoplasma infection in congenitally athymic mice (38) or in conventional mice after treatment with antilymphocyte serum and cytotoxic agents (1,8,12); and third, by evidence for transfer of adoptive immunity against Histoplasma by spleen or peritoneal cells from immunized donors (36).…”

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confidence: 99%

Immunoregulatory Responses in Experimental Disseminated Histoplasmosis: Lymphoid Organ Histopathology and Serological Studies

Artz

Bullock

1979

Infect Immun

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To study immunoregulatory mechanisms in systemic histoplasmosis, a highly reproducible model of infection was established in C3H/Anf mice. Intravenous inoculation of 6- to 8-week-old mice with from 5 × 10 5 to 10 × 10 5 cells of yeast-phase Histoplasma capsulatum strain G-217B produced disseminated infection that resolved over an 8-week period without therapeutic intervention. Serological studies demonstrated complement-fixing antibody production to yeast- and mycelial-phase antigens of H. capsulatum . Complement-fixing antibody to the former was detected at week 1, and it peaked at week 3 and declined thereafter. Complement-fixing antibody to mycelial-phase antigen(s) appeared later (week 3) and did not peak until week 18. Grossly, the spleens of infected mice were enlarged from three to four times normal size during peak infection, whereas the thymuses were markedly involuted. Conversely, at week 8 the average spleen size was considerably smaller and the thymic mass was increased relative to the mass at week 3. Histopathologically, the paracortical regions of lymph nodes and the white pulp (periarteriolar lymphocyte sheaths) and marginal zones of the spleen were heavily infiltrated by granulomata at week 1. By week 8, the infiltrates in these areas had largely resolved. Thymocytes were severely depleted from the cortical lobules of the thymus at week 1; however, thymic cellularity was restored by week 8. These reciprocal changes in cellularity of the thymus and spleen during active infection may be of importance with reference to the disturbances of immunoregulation that we have observed in Histoplasma -infected mice.

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confidence: 99%

Immunoregulatory Responses in Experimental Disseminated Histoplasmosis: Lymphoid Organ Histopathology and Serological Studies

Artz

Bullock

1979

Infect Immun

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“…There is a strong clinical association between AIDS and invasive histoplasmosis (62), cryptococcosis (63) and coccidiodomycosis (64,65), suggesting that cell-mediated immunity is critical. The importance of cell-mediated immunity has been demonstrated further by studies where the transfer of immune lymphocytes to naive syngeneic recipients has afforded protection against histoplasmosis (66), blastomycosis (67) and coccidiodomycosis (68). It has been demonstrated that both CD4 and CD8 cells participate in host defence to cryptococcus (56,69) and histoplasma (70).…”

Section: Recruited Mechanisms Of Host Defencementioning

confidence: 99%

Host Defence to Pulmonary Mycosis

Mody

Warren

1999

Canadian Journal of Infectious Diseases and Medical Microbiolog

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By separating fungi based on the host immune defects that predispose to disease, in conjunction with traditional divisions based on the geographic distribution of fungi, clinicians are able to focus their diagnostic efforts and to identify fungal pathogens better. In addition, an understanding of the normal host defense mechanisms that serve to control fungal infections is essential to the development of novel antifungal therapies.

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“…Cell-mediated immune responses are of primary importance in protective immunity to histoplasmosis and in diseases caused by other facultative intracellular parasites. In histoplasmosis the evidence for this concept is provided by several studies: first, lymphocytes from mice immunized by sublethal infection with Histoplasma capsulatum can mediate suppression of growth of the fungus within normal macrophages (7,8); second, the higher susceptibility of congenitally athymic mice (24) or normal mice following treatment with antilymphocyte serum and cytotoxic agents (1,6); and third, the adopive transfer of anti-Histoplasma immunity by spleen and peritoneal cells from immunized donors (9, 19,21,25).…”

Section: Introductionmentioning

confidence: 99%

“…capsulatum (19). The immunity is transferable to syngeneic recipients by immune lymphoid cells but not with immune serum (19,21). The immune spleen and peritoneal cells which confer immunity to histoplasmosis are Tlymphocytes and their active proliferation in recipients is necessary for the expression of anti-Histoplasma immunity.…”

Section: Introductionmentioning

confidence: 99%

Cellular Mediators of Anti‐Histoplasma Immunity: I. Protective Immunity and Cellular Changes in Spleens of Mice Immunized by Sublethal Infection with Yeast Cells of Histoplasma capsulatum*/ Zelluläre Mediatoren der Anti‐Histoplasma‐Immunität: I. Protektive Immunität und Zellveränderungen in der Milz von Mäusen, immunisiert durch subletale Infektion mit Histoplasma capsulatum in der Hefephase

Khardori¹,

Behren²,

Chaudhary³

et al. 1986

Mycoses

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Summary: The relationship between protective immunity and cellular changes in spleens of mice immunized by subcutaneous inoculation with live yeast cells of Histoplasma capsulatum was investigated. 3H‐thymidine uptake by spleens of immune mice increased gradually reaching a peak of 207.6 × 103 ± 8.3 CPM/spleen on day 14, showed a decline on day 21 (p < 0.001) and was not significantly different from controls at 42 and 63 days. Changes in spleen weight and total number of spleen cells of immune mice showed a similar pattern. In vitro DNA synthesis by immune spleen cells increased to a maximum of 41.1 × 103 ± 0.5 CPM/107 splenocytes on day 14 and thereafter showed a gradual decline. Although the organisms disseminated from the inoculation site, there was only limited multiplication and all organs were free of the pathogen by day 63. Histopathologically, splenic hyperplasia, most significant at 14 days, was also seen in infected mice. Adoptive transfer of 108 spleen cells from donors up to 7 days post‐immunization did not protect the recipients whereas 90–100% protection was observed when 14–105 days immunized donors were used. These results indicate that the development of immunity is accompanied by cellular proliferation in the spleen, leading to the appearance of immunologically committed cellular mediators capable of conferring protective immu Zusammenfassung: Es wurden die Beziehungen zwischen protektiver Immunität und Zellveränderungen in der Milz von Mäusen untersucht, die subkutan mit lebenden Histoplasma capsulatum‐Zellen in der Hefephase inokuliert worden waren. Die 3H‐Thymidin‐Aufnahme in der Milz immuner Mäuse stieg an und erreichte am 14. Tag ein Maximum mit 207.6 × 103 ± 8.3 CPM/Milz, zeigte am 21. Tag einen Abfall (p < 0.001) und unterschied sich signifikant von den Kontrollen nach 42 und 63 Tagen. Die Veränderungen im Milzgewicht und in der Gesamtzahl der Milzzellen immuner Mäuse zeigten ein ähnliches Verlaufsmuster. Die in vitro‐DNA‐Synthese immuner Milzzellen erreichte am 14. Tag ein Maximum von 41.1 × 103 ± 0.5 CPM/107 Splenozyten und fiel danach wieder ab. Obgleich sich die Pilze vom Inokulationsort ausbreiteten, war nur eine begrenzte Vermehrung zu beobachten, und am 63. Tag waren alle Organe erregerfrei. Histopathologisch wurde in den infizierten Mäusen eine Milzhyperplasie beobachtet, die am 14. Tag ihren Höhepunkt erreichte. Die Übertragung von 108 Milzzellen von Spendern vermochte die Empfánger bis zum 7. Tag nach der Immunisierung nicht zu schÜtzen, während mit immunisierten Spendern ein 90–100%iger Schutz 14–105 Tage nach der Immunisierung erzielt wurde. Diese Ergebnisse belegen, daß der Aufbau der Immunität von einer Zellvermehrung in der Milz begleitet wird, die zum Auftreten immunologisch kompetenter Zellmediatoren fÜhrt mit der Fähigkeit, protektive Immunität zu Übertragen.

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Adoptive transfer of immunity from mice immunized with ribosomes or live yeast cells of Histoplasma capsulatum

Cited by 47 publications

References 17 publications

Immunoregulatory Responses in Experimental Disseminated Histoplasmosis: Lymphoid Organ Histopathology and Serological Studies

Immunoregulatory Responses in Experimental Disseminated Histoplasmosis: Lymphoid Organ Histopathology and Serological Studies

Host Defence to Pulmonary Mycosis

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