Adipose tissue-derived stem cells in clinical applications

Pikuła, Michał; Marek-Trzonkowska, Natalia; Wardowska, Anna; Renkielska, Alicja; Trzonkowski, Piotr

doi:10.1517/14712598.2013.823153

Cited by 76 publications

(61 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The hASCs were selected for this study due to their multipotency and ability to differentiate into various lineages 42,43 . Adipose tissue is an abundant source of autologous adult stem cells, which may be implicated in variety of cell-based therapies for the regeneration of acute and chronically damaged tissues 44,45 . To ensure that developed injectable hydrogels, reinforced with a-CNCs have preferential cell supportive properties we tested the ability of hASCs to adhere on the surface of produced nanocomposites and HAX.…”

Section: Cellular Interactions With Ha-cncs Hydrogelsmentioning

confidence: 99%

Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications

et al. 2015

View full text Add to dashboard Cite

Injectable hyaluronic acid (HA)-based hydrogels compose a promising class of materials for tissue engineering and regenerative medicine applications. However, their limited mechanical properties restrict the potential range of application. In this study, cellulose nanocrystals (CNCs) were employed as nanofillers in a fully biobased strategy for the production of reinforced HA nanocomposite hydrogels. Herein we report the development of a new class of injectable hydrogels composed of adipic acid dihydrazide-modified HA (ADH-HA) and aldehyde-modified HA (a-HA) reinforced with varying contents of aldehyde-modified CNCs (a-CNCs). The obtained hydrogels were characterized in terms of internal morphology, mechanical properties, swelling, and degradation behavior in the presence of hyaluronidase. Our findings suggest that the incorporation of a-CNCs in the hydrogel resulted in a more organized and compact network structure and led to stiffer hydrogels (maximum storage modulus, E', of 152.4 kPa for 0.25 wt % a-CNCs content) with improvements of E' up to 135% in comparison to unfilled hydrogels. In general, increased amounts of a-CNCs led to lower equilibrium swelling ratios and higher resistance to degradation. The biological performance of the developed nanocomposites was assessed toward human adipose derived stem cells (hASCs). HA-CNCs nanocomposite hydrogels exhibited preferential cell supportive properties in in vitro culture conditions due to higher structural integrity and potential interaction of microenvironmental cues with CNC's sulfate groups. hASCs encapsulated in HA-CNCs hydrogels demonstrated the ability to spread within the volume of gels and exhibited pronounced proliferative activity. Together, these results demonstrate that the proposed strategy is a valuable toolbox for fine-tuning the structural, biomechanical, and biochemical properties of injectable HA hydrogels, expanding their potential range of application in the biomedical field.

show abstract

Section: Cellular Interactions With Ha-cncs Hydrogelsmentioning

confidence: 99%

Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications

et al. 2015

View full text Add to dashboard Cite

show abstract

“…SVF isolates typically contain many components also found in adipose tissue including adipocytes, fibroblasts, preadipocytes, tissue resident macrophages, and vascular constituents (Baer and Geiger 2012; Aronowitz and Ellenhorn 2013; Gimble et al 2011). Most importantly, both adipose tissue and SVF provide a source of mesenchymal stem cells (MSCs) that do not elicit an immunological response, particularly if autologous ASCs are used during point-of-care applications (McIntosh et al 2009; Pikuła et al 2013). Of the three main sources of MSCs currently used for human studies and therapies—bone marrow, umbilical cord blood, and adipose tissue—ASCs show important differences from bone-marrow derived MSCs in regard to harvest and cell yield, despite similarities in morphology and phenotype.…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles

et al. 2016

View full text Add to dashboard Cite

BackgroundAdipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen.ResultsFlow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification.ConclusionsThe main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3712-1) contains supplementary material, which is available to authorized users.

show abstract

“…Adipose-derived stem cells (ASCs) proved to have significant biological properties and considerable therapeutic potential [71]. It seems that ASCs, in their physiological state, remain rather quiescent, but under stimulation (such as a wound, a transplant, or in vitro culture) exert pro-regenerative capabilities.…”

Section: Cell Therapies For Chronic Wound Treatmentmentioning

confidence: 99%

“…ASCs are a promising tool for treatment of chronic (e.g. ulcers) and oncological wounds [71]. Riggotti et al [77] in a pilot clinical study in humans showed that injection of autologous lipopaspirates, which contain ASCs, can be effective in treating late side effects of radiotherapy.…”

Section: Cell Therapies For Chronic Wound Treatmentmentioning

confidence: 99%

Wound healing complications in oncological patients: perspectives for cellular therapy

Deptuła

Zieliński

Wardowska

et al. 2019

pdia

Self Cite

View full text Add to dashboard Cite

A b s t r a c tVarious types of cancer are nowadays a serious medical and social problem and a great challenge for modern medicine. The majority of anticancer therapy is based on traditional chemotherapy and radiotherapy. Both of these highly non-specific types of treatment have a number of serious side effects including wound healing complications. Radiotherapy and chemotherapy mostly affect rapidly dividing skin cells (e.g. keratinocytes), as well as fibroblasts, melanocytes, endothelial and immune cells. Currently, there are many strategies to improve wound healing in oncological patients, including various types of dressings, biomaterials, growth factors, and cell therapies.

show abstract

Adipose tissue-derived stem cells in clinical applications

Cited by 76 publications

References 91 publications

Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications

Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications

Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles

Wound healing complications in oncological patients: perspectives for cellular therapy

Contact Info

Product

Resources

About