Adipocyte morphology and leptin signaling in rat offspring from mothers supplemented with flaxseed during lactation

Figueiredo, Mariana Sarto; Passos, Magna Cottini da Fonseca; Trevenzoli, Isis Hara; Troina, Aline Andrade; Carlos, Aluana Santana; Nascimento-Saba, Celly Cristina Alves do; Manhães, Alex C.; Oliveira, Elaine de; Lisboa, Patrícia Cristina; Moura, Egberto Gaspar de

doi:10.1016/j.nut.2011.07.002

Cited by 13 publications

(3 citation statements)

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“…For women who eat about 800 g of food per d, this would represent 200 g of flaxseed and would be equivalent to about nine spoonfuls of flaxseed per d. This amount is easily reached if women ate three spoonfuls of flaxseed with each main meal. Daily consumption of 200 g of flaxseed would represent 20 % of the recommended daily fibre intake (25 g/d) for women, and this amount was used in our previous experimental studies showing negative health effects in pups when they become adults ( 8 – 10 ) . At adulthood, male offspring whose mothers received dietary flaxseed (25 %) exhibited higher body weight despite lower body visceral fat.…”

Section: Discussionmentioning

confidence: 99%

“…For Western blot assays, samples of the liver, hypothalamus and pituitary gland were taken from rats and immediately frozen in liquid N 2 ( 10 ) . Briefly, to obtain cell extracts, tissues were homogenised in ice-cold lysis buffer (50 m m -HEPES, 1 m m -MgCl 2 , 10 m m -EDTA, Triton X-100 1 %, pH 6·4) containing the following protease inhibitors: 10 mg/ml of aprotinin; 10 mg/ml of leupeptin; 2 mg/ml of pepstatin; 1 m m -phenylmethylsulfonyl fluoride (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously demonstrated that maternal intake of flaxseed (25 %) during lactation leads to lower body fat mass, hyperleptinaemia, hypoinsulinaemia, increased insulin sensitivity, increased pituitary expression of leptin receptor, phosphorylated signal transducer and activator of transcription 3, and lower serum T3 concentrations in weaned pups. In adulthood, these animals were programmed for an increased adipocyte area in both visceral and subcutaneous adipose tissues, hyperglycaemia, hyperinsulinaemia, hypoadiponectinaemia and increased insulin resistance, lower serum T4 (thyroxine), type 1 deiodinase (D1) and type 2 deiodinase (D2) activities in the thyroid, higher D2 activity in brown adipose tissue, and increased expression of leptin receptor in the thyroid ( 8 – 10 ) . Probably the majority of these effects were mediated by bioactive compounds present in flaxseed, such as α-linolenic acid and SDG.…”

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Maternal flaxseed diet during lactation changes adrenal function in adult male rat offspring

et al. 2015

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Flaxseed (Linum usitatissimum L.) has been a focus of interest in the field of functional foods because of its potential health benefits. However, we hypothesised that maternal flaxseed intake during lactation could induce several metabolic dysfunctions in adult offspring. In the present study, we aimed to characterise the adrenal function of adult offspring whose dams were supplemented with whole flaxseed during lactation. At birth, lactating Wistar rats were divided into two groups: rats from dams fed the flaxseed diet (FLAX) with 25 % of flaxseed and controls dams. Pups received standard diet after weaning and male offspring were killed at age 180 days old to collect blood and tissues. We evaluated body weight and food intake during development, corticosteronaemia, adrenal catecholamine content, hepatic cholesterol, TAG and glycogen contents, and the protein expression of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), 11-b-hydroxysteroid dehydrogenase type 1 (11b-HSD1) and adrenaline b2 receptor at postnatal day 180 (PN180). After weaning, pups from the FLAX group had a higher body weight (þ 10 %) and food intake (þ 10 %). At PN180, the FLAX offspring exhibited higher serum corticosterone (þ 48 %) and lower adrenal catecholamine (2 23 %) contents, lower glycogen (2 30 %), higher cholesterol (4-fold increase) and TAG (3-fold-increase) contents in the liver, and higher 11b-HSD1 (þ62 %) protein expression. Although the protein expression of hypothalamic CRH was unaffected, the FLAX offspring had lower protein expression of pituitary ACTH (2 34 %). Therefore, induction of hypercorticosteronaemia by dietary flaxseed during lactation may be due to an increased hepatic activation of 11b-HSD1 and suppression of ACTH. The changes in the liver fat content of the FLAX group are suggestive of steatosis, in which hypercorticosteronaemia may play an important role. Thus, it is recommended that lactating women restrict the intake of flaxseed during lactation.

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confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Maternal flaxseed diet during lactation changes adrenal function in adult male rat offspring

et al. 2015

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Maternal cinnamon intake during lactation led to visceral obesity and hepatic metabolic dysfunction in the adult male offspring

et al. 2018

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Flaxseed bioactive compounds change milk, hormonal and biochemical parameters of dams and offspring during lactation

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Figueiredo

Passos

et al. 2012

Food and Chemical Toxicology

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We evaluated maternal intake of SDG (secoisolariciresinol diglucoside), a compound from flaxseed, and flaxseed oil+SDG on biochemical and hormonal parameters of dams and male and female offspring during lactation. Dams were fed a standard diet (C); diet added 40 mg of SDG/100g diet (SDG) or diet added 40 mg of SDG/100g diet and 7% of flaxseed oil (OLSDG). SDG and OLSDG dams showed hyperprolactinemia. The OLSDG milk had lower lactose and protein, while the SDG milk had lower protein on the 14th day of lactation. At 14 days, OLSDG male and female pups showed lower body mass, SDG and OLSDG male pups had hypoprolactinemia and lower body fat mass, but higher visceral fat mass (VFM) and hypertriglyceridemia. At 21 days, male SDG and OLSDG presented hypotriglyceridemia. At 14 days, SDG and OLSDG female offspring showed higher serum 17-β estradiol (E2); OLSDG presented hypercholesterolemia and SDG presented hypertriglyceridemia. At 21 days, SDG and OLSDG female pups showed hypotriglyceridemia and OLSDG shower lower E2. Both maternal treatments changes maternal metabolism as well as hormonal and biochemical parameters of the offspring, which are gender-dependent. Maternal hyperprolactinemia may act as an imprint factor responsible for the hormonal and metabolic changes observed in the pups.

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Adipocyte morphology and leptin signaling in rat offspring from mothers supplemented with flaxseed during lactation

Cited by 13 publications

References 59 publications

Maternal flaxseed diet during lactation changes adrenal function in adult male rat offspring

Maternal flaxseed diet during lactation changes adrenal function in adult male rat offspring

Maternal cinnamon intake during lactation led to visceral obesity and hepatic metabolic dysfunction in the adult male offspring

Flaxseed bioactive compounds change milk, hormonal and biochemical parameters of dams and offspring during lactation

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