Adhesive Properties and Other Virulence Factors in Yersinia Ruckeri

Romalde, Jesús L.; Lemos, Manuel L.; Conchas, Ramón F.; Bandı́n, Isabel; Toranzo, Alicia E.

doi:10.1016/b978-0-12-550755-4.50018-5

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…Lack of correlation between SAT and BATH test results was also observed by Santos et al (1990) and by Lee & Yii (1996). Lack of correlation between pathogenicity and hydrophobicity has also been reported by Romalde et al (1990), who concluded that cell surface properties were not good criteria for estimating pathogenicity of the fish pathogen Yersinia ruckerii. Difficul-237 Table 3.…”

Section: Discussionmentioning

confidence: 79%

Virulence of luminous vibrios to Artemia franciscana nauplii

Soto-Rodríguez¹,

Roque²,

Lizárraga-Partida³

et al. 2003

Dis. Aquat. Org.

107

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From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 10 5 to 10 6 CFU ml -1 compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) by the Artemia nauplii varied from less than 10 to 10 3 CFU nauplius -1 and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significant correlations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest that virulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.

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Section: Discussionmentioning

confidence: 79%

Virulence of luminous vibrios to Artemia franciscana nauplii

Soto-Rodríguez¹,

Roque²,

Lizárraga-Partida³

et al. 2003

Dis. Aquat. Org.

107

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“…These differences have been attributed to factors such as adhesive properties (Romalde et al, 1990), protease and haemolytic activities (Romalde & Toranzo, 1993), iron acquisition systems (Davies, 1991 ;Romalde & Toranzo, 1993) and the presence of a heat-sensitive factor (Doug et al, 1992 ;Everlyn, 1996). However, until now, neither of these factors has been confirmed to be clearly involved in virulence.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Romalde et al (1990) have shown that some properties related to virulence, such as haemagglutination, hydrophobicity and different cell-surface components, are not relevant to the pathogenesis of Y. ruckeri. The early development of a vaccine in the prevention of this disease is probably one of the reasons why virulence determinants have not been studied in depth.…”

Section: Ferna! Ndez and Othersmentioning

confidence: 99%

Isolation and analysis of a protease gene with an ABC transport system in the fish pathogen Yersinia ruckeri: insertional mutagenesis and involvement in virulence a aThe GenBank accession numbers for the sequences reported in this paper are AJ318052 (yrp1) and AJ421517 (yrpDEF and inh).

et al. 2002

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Yersinia ruckeri is a Gram-negative pathogen that causes enteric redmouth disease in salmonids. A gene from Y. ruckeri encoding an extracellular protease termed yrp1 (Yersinia ruckeri protease 1) was cloned from a Sau3AI library constructed in pUC19 and analysed in gelatin-supplemented medium. The nucleotide sequence of the yrp1 gene indicated an ORF encoding a protein of 477 aa. On the basis of the high degree of homology in the amino acid sequence as well as its conservative motifs, this protein was included within the serralysin metalloendopeptidase subfamily (EC 3.4.24.12). The yrp1 Nterminal sequence showed a 14 aa propeptide followed by a 10 aa sequence identical to the one deduced previously from the 47 kDa purified protease. Additional results demonstrated that the yrp1 gene encodes the 47 kDa protein. In contrast to other Yersinia species, the yrp1 protease is secreted by a type I Gram-negative bacterial ABC exporter protein secretion system composed of three genes termed yrpD, yrpE and yrpF, and a protease inhibitor inh. The development of genetic methods for this species has allowed the exploration of the organization and the putative role of the Yrp1 genetic locus. Thus, site-directed insertion mutations into the yrp1 and the yrpE genes were constructed by the integration of the mobilizable suicide vector pIVET8 containing internal portions of both coding sequences. Complementation studies of those mutants with different loci indicated that they are organized as a single operon. The mutant strains lacked protease activity as well as the Yrp1 protein and, although physiologically similar to the parental strain when growing on nutrient broth medium, they were attenuated in virulence when bacteria were injected intraperitoneally into rainbow trout (Oncorhynchus mykiss). This is the first report of defined mutations in Y. ruckeri to show the implication of a factor such as an extracellular protease in pathogenesis.

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“…It causes enteric redmouth disease (ERM), one of the most threatening diseases occurring in pisciculture and responsible for important economic losses [1,2]. Its classification in the genus Yersinia is controversial [3][4][5], and the species is sometimes defined as a "bacterial species looking for a final genus home".…”

Section: Introductionmentioning

confidence: 99%

Use of pulsed field gel electrophoresis to size the chromosome of the bacterial fish pathogen Yersinia ruckeri

Romalde

1991

FEMS Microbiology Letters

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Field inversion gel electrophoresis (FIGE) and contour-clamped homogeneous field (CHEF) electrophoresis were used to analyse the chromosome of Yersinia ruckeri. The 8 base-pair recognition endonucleases, NotI and SfiI, generated less than 47 DNA fragments whose size and distribution were appropriate for pulsed field separation. Each isolate displayed a characteristic restriction pattern, with about 20% of bands in common. Depending on the strain used, the estimated genome size for this bacterial fish pathogen ranged from 4460 to 4770 kilobase pairs.

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Adhesive Properties and Other Virulence Factors in Yersinia Ruckeri

Cited by 10 publications

References 26 publications

Virulence of luminous vibrios to Artemia franciscana nauplii

Virulence of luminous vibrios to Artemia franciscana nauplii

Isolation and analysis of a protease gene with an ABC transport system in the fish pathogen Yersinia ruckeri: insertional mutagenesis and involvement in virulence a aThe GenBank accession numbers for the sequences reported in this paper are AJ318052 (yrp1) and AJ421517 (yrpDEF and inh).

Use of pulsed field gel electrophoresis to size the chromosome of the bacterial fish pathogen Yersinia ruckeri

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