Adherence compounds in embryo transfer media for assisted reproductive technologies

Abstract

There is evidence of an improved clinical pregnancy rate with the use of adherence compounds in ART cycles but no evidence of an effect on live birth rate. The increase in multiple pregnancy rate may be the result of the use of a combination of an adherence compound and a policy of transferring more than one embryo. Further studies of adherence compounds with single embryo transfer need to be undertaken.

Clinical efficacy of hyaluronate-containing embryo transfer medium in IVF/ICSI treatment cycles: a cohort study

“…HA-rich medium showed a significant increase in the odds of achieving a clinical pregnancy and live birth relative to using low HA medium (G 2+ ) for ET, with similar effects seen for both durations (long and short HA exposures). This observation is consistent with most previous studies evaluating the efficacies of so-called ‘adherence compounds’ in ART ( Valojerdi et al , 2006 ; Friedleri et al , 2007 ; Urman et al , 2008 ; Nakagawa et al , 2012 ; Bontekoe et al , 2014 ). Conversely, other studies ( Simon et al , 2003 ; Hambiliki et al , 2010 ; Fancsovits et al , 2015 ) failed to identify any statistically significant improvement in clinical pregnancy, implantation and LBE rates following the use of HA-rich medium.…”

“…We were unable to show an impact of HA-rich medium, for either duration of exposure, on any of these outcome measures. The small trends, towards increased risk of multiple births and biochemical pregnancies with the use of HA-rich medium, although not reaching statistical significance in the present study, are consistent with other studies ( Chen et al , 2001 ; Friedleri et al , 2007 ; Bontekoe et al , 2014 ; Nishihara and Morimoto, 2017 ). Finally, we also analysed BW and GA as part of our safety evaluation of the impact of ART and, reassuringly, saw no differences in any measure with HA-rich medium for either exposure time.…”

Clinical efficacy of hyaluronate-containing embryo transfer medium in IVF/ICSI treatment cycles: a cohort study

“…HA-rich medium showed a significant increase in the odds of achieving a clinical pregnancy and live birth relative to using low HA medium (G 2+ ) for ET, with similar effects seen for both durations (long and short HA exposures). This observation is consistent with most previous studies evaluating the efficacies of so-called ‘adherence compounds’ in ART ( Valojerdi et al , 2006 ; Friedleri et al , 2007 ; Urman et al , 2008 ; Nakagawa et al , 2012 ; Bontekoe et al , 2014 ). Conversely, other studies ( Simon et al , 2003 ; Hambiliki et al , 2010 ; Fancsovits et al , 2015 ) failed to identify any statistically significant improvement in clinical pregnancy, implantation and LBE rates following the use of HA-rich medium.…”

“…We were unable to show an impact of HA-rich medium, for either duration of exposure, on any of these outcome measures. The small trends, towards increased risk of multiple births and biochemical pregnancies with the use of HA-rich medium, although not reaching statistical significance in the present study, are consistent with other studies ( Chen et al , 2001 ; Friedleri et al , 2007 ; Bontekoe et al , 2014 ; Nishihara and Morimoto, 2017 ). Finally, we also analysed BW and GA as part of our safety evaluation of the impact of ART and, reassuringly, saw no differences in any measure with HA-rich medium for either exposure time.…”

Evaluation of transfer media containing different concentrations of hyaluronan for human in vitro fertilization

“…However, the addition of HA might improve the developmental capacity of embryos under in vitro conditions. Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not.…”

“…Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not. The increasing number of multiple births might be the result of the use of a combination of an adherence compound and a policy of transferring more than one embryo.…”

Blastocyst utilization rates after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes

“…Hyaluronan in combination with human serum albumin (HSA) has been found to increase embryo development and to improve embryo cryosurvival [35]. In addition, evidence suggests improved clinical pregnancy and live birth rates with the use of hyaluronic acid as an adherence compound in the embryo transfer medium [36]. A putative beneficial effect of hyaluronan in Sage one-step on blastocyst development and pregnancy rates could not be demonstrated in the present study.…”

Clinical efficacy of hyaluronate-containing embryo transfer medium in IVF/ICSI treatment cycles: a cohort study

“…HA-rich medium showed a significant increase in the odds of achieving a clinical pregnancy and live birth relative to using low HA medium (G 2+ ) for ET, with similar effects seen for both durations (long and short HA exposures). This observation is consistent with most previous studies evaluating the efficacies of so-called ‘adherence compounds’ in ART ( Valojerdi et al , 2006 ; Friedleri et al , 2007 ; Urman et al , 2008 ; Nakagawa et al , 2012 ; Bontekoe et al , 2014 ). Conversely, other studies ( Simon et al , 2003 ; Hambiliki et al , 2010 ; Fancsovits et al , 2015 ) failed to identify any statistically significant improvement in clinical pregnancy, implantation and LBE rates following the use of HA-rich medium.…”

“…We were unable to show an impact of HA-rich medium, for either duration of exposure, on any of these outcome measures. The small trends, towards increased risk of multiple births and biochemical pregnancies with the use of HA-rich medium, although not reaching statistical significance in the present study, are consistent with other studies ( Chen et al , 2001 ; Friedleri et al , 2007 ; Bontekoe et al , 2014 ; Nishihara and Morimoto, 2017 ). Finally, we also analysed BW and GA as part of our safety evaluation of the impact of ART and, reassuringly, saw no differences in any measure with HA-rich medium for either exposure time.…”

Evaluation of transfer media containing different concentrations of hyaluronan for human in vitro fertilization

“…However, the addition of HA might improve the developmental capacity of embryos under in vitro conditions. Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not.…”

“…Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not. The increasing number of multiple births might be the result of the use of a combination of an adherence compound and a policy of transferring more than one embryo.…”

Blastocyst utilization rates after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes

“…Hyaluronan in combination with human serum albumin (HSA) has been found to increase embryo development and to improve embryo cryosurvival [35]. In addition, evidence suggests improved clinical pregnancy and live birth rates with the use of hyaluronic acid as an adherence compound in the embryo transfer medium [36]. A putative beneficial effect of hyaluronan in Sage one-step on blastocyst development and pregnancy rates could not be demonstrated in the present study.…”

Clinical efficacy of hyaluronate-containing embryo transfer medium in IVF/ICSI treatment cycles: a cohort study

“…HA-rich medium showed a significant increase in the odds of achieving a clinical pregnancy and live birth relative to using low HA medium (G 2+ ) for ET, with similar effects seen for both durations (long and short HA exposures). This observation is consistent with most previous studies evaluating the efficacies of so-called ‘adherence compounds’ in ART ( Valojerdi et al , 2006 ; Friedleri et al , 2007 ; Urman et al , 2008 ; Nakagawa et al , 2012 ; Bontekoe et al , 2014 ). Conversely, other studies ( Simon et al , 2003 ; Hambiliki et al , 2010 ; Fancsovits et al , 2015 ) failed to identify any statistically significant improvement in clinical pregnancy, implantation and LBE rates following the use of HA-rich medium.…”

“…We were unable to show an impact of HA-rich medium, for either duration of exposure, on any of these outcome measures. The small trends, towards increased risk of multiple births and biochemical pregnancies with the use of HA-rich medium, although not reaching statistical significance in the present study, are consistent with other studies ( Chen et al , 2001 ; Friedleri et al , 2007 ; Bontekoe et al , 2014 ; Nishihara and Morimoto, 2017 ). Finally, we also analysed BW and GA as part of our safety evaluation of the impact of ART and, reassuringly, saw no differences in any measure with HA-rich medium for either exposure time.…”

Evaluation of transfer media containing different concentrations of hyaluronan for human in vitro fertilization

“…However, the addition of HA might improve the developmental capacity of embryos under in vitro conditions. Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not.…”

“…Bontekoe et al showed that clinical pregnancy and live birth rates were improved with the use of functional concentrations of HA in ART cycles . However, the evidence obtained was of moderate quality, and also this study did not show whether low concentrations of HA are sufficient or not. The increasing number of multiple births might be the result of the use of a combination of an adherence compound and a policy of transferring more than one embryo.…”

Blastocyst utilization rates after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes

“…Hyaluronan in combination with human serum albumin (HSA) has been found to increase embryo development and to improve embryo cryosurvival [35]. In addition, evidence suggests improved clinical pregnancy and live birth rates with the use of hyaluronic acid as an adherence compound in the embryo transfer medium [36]. A putative beneficial effect of hyaluronan in Sage one-step on blastocyst development and pregnancy rates could not be demonstrated in the present study.…”