Adherence and intracellular parasitism of   in Vero cells

Hanna, Samira Abdallah; Silva, Juliana L Monteiro da; Giannini, Maria José Soares Mendes

doi:10.1016/s1286-4579(00)00390-7

Cited by 79 publications

(90 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They also provide a barrier to micro-organisms, toxins and various antigens. Infection of host mucous cells may occur through the spread of P. brasiliensis yeast cells in this type of tissue, unlike in the primary infection in which conidia/mycelial fragments are the infectious particles.The ability of P. brasiliensis to adhere to and invade nonprofessional phagocytes or epithelial cells has been recognized in previous studies (Hanna et al, 2000;Lenzi et al, 2000; Mendes-Giannini et al, 1994, 2004. To establish infection in these cells, it is necessary for P. brasiliensis to be able to modify its expressed genes to adapt to changes in the host environment, so the identification of differentially expressed genes during the parasite-host interaction may help to elucidate the mechanisms used for the survival, resistance and growth of this fungus.…”

mentioning

confidence: 84%

See 1 more Smart Citation

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

View full text Add to dashboard Cite

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, one of the most important systemic fungal diseases in Latin America. This initiates in lung tissue and can subsequently disseminate to other tissues. Clinical manifestations range from localized forms to disseminated disease that can progress to lethality, probably depending on the relationships among the virulence of the fungus, the immune response and the ability to interact with the surface structures and invade epithelial cells and mononuclear cells of the host. It is generally regarded as a multifocal disease, with oral lesions as the prominent feature. The aim of this study was to evaluate P. brasiliensis yeast infection in normal oral keratinocytes (NOKs). The differential expression of mRNAs and proteins was also determined when the fungus was placed in contact with the cell in order to characterize differentially expressed genes and proteins during P. brasiliensis infection. After contact with NOKs, the fungus appeared to induce alterations in the cells, which showed cellular extensions and cavitations, probably resulting from changes in the actin cytoskeleton seen at 5 and 8 h after infection. Levels of protein expression were higher after reisolation of the fungus from infected NOK culture compared with culture of the fungus in medium. The analysis identified transcripts related to 19 proteins involved in different biological processes. Transcripts were found with multiple functions including induction of cytokines, protein metabolism, alternative carbon metabolism, zinc transport and the stress response during contact with NOKs. The proteins found suggested that the yeast was in a stress situation, as indicated by the presence of RDS1. Nevertheless, the yeast seemed to be proliferating and metabolically active, as shown by the presence of a proteasome, short-chain acetylator, glucosamine-6-phosphate isomerase and ADP/ATP carrier transcripts. Additionally, metabolic pathways may have been activated in order to eliminate toxic substances from the cell as a zinc transporter was detected, which is a potential target for the development of future drugs. INTRODUCTIONParacoccidioides brasiliensis is a thermodimorphic pathogenic fungus, the aetiological agent of paracoccidioidomycosis (PCM), an endemic disease geographically limited to Latin America and one of the most prevalent human deep systemic mycoses found in Brazil, Colombia and Venezuela (San-Blas et al., 2002). The transition from mycelium at 25 u C to yeast at 37 u C is essential for P. brasiliensis to establish the disease (Franco et al., 1997). P. brasiliensis may be inhaled and, once in the lungs, the fungus initiates a process of morphological transition into the pathogenic yeast form (San-Blas et al., 2002).PCM has a multiplicity of clinical presentations, from cutaneous to systemic forms, and can attack various tissues, especially the lungs (Benard & Franco, 2007). Oral mucosal lesions are commonly multiple, with a granular, mulberry-like surface and microulcerations, and ...

show abstract

mentioning

confidence: 84%

“…brasiliensis to adhere to and invade epithelial cells (Mendes-Giannini et al, 2008), and the adhesion phenomenon is variable depending on the isolate (Andreotti et al, 2005;Hanna et al, 2000). Adhesion of P. brasiliensis to host tissues is a crucial step in the establishment of PCM.…”

Section: R Peres Da Silva and Othersmentioning

confidence: 99%

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…The ability to adhere is a biological phenomenon that is widely distributed and shared by many pathogens, enabling the colonization of their respective habitats. 15,16 Some adhesins have been described for P. brasiliensis, such as gp43, 17,18 glyceraldehyde-3-phosphate dehydrogenase, 19 triose phosphate isomerase, 20 enolase, 21,22 a 32-Kda hydrolase, 23,24 malate synthase, 25 isocitrate lyase 26 and the 14-3-3 protein (30 KDa) 27,28 ; this last adhesin is the focus of the present study.…”

Section: Introductionmentioning

confidence: 95%

Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

et al. 2016

View full text Add to dashboard Cite

“…Similarly, different authors have reported a positive correlation between adhesion of fungal pathogens and virulence. For example, pathogenic strains of Aspergillus fumigatus bound significantly more fibronectin than non-pathogenic strains (Wasylnka & Moore, 2000) and, among different strains of P. brasiliensis, the most virulent ones showed a stronger adherence to epithelial cells (Mendes-Giannini et al, 2006;Hanna et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Study of independent isolates of Paracoccidioides brasiliensis revealed that the strains that were more virulent in animals exhibited enhanced adherence to extracellular matrix proteins in vitro (Hanna et al, 2000;Mendes-Giannini et al, 2006). However, to our knowledge such studies have never been conducted for S. schenckii.…”

Section: Introductionmentioning

confidence: 96%

Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii

et al. 2009

View full text Add to dashboard Cite

The virulence of four Sporothrix schenckii isolates was compared in a murine model of sporotrichosis, together with the protein pattern of the yeast cell surface and the capacity to bind the extracellular matrix protein fibronectin. Virulence was determined by the mortality rate, fungal burden and histopathology. Two clinical isolates were more virulent for C57BL/6 mice, but no direct correlation was seen between virulence and the clinical or environmental origin of the isolates. The lowest virulence was observed for an isolate recovered from a patient with meningeal sporotrichosis. Although all isolates could effectively disseminate, the dissemination patterns were not similar. Using flow cytometry analysis, we investigated the interaction of all the strains with fibronectin, and showed that the binding capacity correlated with virulence. Western blot analysis of S. schenckii cell wall extracts revealed positive bands for fibronectin in the range of 37-92 kDa. The 70 kDa adhesin was also recognized by a protective monoclonal antibody raised against a gp70 antigen of S. schenckii (mAb P6E7). Confocal microscopy confirmed the colocalization of fibronectin and mAb P6E7 on the yeast cell surface. To our knowledge, this is the first report identifying adhesins for fibronectin on the surface of this human pathogen.

show abstract

Adherence and intracellular parasitism of in Vero cells

Cited by 79 publications

References 28 publications

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii

Contact Info

Product

Resources

About