Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Abstract

How to cite:Please refer to published version for the most recent bibliographic citation information. If a published version is known of, the repository item page linked to above, will contain details on accessing it.

Amino acid motifs for the identification of novel protein interactants

“…The application of carefully curated amino acid motifs to model and non-model organisms across the tree of life, therefore offers an exciting and currently undervalued opportunity for the discovery of hidden or unexpected ligand interacting sites in new and/or well-characterized proteins [107] , [164] . This is in addition to the many examples of enzymatic moonlighting sites already identified [25] , [27] , [32] , [36] .…”

“…Discovering protein interactants beyond those involved in catalysis, could reveal novel signaling mechanisms or revisit existing ones [38] , [107] . One such example is the recent identification of an AC operating in the TIR1/AFB auxin receptors which participate in the classical nuclear auxin signal transduction pathway that affects auxin-dependent root growth inhibition and gravitropism [164] , [165] . A better understanding of interactomes is also likely to uncover complex hidden mechanisms that operate at a systems level [166] , [167] .…”

Three Novel Adenylate Cyclase Genes Show Significant Biological Functions in Plant

“…Sutherland et al first identified cAMP in 1956 and demonstrated it to be a second messenger in the liver, , Kandel further discovered that cAMP could switch short-term memory into long-term memory and relieve the fatigue of brain cells, , and they were awarded the Nobel Prize in Physiology or Medicine in 1971 and 2000, respectively. Subsequent studies showed that cAMP participates in a wide range of cell metabolism regulations and functions as an intracellular second messenger in a variety of organisms. − In plants, cAMP has been demonstrated to function as a second messenger involved in pollen tube growth and reorientation in maize and root growth in Arabidopsis thaliana. , …”

ABLs and TMKs are co-receptors for extracellular auxin

“…Auxin regulates nearly every aspect of plant growth, development, and responses to the environment, and has been shown to control transcription-independent rapid responses occurring in seconds as well as slow transcriptional responses [1][2][3][4][5][6][7] . The latter requires the intracellular receptors TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX proteins (AFBs) that promote the degradation of their co-receptors AUX/IAA transcriptional repressors and act as adenylate cyclases [8][9][10][11][12][13] . Increasing studies show that many auxin-induced effects such as plasma-membrane hyperpolarization, cytosolic Ca 2+ -transients, activation of ROP GTPases, RAF-like kinase-dependent cytoplasmic streaming, and protoplast swelling, are too rapid to depend on transcriptional regulation 5,7,[14][15][16][17][18][19][20][21] .…”