Adenosine deaminase in body fluids: A useful diagnostic tool in tuberculosis

Segura, Ramón; Pascual, Carlos Bardají; Ocaña, I; Martínez-Vázquez, J M; Ribera, Esteban; Ruíz, Isabel; Pelegrí, M.D.

doi:10.1016/s0009-9120(89)80013-x

Cited by 73 publications

(31 citation statements)

References 25 publications

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“…In one report, high pleural £uid ADAwas found in all of nine patients with rheumatoid pleurisy and in ten of 15 patients with lymphoma. 23 Recently, it has been suggested that the test performance of ADA isoenzymes is superior to ADA, yielding lower false negative and false positive ¢ndings. 40,48,50 Finally, limitations of our study should be noted.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of adenosine deaminase in tuberculous pleural effusion: a meta-analysis

et al. 2003

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Section: Discussionmentioning

confidence: 99%

Diagnostic value of adenosine deaminase in tuberculous pleural effusion: a meta-analysis

et al. 2003

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“…Each value is a mean of duplicate determinations. because the Mycobaterium bacilli are scanty in clinical specimens (24,36,37). Furthermore histological examination is regarded as invasive and Acid Fast Bacilli may be difficult to find on staining.…”

Section: Adenosine Deaminase Assaymentioning

confidence: 99%

“…In recent years, clinical interest in ADA has been focused on the enzyme assay in pleural fluids (22)(23)(24)(25)(26)(27)(28)(29)39). Piras et al (30), were the first to suggest that the measm'ement of ADA activity in pleural fluids could be useful in the assessment of tuberculous patients who are presented with pleural effusions where a significant increase in enzyme activity occurs.…”

Section: Introductionmentioning

confidence: 99%

Adenosine deaminase activity in serum and pleural effusions of tuberculous and non‐tuberculous patients

Al-Shammary

1997

IUBMB Life

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SUMMARYIn a retrospective study Adenosine deaminase (ADA), was assayed in 86 serum samples and 12 pleural fluid samples of patients with pulmonary tuberculosis. Serum and pleural fluid ADA levels were also examined in a group of 38 non-tuberculous patients with pleural effusion. Highly significant increases in both serum and pleural fluid ADA levels were noted in tuberculous patients when compared to both control enzyme cut-off values and non-tuberculous p]eural effusion patients. Negative Ziehl-Nielsen staining for acid fast bacilli and Purified Protein Derivative skin test results were obtained in 32.3% and 11.1% of the examined patients respectively. All these patients showed significantly elevated serum or pleural fluid ADA levels. It is hereby suggested that serum and pleural fluid ADA levels can in conjanction with other tests, serve as a marker in patients of pulmonary tuberculosis.

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“…In this context, attempts have been made to identify markers which allow more rapid and accurate diagnosis. One such marker is adenosine deaminase (ADA), which has been proposed to be a useful diagnostic marker for tuberculous 7 disease in pleura, pericardium, and peritoneum . Several reports have suggested that an elevated pleural fluid ADA level predicts tuberculous pleurisy with a sensitivity of 90 to 100% 8 and a specificity of 89 to 100% .…”

Section: Introductionmentioning

confidence: 99%

Significance of Adenosine Deaminase in Diagnosing Tuberculous Pleural Effusion in Nepalgunj Medical College Teaching Hospital Kohalpur

Shyam

Shah

Pandey

et al. 2017

J. Nepalgunj Med. College

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Background: Tuberculosis (TB) is a major public health problem in developing countries including Nepal. One of the common presentations of TB is pleural effusion. The diagnosis of tubercular pleural effusion can be difficult because of the low rate of detecting tubercular bacilli by direct stain and culture of pleural fluid for acid-fast bacilli (AFB). Pleural biopsy can be useful but is invasive and requires experts. In this context, pleural fluid Adenosine Deaminase (ADA) level has been proposed as easy, cheap and highly sensitive test for diagnosis of TB pleural effusion. Objectives: The present study was undertaken to define the role of pleural fluid ADA value in accurate diagnosis of Tubercular pleural effusion. Methods: A Prospective analysis of 68 patients admitted in Nepalgunj Medical College teaching Hospital was done from January 2014 to December 2015 with pleural effusion. Pleural fluid ADA level was evaluated in all patients, and significance of pleural fluid ADA level in TB pleural effusion was studied. Results: Age of patients were between 20 to 80 years, with the minimum being 20 years and maximum being 79 years. In this study 85% of cases had pleural effusion due to tuberculosis. Out of the 68 patients with pleural effusion, 58(85%) were finally diagnosed to be due to tuberculosis, 2 were diagnosed to be due to malignancy, 4 due to pneumonia leading to parapneumonic effusion, 1 due to congestive heart failure and 3 due to nephrotic syndrome. Conclusion: It is difficult to diagnose TB pleural effusion by other conventional methods, as it has also been shown in our study also. Previous literatures have also mentioned AFB detection rate to be low from pleural fluid sample. Determination of ADA is a cheap and easy test which we now consider in the early routine evaluation of patients with pleural effusions, particularly if diagnosis of tuberculosis is suspected and in places where prevalence of the disease is still high as is in our country. The other method considered for diagnosing TB pleural effusion is pleural biopsy which is invasive blind procedure and requires high expertise as well.

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Adenosine deaminase in body fluids: A useful diagnostic tool in tuberculosis

Cited by 73 publications

References 25 publications

Diagnostic value of adenosine deaminase in tuberculous pleural effusion: a meta-analysis

Diagnostic value of adenosine deaminase in tuberculous pleural effusion: a meta-analysis

Adenosine deaminase activity in serum and pleural effusions of tuberculous and non‐tuberculous patients

Significance of Adenosine Deaminase in Diagnosing Tuberculous Pleural Effusion in Nepalgunj Medical College Teaching Hospital Kohalpur

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