Addressing the question of the effect of RBC storage on clinical outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7)

Steiner, Marie; Assmann, Susan F.; Levy, Jerrold H.; Marshall, John C.; Pulkrabek, Shelley; Sloan, Steven R.; Triulzi, Darrell J.; Stowell, Christopher P.

doi:10.1016/j.transci.2010.05.014

Cited by 112 publications

(112 citation statements)

References 40 publications

(46 reference statements)

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“…This is especially true for certain categories of recipients, such as traumatized, post-operative and critically ill patients. 1 Despite accumulating retrospective evidence of reduced blood viability after the first 2 weeks of storage, 2 definitive results from prospective, randomized, double-blind clinical trials are still lacking 3,4 or inconclusive. These problems are mainly due to the intrinsic statistical limitations of the experimental models 4 or the lack of common methods and shared standards between laboratories.…”

Section: Introductionmentioning

confidence: 99%

Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomics

D’Alessandro¹,

D’Amici²,

Vaglio³

et al. 2011

Haematologica

184

205

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The online version of this article has a Supplementary Appendix. BackgroundResults from recent, highly debated, retrospective studies raised concerns and prompted considerations about further testing the quality of long stored red blood cells from a biochemical standpoint. Design and MethodsWe performed an integrated mass spectrometry-based metabolomics and proteomics timecourse investigation on SAGM-stored red blood cells. In parallel, structural changes during storage were monitored through scanning electron microscopy. ResultsWe detected increased levels of glycolytic metabolites over the first 2 weeks of storage. From day 14 onwards, we observed a significant consumption of all metabolic species, and diversion towards the oxidative phase of the pentose phosphate pathway. These phenomena coincided with the accumulation of reactive oxygen species and markers of oxidation (protein carbonylation and malondialdehyde accumulation) up to day 28. Proteomics evidenced changes at the membrane protein level from day 14 onwards. Changes included fragmentation of membrane structural proteins (spectrin, band 3, band 4.1), membrane accumulation of hemoglobin, antioxidant enzymes (peroxiredoxin-2) and chaperones. While the integrity of red blood cells did not show major deviations at day 14, at day 21 scanning electron microscope images revealed that 50% of the erythrocytes had severely altered shape. We could correlate the scanning electron microscopy observations with the onset of vesiculation, through a proteomics snapshot of the difference in the membrane proteome at day 0 and day 35. We detected proteins involved in vesicle formation and docking to the membrane, such as SNAP alpha. ConclusionsBiochemical and structural parameters did not show significant alterations in the first 2 weeks of storage, but then declined constantly from day 14 onwards. We highlighted several parallelisms between red blood cells stored for a long time and the red blood cells of patients with hereditary spherocytosis.Key words: red blood cell, storage, mass spectrometry, proteomics, metabolomics. Haematologica 2012;97(1):107-115. doi:10.3324/haematol.2011 Citation: D'Alessandro A, D'Amici GM, Vaglio S, and Zolla L. Time-course investigation of SAGM-stored leukocyte-filtered red blood cell concentrates: from metabolism to proteomics.

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Section: Introductionmentioning

confidence: 99%

Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomics

D’Alessandro¹,

D’Amici²,

Vaglio³

et al. 2011

Haematologica

184

205

View full text Add to dashboard Cite

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“…Their results will demonstrate whether long RBC storage time is harmful. [4] FWB is more concentrated than blood products prepared with a 1:1:1 ratio of RBCs, frozen plasma, and platelets, and is the only functional alternative that includes all fractions. Nessen et al [5] evaluated the therapeutic role of additional FWB treatment in patients who required platelet replacement after having received RBCs and FFP.…”

Section: Discussionmentioning

confidence: 99%

Should warm fresh whole blood be first choice in acute massive hemorrhage in emergency conditions?

Kendigelen¹,

Kamalak²,

Abat³

2015

Ulus Travma Acil Cerrahi Derg

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Early management of rapid massive hemorrhage requires early administration of blood products and rapid surgical control of bleeding. Professionals in peripheral hospitals with limited resources often work under conditions similar to those in the military. Described in the present report are 3 cases in which warm fresh whole blood (WFWB) was used in patients with massive bleeding who presented to a peripheral hospital that had no blood products suitable for emergency conditions. Described first is the case of a 16-year-old female patient who underwent emergency cesarean section. The patient had massive bleeding from the uterus due to atony. Her hemoglobin (Hb) dropped to 3.5 g/dL. Six units of WFWB were transfused during surgery. Hemodynamic parameters and complete blood count (CBC) stabilized. She was transferred from the intensive care unit (ICU) to obstetrics on day 2 and was discharged on day 7. Described second is the case of a 35-year-old female patient who also underwent emergency cesarean section, and for whom massive bleeding was due to uterine atony. Hb dropped to 2 g/dL and hematocrit (HCT) to 5.4%. Nine units of WFWB were transfused, after which hemodynamic and laboratory parameters stabilized. The patient was extubated the following day, transferred from the ICU to obstetrics on day 3, and was discharged on day 8. Described third is the case of a 36-year-old male patient with stab injuries and hemorrhagic shock who underwent emergency surgery. The patient had injuries to the right renal artery and kidney. Nine units of WFWB were transfused due to continued hemorrhage during surgery. Following surgical control of bleeding and transfusion, hemodynamic parameters improved. The patient was transferred from the ICU on day 5 and discharged on day 10. WFWB transfusion nearly disappeared from civilian medicine after blood was separated into components, and whole blood is not usually available at blood banks. In massive transfusions, WFWB effectively replaces red blood cells (RBCs), platelets, plasma volume, and coagulation factors, while preventing hypothermia and dilutional coagulopathy. Blood components go through biochemical, biomechanical, and immunological changes during long storage, the duration of which affects both transfusion efficacy and associated risks. In the future, with the use of fast donor tests, fast ABO compatibility tests, platelet-sparing leukocyte filters, and developments in pathogen-decreasing technology, fresh whole blood (FWB) may be the first choice for massive transfusion. Future studies will reveal new procedures.

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“…From (28)- (30) approximations for all terms in (27) are easily obtained. This yields a simple approach to the objective function that can be used for a tentative optimization.…”

Section: Approximation Assumptionmentioning

confidence: 99%

Recycled incomplete identification procedures for blood screening

Bar‐Lev

Boxma

Kleiner

et al. 2017

European Journal of Operational Research

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The operation of blood bank systems is characterized by two crucial factors: testing procedures and perishability. We propose a new testing procedure that we term Recycled Incomplete Identification Procedure (RIIP). In RIIP, groups of pooled blood units which are found contaminated in a so-called ELISA test are divided into smaller subgroups and again group-tested by ELISA, and so forth, until finally a so-called PCR test is conducted for those subgroups which are found clean. We analyze and optimize the performance of RIIP, maximizing the profit associated with the procedure. Our numerical results suggest that it may indeed be profitable to do several cycles at ELISA.

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Addressing the question of the effect of RBC storage on clinical outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7)

Cited by 112 publications

References 40 publications

Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomics

Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomics

Should warm fresh whole blood be first choice in acute massive hemorrhage in emergency conditions?

Recycled incomplete identification procedures for blood screening

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