Adaptive protection against damage of preconditioning human umbilical cord-derived mesenchymal stem cells with hydrogen peroxide

Li, D; Xu, Yunze; Gao, Chuanyu; Zhai, Yaping

doi:10.4238/2014.february.21.9

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Since one of the main reasons why MSCs die following engraftment is ROS-mediated oxidative stress [24], several studies have already focused on the beneficial effect of preconditioning cells with sub-lethal doses of H 2 O 2 [1-100 μM]. For instance, preconditioned human umbilical cord MSCs were found to be more resistant to oxidative damage induced by high-concentration H 2 O 2 exposure than controls, which showed a significantly lower cell number than the preconditioned group [33]. H 2 O 2 preconditioning also protected rat bone marrow MSCs against in vitro apoptosis [34].…”

Section: Discussionmentioning

confidence: 99%

H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation

et al. 2020

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Background: Mesenchymal stem cells, including those derived from human adipose tissue (hASCs), are currently being widely investigated for cell therapy. However, when transplanted at the site of injury, the survival and engraftment rates of hASCs are low, mainly due to the harsh microenvironment they encounter, characterized by inflammation and oxidative stress. To overcome these therapeutic limitations, cell preconditioning with lowconcentration of hydrogen peroxide (H 2 O 2) has been proposed as a plausible strategy to increase their survival and adaptation to oxidative stress. Nonetheless, the underlying mechanisms of this approach are not yet fully understood. In this study, we analyzed molecular and bioenergetic changes that take place in H 2 O 2 preconditioned hASCs. Methods: Long-term exposure to a low concentration of H 2 O 2 was applied to obtain preconditioned hASCs (named HC016), and then, their response to oxidative stress was analyzed. The effect of preconditioning on the expression of Nrf2 and its downstream antioxidant enzymes (HO-1, SOD-1, GPx-1, and CAT), and of NF-κB and its related inflammatory proteins (COX-2 and IL-1β), were examined by Western blot. Finally, the Seahorse XF96 Flux analysis system was used to evaluate the mitochondrial respiration and glycolytic function, along with the total ATP production. Results: We found that under oxidative conditions, HC016 cells increased the survival by (i) decreasing intracellular ROS levels through the overexpression of the transcription factor Nrf2 and its related antioxidant enzymes HO-1, SOD-1, GPx-1, and CAT; (ii) reducing the secretion of pro-inflammatory molecules COX-2 and IL-1β through the attenuation of the expression of NF-κB; and (iii) increasing the total ATP production rate through the adaption of their metabolism to meet the energetic demand required to survive. Conclusions: H 2 O 2 preconditioning enhances hASC survival under oxidative stress conditions by stimulating their antioxidant response and bioenergetic adaptation. Therefore, this preconditioning strategy might be considered an excellent tool for strengthening the resistance of hASCs to harmful oxidative stress.

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Section: Discussionmentioning

confidence: 99%

H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation

et al. 2020

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“…Then, since (i) low extracellular Mg induces the synthesis of ROS in various cell types [ 41 ], and (ii) treatment with low concentrations of H 2 O 2 fortifies MSCs against oxidative damage [ 42 ], we evaluated ROS production in AD- and BM-MSCs. We found an increased accumulation of ROS only in BM-MSCs cultured in low vs. their controls in physiologic Mg.…”

Section: Discussionmentioning

confidence: 99%

Magnesium Deprivation Potentiates Human Mesenchymal Stem Cell Transcriptional Remodeling

Sargenti

Castiglioni

Olivi

et al. 2018

IJMS

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Magnesium plays a pivotal role in energy metabolism and in the control of cell growth. While magnesium deprivation clearly shapes the behavior of normal and neoplastic cells, little is known on the role of this element in cell differentiation. Here we show that magnesium deficiency increases the transcription of multipotency markers and tissue-specific transcription factors in human adipose-derived mesenchymal stem cells exposed to a mixture of natural molecules, i.e., hyaluronic, butyric and retinoid acids, which tunes differentiation. We also demonstrate that magnesium deficiency accelerates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. We argue that magnesium deprivation generates a stressful condition that modulates stem cell plasticity and differentiation potential. These studies indicate that it is possible to remodel transcription in mesenchymal stem cells by lowering extracellular magnesium without the need for genetic manipulation, thus offering new hints for regenerative medicine applications.

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“…Aged erythrocytes could be also considered for future study about the impact of aging on their function, monitored through SO 4 = transport measurement, related to hemoglobin modification [ 11 , 48 ]. The putative adaptation of erythrocytes, known as pre-conditioning response to a more or less prolonged oxidative stress [ 49 ], would be also a noteworthy issue.…”

Section: Discussionmentioning

confidence: 99%

H2O2-Induced Oxidative Stress Affects SO4= Transport in Human Erythrocytes

Morabito

Romano²,

Spada

et al. 2016

PLoS ONE

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The aim of the present investigation was to verify the effect of H2O2-induced oxidative stress on SO4= uptake through Band 3 protein, responsible for Cl-/HCO3- as well as for cell membrane deformability, due to its cross link with cytoskeletal proteins. The role of cytoplasmic proteins binding to Band 3 protein has been also considered by assaying H2O2 effects on hemoglobin-free resealed ghosts of erythrocytes. Oxidative conditions were induced by 30 min exposure of human erythrocytes to different H2O2 concentrations (10 to 300 μM), with or without GSH (glutathione, 2 mM) or curcumin (10 μM), compounds with proved antioxidant properties. Since SO4= influx through Band 3 protein is slower and better controllable than Cl- or HCO3- exchange, the rate constant for SO4= uptake was measured to prove anion transport efficiency, while MDA (malondialdehyde) levels and –SH groups were estimated to quantify the effect of oxidative stress. H2O2 induced a significant decrease in rate constant for SO4= uptake at both 100 and 300 μM H2O2. This reduction, observed in erythrocytes but not in resealed ghosts and associated to increase in neither MDA levels nor in –SH groups, was impaired by both curcumin and GSH, whereas only curcumin effectively restored H2O2-induced changes in erythrocytes shape. Our results show that: i) 30 min exposure to 300 μM H2O2 reduced SO4= uptake in human erythrocytes; ii) oxidative damage was revealed by the reduction in rate constant for SO4= uptake, but not by MDA or –SH groups levels; iii) the damage was produced via cytoplasmic components which cross link with Band 3 protein; iv) the natural antioxidant curcumin may be useful in protecting erythrocytes from oxidative injury; v) SO4= uptake through Band 3 protein may be reasonably suggested as a tool to monitor erythrocytes function under oxidative conditions possibly deriving from alcohol consumption, use of drugs, radiographic contrast media administration, hyperglicemia or neurodegenerative diseases.

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Adaptive protection against damage of preconditioning human umbilical cord-derived mesenchymal stem cells with hydrogen peroxide

Cited by 14 publications

References 18 publications

H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation

H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation

Magnesium Deprivation Potentiates Human Mesenchymal Stem Cell Transcriptional Remodeling

H2O2-Induced Oxidative Stress Affects SO4= Transport in Human Erythrocytes

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