Adaptation of a commercial ELISA for the detection of antibodies against Neospora caninum in bovine milk

Schares, Gereon; Bärwald, Andrea; Staubach, Christoph; Wurm, R.; Rauser, M.; Conraths, Franz J.; Schroeder, Carsten

doi:10.1016/j.vetpar.2003.11.016

Cited by 44 publications

(36 citation statements)

References 13 publications

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“…Supondo-se que os níveis de anticorpos no leite refletem os níveis de anticorpos no soro sanguíneo (SCHARES et al, 2004), vacas no início da lactação têm níveis intermediários de anticorpos combinados com alta produção de leite, enquanto vacas que estão produzindo baixos volumes diários de leite apresentam concentração de anticorpos mais alta no leite. SANCHEZ et al (2002) anticorpos encontrados em amostras coletivas de leite estão associados à baixa produção diária de leite.…”

Section: Resultsunclassified

Reação de imunofluorescência indireta para detecção de anticorpos anti-Neospora caninum em amostras coletivas de leite

et al. 2011

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Section: Resultsunclassified

Reação de imunofluorescência indireta para detecção de anticorpos anti-Neospora caninum em amostras coletivas de leite

et al. 2011

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“…Manufacturer's instructions were followed for serum and for milk, but with an exception for the Idx ELISA: milk was diluted 1:2 in the dilution buffer delivered with the ELISA kit, as per recommendation of Schares et al (2004). For Idv ELISA, milk was diluted 1:2 as per manufacturer's recommendations.…”

Section: Antibody Analysesmentioning

confidence: 99%

“…The antibodies found in milk are selectively transported from the serum into the mammary gland and the IgG is the primary immunoglobulin class for bovine milk (Hurley and Theil 2011). ELISA values for milk are linearly correlated to serum ELISA values (Schares et al 2004, González-Warleta et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…Testing of milk samples presents some advantages over testing of blood, like easily and lower costs of collecting samples, noninvasiveness of the method and reduction of stressrelated production loss (Schares et al 2004). When bulk tank milk samples are used, collecting samples is even easier and *Corresponding author: ionitamvet@yahoo.com Violeta Enachescu et al 6 is an economical way of estimating N. caninum prevalence in cattle herds (Frossling et al 2006;Wapenaar et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies conducted in different regions of the world several ELISAs were adapted for detection of Neospora caninum antibodies in cattle using individual milk samples (Björkman et al 1996, Schares et al 2004, Hall et al 2006, bulk milk samples (Bartels et al 2005, Frossling et al 2006, Wapenaar et al 2007 or both (González-Warleta et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in southern Romania

Enăchescu

Ioniţă

Mitrea

2014

Acta Parasitologica

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The study aimed to assess the within-herd Neospora caninum exposure in dairy cattle in southern Romania, based on the detection of specific antibodies in milk and serum. A total of 104 paired samples of milk and serum were collected from four dairy farms. Individual samples were analyzed for N. caninum antibodies by ELISA: IDEXX Neospora Ab (Idx) (three farms: A, B, C; n = 60) and ID-VET Lab (Idv) (farm D; n = 44). Additionally, four pooled milk samples, one per each farm (A, B, C) and a composed one (A+B+C), were analyzed with Idx ELISA. Optimized cut-off values for milk samples were determined by receiver operating characteristic (ROC) analysis, with serum results considered as true status. The agreement was expressed by K values. The overall seroprevalence of N. caninum infection was 45% in the farms tested by Idx ELISA and 56.8% in the farm tested by Idv ELISA. A good agreement between serum and milk was obtained for both ELISA kits (K = 0.72 and 0.77, respectively). The specificity and sensitivity at optimized cut-off of S/P>0.704 for Idx and S/P%>7.966% for Idv were 100% and 70.37% for Idx and 89.47% and 88% for Idv. Testing pooled milk samples, there were identified as N. caninum positive the dairy farms with a 15% or higher within-herd seroprevalence at the cut-off value of S/P>0.51. This is the first study in Romania in which milk samples were tested to determine the N. caninum infection status in dairy farms, providing a base for further researches.

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The first detection of Neospora caninum DNA in the colostrum of infected cows

et al. 2006

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Limited data is available on the vertical transmission of Neospora caninum via the colostrum. The results of our previous research revealed the presence of N. caninum DNA in the milk of seropositive cows. The aim of the present work is to demonstrate parasite DNA in colostrum samples. A polymerase chain reaction using Np21 and Np6 primers was applied to DNA isolated from the colostrum sediment in order to amplify the corresponding genomic Nc-5 region. The expected 328-bp product was obtained in colostrum samples collected both on the calving day and the day after. This is the first detection of N. caninum DNA in the colostrum of seropositive cows, and these findings implicate the possibility of N. caninum transmission through the colostrum.

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Adaptation of a commercial ELISA for the detection of antibodies against Neospora caninum in bovine milk

Cited by 44 publications

References 13 publications

Reação de imunofluorescência indireta para detecção de anticorpos anti-Neospora caninum em amostras coletivas de leite

Reação de imunofluorescência indireta para detecção de anticorpos anti-Neospora caninum em amostras coletivas de leite

Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in southern Romania

The first detection of Neospora caninum DNA in the colostrum of infected cows

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