Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions

Zhang, Xinfu; An, Xinmin

doi:10.3389/fmicb.2022.876174

Cited by 4 publications

(3 citation statements)

References 127 publications

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“…The Cas14 protein ( Figure 1 D) is a low-molecular-weight (400–700 amino acid) RNA-guided nuclease that recognizes target ssDNA and cleaves it without restriction sequences [ 88 , 89 ]. CRISPR/Cas14 also performs non-specific cleavages around ssDNA nuclease molecule like CRISPR/Cas12 [ 88 ].…”

Section: Crispr/cas 14 Systemsmentioning

confidence: 99%

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Wang

et al. 2022

Diagnostics

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The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown excellent performance in the development of pathogen nucleic-acid-detection technology. In combination with various biosensing technologies, researchers have made many rapid, convenient, and feasible innovations in pathogen nucleic-acid-detection technology. With an in-depth understanding and development of the CRISPR/Cas system, it is no longer limited to CRISPR/Cas9, CRISPR/Cas12, and other systems that had been widely used in the past; other CRISPR/Cas families are designed for nucleic acid detection. We summarized the application of CRISPR/Cas-related technology in infectious-disease detection and its development in SARS-CoV-2 detection.

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Section: Crispr/cas 14 Systemsmentioning

confidence: 99%

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Wang

et al. 2022

Diagnostics

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“…Cas14 protein, also referred to as Cas12f, is the shortest member of the CRISPR-associated protein family, consisting of approximately 400–700 amino acids ( Zhang and An, 2022 ). There are three subtypes of Cas14: Cas14a, Cas14b, and Cas14c.…”

Section: Detection Of Pathogen Nucleic Acid Based On Trans Cleavage A...mentioning

confidence: 99%

Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid

Li,

Zhong,

et al. 2023

Front. Mol. Biosci.

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Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are widely used as gene editing tools in biology, microbiology, and other fields. CRISPR is composed of highly conserved repetitive sequences and spacer sequences in tandem. The spacer sequence has homology with foreign nucleic acids such as viruses and plasmids; Cas effector proteins have endonucleases, and become a hotspot in the field of molecular diagnosis because they recognize and cut specific DNA or RNA sequences. Researchers have developed many diagnostic platforms with high sensitivity, high specificity, and low cost by using Cas proteins (Cas9, Cas12, Cas13, Cas14, etc.) in combination with signal amplification and transformation technologies (fluorescence method, lateral flow technology, etc.), providing a new way for rapid detection of pathogen nucleic acid. This paper introduces the biological mechanism and classification of CRISPR-Cas technology, summarizes the existing rapid detection technology for pathogen nucleic acid based on the trans cleavage activity of Cas, describes its characteristics, functions, and application scenarios, and prospects the future application of this technology.

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“…When exogenous DNA enters bacteria or archaea that employ the CRISPR-Cas system, the CRISPR-associated protein complex in the host binds to protospacer adjacent motifs (PAM) of the exogenous DNA, and a DNA sequence adjacent to the PAM is used as a candidate protospacer sequence. Then, under the action of related proteins, the protospacer sequence is integrated between the LS and the first repeat sequence to form a new spacer sequence ( Zhang and An, 2022 ). The sequence information from the exogenous DNA that is stored between the repeats then serves as an immunological memory ( Bhaya et al, 2011 ).…”

Section: Summary Of the Crispr-cas Systemmentioning

confidence: 99%

CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

Huang

Fang

Zhou³

et al. 2022

Front. Microbiol.

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Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms. The review aims to provide insights to spur further development by summarizing the characteristics of effectors of the CRISPR-Cas13 system and by describing the latest research into its application in the rapid detection of pathogenic microorganisms in combination with nucleic acid extraction, isothermal amplification, and product detection.

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Adaptation by Type III CRISPR-Cas Systems: Breakthrough Findings and Open Questions

Cited by 4 publications

References 127 publications

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid

CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

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