Acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of a cellulosic exopolysaccharide obtained from sugarcane molasses

Abstract

The acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of BC were studied. Cytotoxicity of BC was evaluated in cultured C3A hepatoma cells (HepG2/C3A) using a lactate dehydrogenase (LDH) activity assay. Acute toxicity was tested in adults Wistar rats treated with a single dose of BC. The genotoxicity of BC was evaluated in vivo by the micronucleus assay. BC (0.33-170 μg/mL) added to C3A cell culture medium caused no elevation in LDH release over the background level recorded in untreated cell wells. The treatment with the BC in a single oral dose (2000 mg/kg body weight) caused no deaths or signs of toxicity. BC attenuated CP-induced and inhibition the incidence of MNPCE (female: 46.94%; male: 22.7%) and increased the ratio of PCE/NCE (female: 46.10%; male: 35.25%). There was no alteration in the LDH release in the wells where C3A cells were treated with increasing concentrations of BC compared to the wells where the cells received the cell culture medium only (background of approximately 20% cell death), indicated that in the dose range tested BC was not cytotoxic. BC was not cytotoxic, genotoxic or acutely toxic. BC attenuated CP-induced genotoxic and myelotoxic effects.

A wet dressing for male genital surgery: A phase II clinical trial

“…BC is a biomaterial, obtained by bacterial synthesis from sugarcane molasses (13), described in previous studies as a safe dressing with low toxicity (14), biocompatible (15), promoting growth and cell differentiation (17) and, thus, contributing to the healing process (9, 16, 17) and epithelialization (18). Preclinical and clinical studies have shown that this biomaterial is effective as a mechanical barrier and adjuvant in the treatment of surgical wounds (9) and in ulcerative injuries (17).…”

Efficacy of bacterial cellulose membrane for the treatment of lower limbs chronic varicose ulcers: a randomized and controlled trial

“…Several studies, Including experimental analyzes and clinical trials have shown that BC is non-toxic, biocompatible and effective for tissue remodeling [11][12][13][14] .…”

A new material to prevent urethral damage after implantation of artificial devices: an experimental study

“…In the present study, we used a cellulosic matrix, that has already been evaluated in biomechanical testing and biocompatibility as well as in cytotoxicity assays as a urethral reinforcing wrap in rats by evaluating its integration and remodeling to the host tissue (13, 18, 19). One of the main questions that we consider of interest in this evaluation is the change in the urethral wall layers thickness.…”

“…In this regard, bacterial cellulose (BC), a biomaterial, has demonstrated efficacy as a driver cell and inductor in healing process (11, 12). Studies have proven its safety and efficacy in experimental and clinical models (11-13). …”