Acute exposure of female hamsters to carbendazim (MBC) during meiosis results in aneuploid oocytes with subsequent arrest of embryonic cleavage and implantation

Jeffay, Susan; Libbus, Bisharah L.; Barbee, Randy R.; Perreault, Sally D.

doi:10.1016/0890-6238(96)00020-2

Cited by 43 publications

(11 citation statements)

References 16 publications

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“…In contrast, studies in rodents suggest that both meiosis I and II may be equally sensitive to the aneugenic activities of chemicals (e.g. Mailhes et al 1994Mailhes et al , 1997Zuelke and Perreault 1995;Marchetti et al 1996;Jeffay et al 1996). It is not known whether some chemicals may preferentially induce an error at first or meiosis II.…”

Section: Introductionmentioning

confidence: 81%

Trichlorfon exposure, spindle aberrations and nondisjunction in mammalian oocytes

et al. 1998

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Consumption of trichlorfon-poisoned fish by women in a small Hungarian village has been associated with trisomy resulting from an error of meiosis II in oogenesis. We therefore examined mouse oocytes exposed for 3 h during fertilization to 50 microg/ml trichlorfon. Spindle morphology was not visibly altered by the pesticide. Chromosomes segregated normally at anaphase II with no induction of aneuploidy. However, formation of a spindle was disturbed in many oocytes resuming meiosis I in the presence of trichlorfon. In spite of the spindle aberrations and the failure of bivalents to align properly at the equator, oocytes did not become meiotically arrested but progressed to metaphase II. At this stage, spindles were highly abnormal, and chromosomes were often totally unaligned, unattached or dispersed on the elongated and disorganized spindle. By causing spindle aberrations and influencing chromosome congression, trichlorfon appears, therefore, to predispose mammalian oocytes to random chromosome segregation, especially when they undergo a first division and develop to metaphase II during exposure. This is the first case in which environmentally induced human trisomy can be correlated with spindle aberrations induced by chemical exposure. Our observations suggest that oocytes may not possess a checkpoint sensing displacement of chromosomes from the equator at meiosis I and may therefore be prone to nondisjunction.

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Section: Introductionmentioning

confidence: 81%

Trichlorfon exposure, spindle aberrations and nondisjunction in mammalian oocytes

et al. 1998

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“…Those using newer techniques of antikinetichore antibodies and fluorescence in situ hybridization (FISH) have provided conclusive evidence of the lack of direct interaction with DNA and the existence of thresholds for the aneugenic effects of benomyl and carbendazim (Bentley et al, 2000;Bjorge et al, 1996;Elhajouji et al, 1995;Elhajouji et al, 1997;Jeffay et al, 1996;Mailhes and Aardema, 1992;Sarrif et al, 1994b;WHO, 1993a, b). Those using newer techniques of antikinetichore antibodies and fluorescence in situ hybridization (FISH) have provided conclusive evidence of the lack of direct interaction with DNA and the existence of thresholds for the aneugenic effects of benomyl and carbendazim (Bentley et al, 2000;Bjorge et al, 1996;Elhajouji et al, 1995;Elhajouji et al, 1997;Jeffay et al, 1996;Mailhes and Aardema, 1992;Sarrif et al, 1994b;WHO, 1993a, b).…”

Section: Genotoxicitymentioning

confidence: 99%

Inhibitors of DNA Biosynthesis-Mitosis: Benzimidazoles—The Benzimidazole Fungicides Benomyl and Carbendazim

Mull

Hershberger

2001

Handbook of Pesticide Toxicology

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“…Treatment with carbendazim has also induced aneuploidy in hamster oocytes (Jeffay et al, 1996) and diploidy in rat epididymal sperm in a dose dependent fashion (de Stoppelaar et al, 1999).…”

Section: Genotoxicity Of Pesticidesmentioning

confidence: 99%

Pesticides and the induction of aneuploidy in human sperm

Härkönen¹

2005

Cytogenet Genome Res

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Pesticides are some of the most frequently released toxic chemicals into the environment. Exposure to them has been associated with reproductive dysfunction, but the knowledge of the genotoxic risks of these substances is still limited. In vitro and in vivo, many pesticides are shown to induce aneuploidy. Analysis of sperm chromosomes by fluorescence in situ hybridization (FISH) with chromosome-specific probes has obtained increasing popularity in genetic toxicology. Sperm-FISH studies on men exposed to pesticides have yielded conflicting results: in men exposed to multiple pesticides during spraying no increased disomy frequencies in sperm were observed, although one study reported an increased rate of sex chromosome nullisomy. In contrast the two studies conducted in pesticide factories showed increased frequencies of sperm aneuploidy in exposed men compared to controls. The available data indicates that at least some of the commonly used pesticides are capable of inducing aneuploidy in human sperm when the exposure level is high enough.

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Acute exposure of female hamsters to carbendazim (MBC) during meiosis results in aneuploid oocytes with subsequent arrest of embryonic cleavage and implantation

Cited by 43 publications

References 16 publications

Trichlorfon exposure, spindle aberrations and nondisjunction in mammalian oocytes

Trichlorfon exposure, spindle aberrations and nondisjunction in mammalian oocytes

Inhibitors of DNA Biosynthesis-Mitosis: Benzimidazoles—The Benzimidazole Fungicides Benomyl and Carbendazim

Pesticides and the induction of aneuploidy in human sperm

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