Acute ethanol exposure induces [Ca²⁺]_i transients, cell swelling and transformation of actin cytoskeleton in astroglial primary cultures

Abstract

] i transients in a small population of the cells. Cell swelling was estimated using a new method based on three-dimensional¯uorescence imaging in conjunction with image analysis and graphic visualization techniques. The method provides detailed results concerning the reformation of structural shape and speci®c volume alterations, as well as total proportions between the different states. Astroglial cell swelling was registered and quanti®ed in 7 of 39 cells chosen from 12 different coverslips. EtOH also induced reversible conformational changes in ®lamentous actin, appearing as increases in ring formations and a more dispersed appearance of the ®laments. Filamentous actin was stained with Alexa phalloidin after incubation with EtOH for varied periods. The results presented here suggest that EtOH affects astrocytes in a way that could be of physiological relevance.

Naloxone and Ouabain in Ultralow Concentrations Restore Na+/K+-ATPase and Cytoskeleton in Lipopolysaccharide-treated Astrocytes

“…Others have seen morphological changes induced by LPS in cultured astrocytes (48,49). In line with earlier published data, the present findings confirm that there is a connection between rearrangement of actin filaments and changes in astrocytic morphology and cell volume (46,47).…”

“…We found that the actin filaments were disorganized after many h of LPS exposure. The actin filaments, which are normally organized in stress fibers, changed into ring structures after 1 h of treatment and were disrupted and disorganized at 24 h. We have observed similar patterns previously when astrocytes were exposed to ethanol, ammonium chloride, or lactate (46,47). Others have seen morphological changes induced by LPS in cultured astrocytes (48,49).…”

Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells — Comparison with effects of CCK

“…We concluded this from results in nonpancreatic cells showing that ethanol influences PKC subcellular distribution and activity [21][22][23][24][25] and that it induces transformations of the actin cytoskeleton [26][27][28]. However, to our knowledge, pancreatic acinar cells have not yet been tested for such effects of ethanol.…”

Proteomic and metabolomic analysis on the toxicological effects of As (III) and As (V) in juvenile mussel Mytilus galloprovincialis

“…Actin plays critical roles in shaping and maintaining cell morphology, as well as in supporting various cellular functions, including cell motility, cell division, and intra-cellular transport (Allansson et al, 2001). Physiological stress could lead to increasing number of actin-containing stress fibers and change the characteristic of the growth-arrested state of cells (Thomas et al, 1981).…”