Acute and sub-acute oral toxicity studies of standardized extract of Nasturtium officinale in Wistar rats

Clemente, Mirna; Miguel, Marílis Dallarmi; Gribner, Caroline; Moura, Paula Francislaine; Rigoni, Annamaria; Fernandes, Luiz C.; Carvalho, J. L. S.; Hartmann, Ingridy Fhadine; Piltz, Marina Talamini; Henneberg, Raílson; Montrucchio, Deise Prehs; Miguel, Obdúlio Gomes

doi:10.1016/j.yrtph.2019.104443

Cited by 13 publications

(15 citation statements)

References 21 publications

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“…However, abrupt variations in the body weight indicate the toxic nature of the chemicals or drugs (Dongmo et al, 2019). In this study, LA treatment and control group recorded a normal gain in body weight, indicating, LA does not cause any physiological alterations that affects the normal growth in the animals, which is in agreement with the previous study published by Clemente et al (2019). Similarly, organ weight variation is the fundamental and perceptive factor correlating organ to body weight index, which is important and critical in determining drug toxicity linked to treatment-related effects (Sellers et al, 2007).…”

Section: Discussionsupporting

confidence: 92%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

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Background Lauric acid (LA), a common constituent of coconut oil, is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods The study was conducted in accordance with the Organization for Economic Co-operation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2,000 mg/kg body weight, respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results The study results showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion The current finding suggests that single oral administration of LA, even up to 2,000 mg/kg body weight, did not exhibit any signs of toxicity in SD rats; thus, it was safe to be used on disease models in animals.

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Section: Discussionsupporting

confidence: 92%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

View full text Add to dashboard Cite

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“…However, abrupt variations in the body weight indicate the toxic nature of the chemicals or drugs (Dongmo et al 2019). In this study, LA treatment and control group recorded a normal gain in body weight, indicating, LA does not cause any physiological alterations that affects the normal growth in the animals, which is in agreement with the previous study published by (Clemente et al 2019). Similarly, organ weight variation is the fundamental and perceptive factor correlating PeerJ reviewing PDF | (2019:11:43420:1:2:NEW 12 Feb 2020)…”

Section: Discussionsupporting

confidence: 92%

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Sari¹

2020

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Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

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“…The preparation of the final extract (Hydro‐glycolic extract) to be used in the preclinical study (Clemente, Miguel, Felipe, et al, 2019; Clemente, Miguel, Gribner, et al, 2019) and this clinical trial, was with the content of 5.0 mg/ml of phenylethyl glucosinolate, following (Anvisa, 2011; Brasil, 2014).…”

Section: Methodsmentioning

confidence: 99%

“…Details of the extraction, High-Performance liquid chromatography (HPLC) analysis, and preparation of the final formulation has been followed (de Souza Carvalho, 2008) and validated for selectivity, linearity, accuracy, robustness intra-day and inter-day precisions, according After achievement of HPLC chromatogram and spectrogram of this fluid extract of watercress, its chemical constituents were identified with its content, adapted to the (de Souza Carvalho, Cunico, de Fátima Gaspari Dias, Miguel, & Miguel, 2009) methodology and (Clemente, Miguel, Felipe, et al, 2019;) study ( Table 1).…”

Section: High-performance Liquid Chromatography Analysismentioning

confidence: 99%

“…Nasturtium officinale R. Br (watercress) is a perennial dicotyledonous cruciferous vegetable usually found close to the water and contains flavonoids, phenolic acids, glucosinolates, and isothiocyanate compounds, with potential effect as an anti‐inflammatory agent. And in regular diet, this plant has demonstrated protective effects on human health against oxidative stress (Canistro et al, 2004; Clemente, Miguel, Felipe, et al, 2019; Clemente, Miguel, Gribner, et al, 2019; Manchali, Chidambara Murthy, & Patil, 2012; Sadeghi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Biomarkers of oxidative stress and inflammation in people witha physical disability treated with a standardized extract ofNasturtium officinale: A randomized, double‐blind, and placebo‐controlled trial

Clemente

Miguel

Gribner

et al. 2020

Phytotherapy Research

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It is well established that plants from the Brassicaceae family, particularly watercress, have been associated to reduce oxidative DNA damage. Nasturtium officinale R. Br (watercress) contains glucosinolates, with anti-inflammatory action and protective effect on human health against oxidative stress. We aimed to evaluate whether the standardized extract of Nasturtium officinale (SENO) is capable of changing biomarkers of oxidative stress and inflammation in people with physical disabilities. 65 people enrolled this study: as a control group composed by; 15 people with no physical disability assessed once, 25 people with physical disabilities using 750 mg/kg/day of SENO, and 25 people with physical disabilities using 750 mg/kg/day of placebo-control for 5 weeks. Biomarkers of oxidative stress and inflammation were analyzed on day 0 and 36. The results indicated that SENO was associated with decreasing levels of lipid peroxidation, protein carbonyl, catalase, superoxide dismutase, and C-reactive protein. Furthermore, the cytokine kit demonstrated below and out of invertible range, which was impossible to detect the inflammatory process. Despite the cytokine kit was not able to detect the inflammation; these data might provide supportive evidence that SENO, have affected positively people with physical disabilities decreasing their biomarkers of oxidative stress and C-reactive protein. Further studies are required.

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Acute and sub-acute oral toxicity studies of standardized extract of Nasturtium officinale in Wistar rats

Cited by 13 publications

References 21 publications

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Biomarkers of oxidative stress and inflammation in people witha physical disability treated with a standardized extract ofNasturtium officinale: A randomized, double‐blind, and placebo‐controlled trial

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