Acute and chronic toxicological studies of Dimorphandra mollis in experimental animals

Feres, Cássia A. O.; Madalosso, R.C.; Rocha, Orivaldo A.; Leite, João Paulo Viana; Guimarães, Thiago Teixeira; Toledo, V.P.P.; Tagliati, Carlos Alberto

doi:10.1016/j.jep.2006.06.002

Cited by 62 publications

(41 citation statements)

References 34 publications

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“…The fruits of this species represent an important therapeutic source, especially as a venous tonic and antioxidant, providing an alternative for the prevention and treatment of circulatory problems. A pre-clinical toxicity study demonstrated to be low for this extract (Féres et al, 2006). No alterations in the levels of total protein, albumin and globulin were observed in any of the groups evaluated (Figure 1).…”

Section: Discussionmentioning

confidence: 85%

“…This immunotoxicological study of D. mollis was conducted with doses of 1000 and 2000 mg/kg for thirty days. The doses used in this study were obtained from LD50 test results (Féres et al, 2006). It is known that a yield of 76.0±3% of rutin is obtained from the DMDE obtained from D. mollis fruits (Féres et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The doses used in this study were obtained from LD50 test results (Féres et al, 2006). It is known that a yield of 76.0±3% of rutin is obtained from the DMDE obtained from D. mollis fruits (Féres et al, 2006). The fruits of this species represent an important therapeutic source, especially as a venous tonic and antioxidant, providing an alternative for the prevention and treatment of circulatory problems.…”

Section: Discussionmentioning

confidence: 99%

“…The dried, semi-purified D. mollis extract (DMDE) was considered to be non-classified in the preclinical studies of toxicity, according to the European Community, and presented no toxicity in the pharmacological screening (Féres et al, 2006). However, the DMDE has not been extensively studied, although it is known that it is effective in clinical processes and is not toxic.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Feres¹,

Toledo²,

Tagliati³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Avaliação dos efetos imunotoxicológicos de Dimorphandra mollis Benth., Fabaceae, em ratos". A Dimorphandra mollis Benth., Fabaceae, conhecida como faveira ou favad´anta, é uma planta comum do cerrado central do Brasil, muito utilizada por suas propriedades antioxidante, antiplaquetária e, principalmente, como vasoprotetora. Seu principal marcador é a rutina. Este estudo teve como objetivo avaliar a segurança da utilização do extrato seco de D. mollis em roedores. O extrato foi extraído, padronizado e quantificado apresentando teor de 76,0±3% de rutina. Nos parâmetros bioquímicos e hematológicos avaliados, não se observou alterações nos grupos de machos e fêmeas que receberam a dose de 1000 e 2000 mg/kg de D. mollis, mas observou-se um aumento de eosinófilos. Nos estudos histopatológicos detectou-se hiperreatividade da polpa branca esplênica, no grupo que recebeu a dose de 2000 mg/kg de D. mollis. Na avaliação da resposta linfoproliferativa, com 1000 e 2000 mg/kg não foram observadas alterações, e somente nos estudos com a dose de 2000 mg/kg se observou diminuição de IgG. Os resultados obtidos, utilizando roedores, sugerem que nenhuma toxicidade existe na administração de extrato seco de D. mollis na dose de 1000 mg/kg. Unitermos: Dimorphandra mollis, rutina, imunotoxicologia, roedores.ABSTRACT: Dimorphandra mollis Benth., Fabaceae, also known as "faveira" or "fava-d´anta", is a plant common to the central woodsy meadow region of Brazil. It is well known for its antioxidant, antiplatelet and, principally, vasoprotective properties. Its principal component is rutin. The objective of this study is the evaluation of the safety of the use of the dried D. mollis extract in rodents. The rutin content of the standardized extract was 76.0±3%. With respect to the biochemical and hematological parameters evaluated, no alterations in the groups of rats that received 1000 and 2000 mg/kg doses of D. mollis were observed, but an increase in eosinophiles occurred. Hyperactivity of the white splenic pulp was detected in the group that received the 2000 mg/kg dose of D. mollis. In the evaluation of the lymphproliferative response with 1000 and 2000 mg/kg, no alterations were observed, and a decrease in IgG was only observed in the studies with a 2000 mg/kg dose. The results obtained with rodents suggest that no toxicity exists with the administration of dried D. mollis extract in a 1000 mg/kg dose.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Feres¹,

Toledo²,

Tagliati³

et al. 2010

Rev. bras. farmacogn.

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“…D. mollis is a medicinal plant of high economic value that has been extensively exploited in recent years, mainly because its fruits contain the flavonoid rutin. Rutin has antioxidant, antiviral, antitumoral, and anti-inflammatory properties (Féres et al, 2006), and is widely used to produce medications for human circulatory diseases (Gomes and Gomes, 2000). Studies indicate that this species also has potential for use in the food industry because its seeds contain a high amount of galactomannan, which can be used as a thickener, stabilizer, and gelling agent (Panegassi et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Isolation of high quality and polysaccharide-free DNA from leaves of Dimorphandra mollis (Leguminosae), a tree from the Brazilian Cerrado

Souza¹,

Müller²,

Brandão³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Dimorphandra mollis (Leguminosae), known as faveiro and fava d'anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications for human circulatory diseases. Knowledge about its genetic diversity is needed to guide decisions about the conservation and rational use of this species in order to maintain its diversity. DNA extraction is an essential step for obtaining good results in a molecular analysis. However, DNA isolation from plants is usually compromised by excessive contamination by secondary metabolites. DNA extraction of D. mollis, mainly from mature leaves, results in a highly viscous mass that is difficult to handle and use in techniques that require pure DNA. We tested four protocols for plant DNA extraction that can be used to minimize problems such as contamination by polysaccharides, which is more pronounced in material from mature leaves. The protocol that produced the best DNA quality initially utilizes a sorbitol buffer to remove mucilaginous polysaccharides. The macerated leaf material is washed with this buffer until there is no visible mucilage in the sample. This protocol is adequate Isolation of high-quality DNA of Dimorphandra mollis for DNA extraction both from young and mature leaves, and could be useful not only for D. mollis but also for other species that have high levels of polysaccharide contamination during the extraction process.

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Toxicity Protocols for Natural Products in the Drug Development Process

Beressa

Annu

Mtewa

2020

Poisonous Plants and Phytochemicals in Drug Discovery

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Acute and chronic toxicological studies of Dimorphandra mollis in experimental animals

Cited by 62 publications

References 34 publications

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Evaluation of the immunotoxicological effects of Dimorphandra mollis Benth., Fabaceae, in rats

Isolation of high quality and polysaccharide-free DNA from leaves of Dimorphandra mollis (Leguminosae), a tree from the Brazilian Cerrado

Toxicity Protocols for Natural Products in the Drug Development Process

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