Actual ratios of triacylglycerol positional isomers consisting of saturated and highly unsaturated fatty acids in fishes and marine mammals

Analysis of regioisomers of polyunsaturated triacylglycerols in marine matrices by HPLC/HRMS

“…This signal intensity discrepancy between thermally and collisionally induced fragmentations was not recognised in previous published works concerning the regioisomers characterisation of FAs probably due to the fact that there was uniformity of TAGs constituents (low-medium chain FAs with relatively low unsaturation degree like palmitic, oleic, linoleic, linolenic, myristic and stearic acids) in these studies (Brydwell et al, 2002;Gotoh et al, 2011;.…”

“…Many articles illustrate the characterisation of different oils (Brydwell & Neff, 2002;Mottram & Evershed, 1996, 2001Dugo, Beccaria, Fawzy, Donato, & Cacciola, 2012;Gotoh et al, 2011;Lévêque, Héron, & Tchapla, 2010;Mottram, Woodbury, & Evershed, 1997;Mottram, Crossman, & Evershed, 2001;Nagy, Sandoz, Destaillats, & Schafer, 2013;Nagy et al, 2013;Neff, Brydwell, & List, 2001).…”

Actual Ratios of Triacylglycerol Positional Isomers and Enantiomers Comprising Saturated Fatty Acids and Highly Unsaturated Fatty Acids in Fishes and Marine Mammals

“…The actual ratios of sn-PPD/PDP/sn-DPP, sn-PPE/PEP/sn-EPP, sn-DDP/DPD/sn-PDD, and sn-EEP/ EPE/sn-PEE in marine organisms are summarized in Tables 2 and 3. As has been previously reported 13 , marine mammals do not contain PDP and PEP, which is in contrast to fishes species, which contain TAGs with HUFA bound at the sn-2 position. However, the trends in the actual ratios of sn-PPD/sn-DPP and sn-PPE/sn-EPP in marine mammals and fishes are the same, with HUFA mainly bound at the sn-3 position.…”

“…We recently reported the actual ratio of TAG positional isomers in marine organisms consisting of HUFAs such as DHA and EPA and saturated fatty acids such as palmitic acid. These were analyzed using HPLC-atmospheric pressure chemical ionization APCI /mass spectrometry MS equipped with a polymeric ODS column 13 . We assumed that the actual ratios of the TAG positional isomers consisting of palmitic acid, EPA, and DHA partially represented the characteristics of TAGs bound to HUFA in marine organisms.…”

Regiospecific Analysis of Shark Liver Triacylglycerols

“…Only 3.2 % of DHA and 4.6 % of EPA were esterified to the sn-2 position in harp seal oil [18]. Further, in seal blubber [19] and whale oils [20] DHA occurred mainly in the sn-1 and sn-3 positions. These studies show that shark liver oil has more or less similar characteristics compared to other fish oils that are presently used as DHA supplements or nutraceuticals in the pharmaceutical industry.…”