Actual Ratio of Triacylglycerol Positional Isomers in Milk and Cheese

Abundances of Triacylglycerol Positional Isomers and Enantiomers Comprised of a Dipalmitoylglycerol Backbone and Short- or Medium-chain Fatty Acids in Bovine Milk Fat

“…The result that only β-PPC 4 was detected in BMF was consistent with our previous paper 19 . The detected ratio of β-PC 10 P/β-PPC 10 conflicts with our previous report 20 . The reason for this is unclear, but this inconsistency might be caused by differences in the origin and season in which the milk was produced 25,26 .…”

“…Although we have already reported that the ratio of β-PC 10 P/β-PPC 10 in the milk and cheese of ruminants including cow 20 , the data indicated only the ratio of β-PC 10 P/ β-PPC 10 but not their amounts in milk fat. Meanwhile, we have already quantified β-PC 4 P/β-PPC 4 in BMF 19 .…”

Simple method for the quantification of milk fat content in foods by LC-APCI-MS/MS using 1,2-dipalmitoyl-3-butyroyl-glycerol as an indicator

“…In fact, the AOCS official method for the quantification of milk fat in chocolate using GC-FID uses this idea. We also selected PPBu as the targeted TAG in milk fat, because the separation of these TAG isomers using the method we developed previously was very simple 16,17 . We used rac-PPBu to prepare the calibration curve; the PPBu that originally exists in milk fat is not racemic because the amounts of the two enantiomers, namely sn-PPBu and sn-BuPP, are not the same.…”

“…Recently, we developed a simple method for the separation of TAG positional isomers using LC-APCI-MS/MS with an octacocyl silylation C28 column. This method can separate TAG positional isomers consisting of two long-chain saturated fatty acids and one short-chain saturated fatty acid, which are characteristically contained in milk fat 16,17 .…”

Quantification of Triacylglycerol Positional Isomers in Rat Milk

“…The procedure to obtain rat milk samples were the same as in our previous study 20 . Briefly, the mother rats were separated from their neonatal rats and were subcutaneously injected with 2 I.U.…”