Activity of Chemically Synthesized Peptide Encoded by the miR156A Precursor and Conserved in the Brassicaceae Family Plants

Erokhina, Tatiana N.; Ryazantsev, D. Yu.; Samokhvalova, L. V.; Можаев, А. А.; Orsa, Alexander N.; Завриев, С. К.; Morozov, Sergey Y.

doi:10.1134/s0006297921050047

Cited by 10 publications

(16 citation statements)

References 49 publications

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“…In another study, Ormancey et al (2020) also found that FAM (5‐Carboxyfluorescein)‐miPEP165a failed to enter the central cylinder of Arabidopsis within 48 h of applying the labelled peptide. However, it was found that fluorescently labelled miPEP156a from cabbage could efficiently penetrate the root central cylinder and migrate from the roots to the leaves after 18 h of application (Erokhina et al, 2021). The variations in the penetration capacity of miPEPs may impact their functionality in plants.…”

Section: Resultsmentioning

confidence: 99%

“…The external application of miPEPs from other plant species, such as Medicago (miPEP171b), cabbage (miPEP156a), and grapevine (mi-PEP171d1), also led to higher accumulation of their corresponding miRNAs and modulated phenotypes (Chen et al, 2020;Erokhina et al, 2021;Lauressergues et al, 2015), suggesting a common mode of action of miPEPs in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the exogenous application of synthetic miPEP858a modulated flavonoid biosynthesis and development in Arabidopsis as a result of the enhanced abundance of miR858a (Sharma et al, 2020). The external application of miPEPs from other plant species, such as Medicago (miPEP171b), cabbage (miPEP156a), and grapevine (miPEP171d1), also led to higher accumulation of their corresponding miRNAs and modulated phenotypes (Chen et al, 2020; Erokhina et al, 2021; Lauressergues et al, 2015), suggesting a common mode of action of miPEPs in plants.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‐encoded regulatory peptides modulate cadmium tolerance and accumulation in rice

Lu,

Chen,

Chen

et al. 2024

Plant Cell & Environment

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MicroRNAs (miRNAs) are small noncoding RNAs that play a vital role in plant responses to abiotic and biotic stresses. Recently, it has been discovered that some primary miRNAs (pri‐miRNAs) encode regulatory short peptides called miPEPs. However, the presence of miPEPs in rice, and their functions in response to abiotic stresses, particularly stress induced by heavy metals, remain poorly understood. Here, we identified a functional small peptide (miPEP156e) encoded by pri‐miR156e that regulates the expression of miR156 and its target SPL genes, thereby affecting miR156‐mediated cadmium (Cd) tolerance in rice. Overexpression of miPEP156e led to decreased uptake and accumulation of Cd and reactive oxygen species (ROS) levels in plants under Cd stress, resulting in improved rice Cd tolerance, as observed in miR156‐overexpressing lines. Conversely, miPEP156e mutants displayed sensitivity to Cd stress due to the elevated accumulation of Cd and ROS. Transcriptome analysis further revealed that miPEP156e improved rice Cd tolerance by modulating Cd transporter genes and ROS scavenging genes. Our study provides insights into the regulatory mechanism of miPEP156e in rice response to Cd stress and demonstrates the potential of miPEPs as an effective tool for improving crop abiotic stress tolerance.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MicroRNA‐encoded regulatory peptides modulate cadmium tolerance and accumulation in rice

Lu,

Chen,

Chen

et al. 2024

Plant Cell & Environment

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“…Particularly, chemically synthesized At-miPEP156a (33 amino acids in length), being the highly conservative among miPEPs of Brassicaceae [ 66 ], can significantly stimulate transcription of pri-miR156a in Brassica oleraceae and Brassica rapa [ 55 ] ( Table 1 ). We have previously revealed that two last species also encode miPEPs (Bo-miPEP156a and Br-miPEP156a), through which exogenous application to Brassica seedlings increases the main root length [ 61 ]. Importantly, chemically synthesized Bo-miPEP156a stimulates the transcription of pri-miR156a in A. thaliana [ 55 ].…”

Section: Possible Effects Of Lncrna-encoded Peptides On Plant Physiol...mentioning

confidence: 99%

Biological Activity of Artificial Plant Peptides Corresponding to the Translational Products of Small ORFs in Primary miRNAs and Other Long “Non-Coding” RNAs

Erokhina,

Ryazantsev,

Zavriev

et al. 2024

Plants

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Generally, lncPEPs (peptides encoded by long non-coding RNAs) have been identified in many plant species of several families and in some animal species. Importantly, molecular mechanisms of the miPEPs (peptides encoded by primary microRNAs, pri-miRNAs) are often poorly understood in different flowering plants. Requirement for the additional studies in these directions is highlighted by alternative findings concerning positive regulation of pri-miRNA/miRNA expression by synthetic miPEPs in plants. Further extensive studies are also needed to understand the full set of their roles in eukaryotic organisms. This review mainly aims to consider the available data on the regulatory functions of the synthetic miPEPs. Studies of chemically synthesized miPEPs and analyzing the fine molecular mechanisms of their functional activities are reviewed. Brief description of the studies to identify lncORFs (open reading frames of long non-coding RNAs) and the encoded protein products is also provided.

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“…In a very recent finding, using a fluorescein-labeled peptide, it was demonstrated that exogenously applied miPEP-156a could effectively penetrate plant seedlings through the root system and disperse systemically to the leaves of young seedlings of Brassica rapa (Erokhina et al, 2021). The application of this miPEP exerted a moderate morphological effect consisting of a growth acceleration of the main root of the seedling, that was parallel to an increase in pri-miR156a expression.…”

Section: Introductionmentioning

confidence: 98%

Exogenous Application of Non-mature miRNA-Encoded miPEP164c Inhibits Proanthocyanidin Synthesis and Stimulates Anthocyanin Accumulation in Grape Berry Cells

et al. 2021

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Secondary metabolic pathways in grape berries are tightly regulated by an array of molecular mechanisms, including microRNA-mediated post-transcriptional regulation. As recently discovered, before being processed into mature microRNAs (miRNAs), the primary transcripts of miRNAs (pri-miRNAs) can encode for small miRNA-encoded peptides (micropeptides – miPEPs) that ultimately lead to an accentuated downregulation of the respective miRNA-targeted genes. Although few studies about miPEPs are available, the discovery of miPEPs reveals a new layer of gene regulation at the post-transcriptional level that opens the possibility to regulate plant metabolism without resorting to gene manipulation. Here, we identified a miPEP encoded in non-mature miR164c putatively targeting grapevine transcription factor VvMYBPA1 (miPEP164c/miPEP-MYBPA1), a positive regulator of key genes in the proanthocyanidin (PA)-biosynthetic pathway, a pathway that competes directly for substrate with the anthocyanin-biosynthetic pathway. Thus, the objective of this work was to test the hypothesis that the exogenous application of miPEP164c (miPEP-MYBPA1) can modulate the secondary metabolism of grape berry cells by inhibiting PA biosynthetic pathway while simultaneously stimulating anthocyanin synthesis. The exogenous application of miPEP164c to suspension-cultured cells from grape berry (cv. Gamay) enhanced the transcription of its corresponding non-mature miR164c, with a maximum effect at 1 μM and after a period of 10 days, thus leading to a more pronounced post-transcriptional silencing of its target VvMYBPA1. This led to a significant inhibition of the PA pathway, mostly via inhibition of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR) enzymatic activities and VvLAR1 downregulation. In parallel, the anthocyanin-biosynthetic route was stimulated. Anthocyanin content was 31% higher in miPEP164c-treated cells, in agreement with the observed upregulation of VvUFGT1 transcripts and UFGT enzyme activity levels.

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Activity of Chemically Synthesized Peptide Encoded by the miR156A Precursor and Conserved in the Brassicaceae Family Plants

Cited by 10 publications

References 49 publications

MicroRNA‐encoded regulatory peptides modulate cadmium tolerance and accumulation in rice

MicroRNA‐encoded regulatory peptides modulate cadmium tolerance and accumulation in rice

Biological Activity of Artificial Plant Peptides Corresponding to the Translational Products of Small ORFs in Primary miRNAs and Other Long “Non-Coding” RNAs

Exogenous Application of Non-mature miRNA-Encoded miPEP164c Inhibits Proanthocyanidin Synthesis and Stimulates Anthocyanin Accumulation in Grape Berry Cells

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