Activity-dependent pH shifts in hippocampal slices from normal and carbonic anhydrase II-deficient mice

Carbonic anhydrase IV and XIV knockout mice: Roles of the respective carbonic anhydrases in buffering the extracellular space in brain

“…Later studies (5-7) in brain slices showed similar effects with CA inhibitors that do not easily cross membranes, implicating an extracellular CA activity in this buffering. The same effects were observed in the CA II-deficient mouse, ruling out the possibility that the high activity isozyme, CA II, which is very abundant in cytosol of oligodendrocytes and also present in astrocytes, was mediating this effect after its release during preparation of the brain slices (8).…”

Carbonic anhydrase IV and XIV knockout mice: Roles of the respective carbonic anhydrases in buffering the extracellular space in brain

“…Later studies (5-7) in brain slices showed similar effects with CA inhibitors that do not easily cross membranes, implicating an extracellular CA activity in this buffering. The same effects were observed in the CA II-deficient mouse, ruling out the possibility that the high activity isozyme, CA II, which is very abundant in cytosol of oligodendrocytes and also present in astrocytes, was mediating this effect after its release during preparation of the brain slices (8).…”

Imaging of Carbonic Anhydrase Level in Epilepsy with an Environment-Sensitive Fluorescent Probe

“…As mentioned in the literature, CAs were believed to be an important participant in the pathogenesis of epilepsy, which is upregulated in seizures. 62,63 These results indicated that FMRs-CA can visually detect CAs in the PC12 cell damage model and reveal that endogenous CA changes with the dose and time of PTZ stimulation.…”

Regulation and Modulation of pH in the Brain

“…Additional evidence that simple spillage of the enzyme could not account for interstitial CA came from a study of hippocampal slices prepared from mutant mice lacking CA II. In a comparison of mutant versus wild-type animals, activity-dependent interstitial alkaline shifts were comparable in amplitude and were amplified in a similar manner by benzolamide (311). Moreover, histochemical studies in brains of CA II-deficient mice detected the presence of membrane-associated CA (255) near neuronal processes.…”

Carbonic anhydrase IV and XIV knockout mice: Roles of the respective carbonic anhydrases in buffering the extracellular space in brain

“…Later studies (5-7) in brain slices showed similar effects with CA inhibitors that do not easily cross membranes, implicating an extracellular CA activity in this buffering. The same effects were observed in the CA II-deficient mouse, ruling out the possibility that the high activity isozyme, CA II, which is very abundant in cytosol of oligodendrocytes and also present in astrocytes, was mediating this effect after its release during preparation of the brain slices (8).…”

Imaging of Carbonic Anhydrase Level in Epilepsy with an Environment-Sensitive Fluorescent Probe

“…As mentioned in the literature, CAs were believed to be an important participant in the pathogenesis of epilepsy, which is upregulated in seizures. 62,63 These results indicated that FMRs-CA can visually detect CAs in the PC12 cell damage model and reveal that endogenous CA changes with the dose and time of PTZ stimulation.…”

Regulation and Modulation of pH in the Brain

“…Additional evidence that simple spillage of the enzyme could not account for interstitial CA came from a study of hippocampal slices prepared from mutant mice lacking CA II. In a comparison of mutant versus wild-type animals, activity-dependent interstitial alkaline shifts were comparable in amplitude and were amplified in a similar manner by benzolamide (311). Moreover, histochemical studies in brains of CA II-deficient mice detected the presence of membrane-associated CA (255) near neuronal processes.…”

Carbonic anhydrase IV and XIV knockout mice: Roles of the respective carbonic anhydrases in buffering the extracellular space in brain

“…Later studies (5-7) in brain slices showed similar effects with CA inhibitors that do not easily cross membranes, implicating an extracellular CA activity in this buffering. The same effects were observed in the CA II-deficient mouse, ruling out the possibility that the high activity isozyme, CA II, which is very abundant in cytosol of oligodendrocytes and also present in astrocytes, was mediating this effect after its release during preparation of the brain slices (8).…”

Imaging of Carbonic Anhydrase Level in Epilepsy with an Environment-Sensitive Fluorescent Probe

“…As mentioned in the literature, CAs were believed to be an important participant in the pathogenesis of epilepsy, which is upregulated in seizures. 62,63 These results indicated that FMRs-CA can visually detect CAs in the PC12 cell damage model and reveal that endogenous CA changes with the dose and time of PTZ stimulation.…”

Regulation and Modulation of pH in the Brain

“…Additional evidence that simple spillage of the enzyme could not account for interstitial CA came from a study of hippocampal slices prepared from mutant mice lacking CA II. In a comparison of mutant versus wild-type animals, activity-dependent interstitial alkaline shifts were comparable in amplitude and were amplified in a similar manner by benzolamide (311). Moreover, histochemical studies in brains of CA II-deficient mice detected the presence of membrane-associated CA (255) near neuronal processes.…”