Activity and isoenzyme composition of peroxidase and esterase in fertile and male-sterile lines of tomatoes (Lycopersicon esculentum Mill.)

Markova, M.; Ancheva, M.; Atanasova, Biljana

doi:10.1007/bf02923585

Cited by 4 publications

(4 citation statements)

References 13 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It would be difficult to comment on the causes that might determine higher callus frequency in the abnormal anthers, as numerous, and various events are known to cause the genetic anther defects (Kaul, 1988;Markova et al, 1992Markova et al, , 1994Chaudhury, 1993). For this reason, we do not conclude that stamens and/or pollen abnormalities in principle would enhance callus formation.…”

Section: Resultsmentioning

confidence: 69%

Callus induction and plant regeneration via anther culture in mutant tomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

Shtereva¹,

Atanassova²

2001

Israel Journal of Plant Sciences

View full text Add to dashboard Cite

Anthers from two tomato mutants, solanifolia (sf) and trifoliate (tf), characterized by anther abnormalities, 4 male sterile tomato mutants (ms 15 26 , ms 30, ms 32, and ms 33), and their isogenic fertile lines were cultured in vitro, using three modifications of MS media. The frequency of callus induction in the mutant anthers was significantly higher than in the wild type ones in all genotypes, a result independent of the medium. Pseudofruits surrounded by well-developed calli appeared from the anthers of San Marzano ms 15, Van's Early ms 26, San Marzano ms 30, and VF 11 Pearson ms 33. The higher callus induction in the mutant anthers correlated with higher frequency of organogenesis.The fact that, in addition to the ms-mutants, solanifolia and trifoliate also affected callus frequency, suggested that in tomato, process/es leading to, or resulting from anther abnormalities might be favorable for enhancing anther callus induction and subsequent regeneration.Abbreviations: GA 3 -gibberellic acid; IAA-indole-3-acetic acid; IBA-indole-3-butyric acid; IL/NIL-isogenic/near isogenic line; ms-male sterility; MS-Murashige and Skoog medium; NAA-1-naphthaleneacetic acid; sf-solanifolia; tf-trifoliate; 2 iP-6-(γ, γ-dimethylallylamino)purine

show abstract

Section: Resultsmentioning

confidence: 69%

Callus induction and plant regeneration via anther culture in mutant tomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

Shtereva¹,

Atanassova²

2001

Israel Journal of Plant Sciences

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have reported that Class III peroxidases are integral to plant growth, development, and physiological processes, especially for pollen production and stress responses. For example, in a study on male sterility in tomatoes, higher overall peroxidase activity was observed in MS stamens compared with that in male fertile stamens (Markova et al, 1992). Chen et al cloned the Ghpod gene, which contained a conserved domain of Class III peroxidase, and found its expression in the reproductive organs of cotton, particularly pollen, suggesting the involvement of peroxidases in the male reproductive process of angiosperms (Chen et al, 2009).…”

Section: A B Figurementioning

confidence: 99%

Key candidate genes for male sterility in peppers unveiled via transcriptomic and proteomic analyses

Yang,

Luo,

Jin

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

This study aimed to enhance the use of male sterility in pepper to select superior hybrid generations. Transcriptomic and proteomic analyses of fertile line 1933A and nucleic male sterility line 1933B of Capsicum annuum L. were performed to identify male sterility-related proteins and genes. The phylogenetic tree, physical and chemical characteristics, gene structure characteristics, collinearity and expression characteristics of candidate genes were analyzed. The study identified 2,357 differentially expressed genes, of which 1,145 and 229 were enriched in the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases, respectively. A total of 7,628 quantifiable proteins were identified and 29 important proteins and genes were identified. It is worth noting that the existence of CaPRX genes has been found in both proteomics and transcriptomics, and 3 CaPRX genes have been identified through association analysis. A total of 66 CaPRX genes have been identified at the genome level, which are divided into 13 subfamilies, all containing typical CaPRX gene conformal domains. It is unevenly distributed across 12 chromosomes (including the virtual chromosome Chr00). Salt stress and co-expression analysis show that male sterility genes are expressed to varying degrees, and multiple transcription factors are co-expressed with CaPRXs, suggesting that they are involved in the induction of pepper salt stress. The study findings provide a theoretical foundation for genetic breeding by identifying genes, metabolic pathways, and molecular mechanisms involved in male sterility in pepper.

show abstract

“…After that the plants were planted in pots outside to study the phase of IV th leaf and bud-formation. Enzyme extracts preparation were previously described (Markova et al 1992). Electrophoretic resolution was carried out by the method of vertical block electrophoresis in 7.5 % polyacrylamide gel (PAAG) and a continu- …”

Section: Methodsmentioning

confidence: 99%

Alcohol dehydrogenase loci of sterile and fertile lines of tomato in the process of ontogenesis

2000

View full text Add to dashboard Cite

A biochemical analysis through electrophoresis in polyacrylamide gel of alcohol dehydrogenase loci in three ontogenetic phases of tomatoes' development was carried out. This subject of investigation were the male-sterile line 6944 aa and the fertile anthocyan line 6944 aa +. Higher levels of isozyme expression in leaves in the cotyledon phase of anthocyan plants, particularly of isozyme No 1 in the Adh-2 locus, was found. The connection between alcohol dehydrogenase, and pollen male sterility and anthocyan staining (aa +) were discussed. A quantitative molecular marker connected to a genetic marker (in this case) for the purposes of identifying sterile from fertile plants in the earliest phase of their development was established.List of abbreviations: ADH -alcohol dehydrogenase, aa -lack of anthocyan, aa + -presence of anthocyan, EST -esterase, ms -male sterility, PAAGpolyacrylamide gel

show abstract

Activity and isoenzyme composition of peroxidase and esterase in fertile and male-sterile lines of tomatoes (Lycopersicon esculentum Mill.)

Abstract: Activity and isoenzyme composition of peroxidase and esterase in fertile and male.sterile lines of tomatoes (Lycopersicon esculentum Mill.

Cited by 4 publications

References 13 publications

Callus induction and plant regeneration via anther culture in mutant tomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

Callus induction and plant regeneration via anther culture in mutant tomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

Key candidate genes for male sterility in peppers unveiled via transcriptomic and proteomic analyses

Alcohol dehydrogenase loci of sterile and fertile lines of tomato in the process of ontogenesis

Contact Info

Product

Resources

About