Activités biologiques des extraits aqueux de Pergularia tomentosa L. (Asclepiadaceae)

Cherif, R.; Kemassi, Abdellah; Boual, Zakaria; Bouziane, N.; Benbrahim, Fouzi; Hadj-Seyd, A.; Gharib, Toufik; Hadj-Khelil, Aminata Ould El; Sakeur, M.L.; Hadj, Mohamed Didi Ould El

doi:10.22453/lsj-017.1.025035

Cited by 15 publications

(10 citation statements)

References 12 publications

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“…Previous studies reported the presence of the ghalakinoside, calactin, uzarigenin, and pergularoside within the roots and desglucouzarin, coroglaucigenin, and uzarigenin together with α-sitosterol glycoside in the leaves (Alsaid et al, 1988;Gohar et al, 2000). According to numerous reports, P. tomentosa leaves are a rich source of flavonoids and cardenolides it can be a good source of natural antioxidant, antibacterial, cytotoxic, and allelopathic compounds (Cherif et al, 2016;Hosseini Kahnouj et al 2017;Lahmar et al, 2017). Despite P. tomentosa has several traditional uses and various biological effects, very little information is available concerning their effects on the arthropod fauna and only few studies refer to the insecticidal activities of this plant (Acheuk and Doumandji-Mitiche 2013;Paul et al, 2011).…”

Section: Evaluation Of Cytotoxic Effects Of Methanolic Extract Of Permentioning

confidence: 99%

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

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Alshammari

2021

Asian Pac J Cancer Prev

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Background: Pergularia tomentosa is a member of the Apocynaceae family found in a wide geographical region including the Gulf region, North Africa and the Middle East. It is known as Fattaka, Ghalqa or Am Lebina in Saudi Arabia, It is used as a remedy for the treatment of skin sores, asthma, and bronchitis. Objective: This study aims to investigate the cytotoxic effects of methanolic extract and Latex (milky secretion) extract. Methods: The stem of Pergularia tomentosa was cut, air dried and soaked for 72 h with methanol repeatedly three times. The crude latex (milk extract) was collected from healthy stem parts of P. tomentosa L by cutting the petiole of leaves, and left to flow where a thick white liquid (Milky) were secreted, collected in amber glass tube and extracted with methanol. Further, the methanolic extract was fractionated by subsequent extraction with various solvents, viz. n-hexane, ethyl acetate and methanol. The cytotoxic effects of Pergularia tomentosa L were evaluated using three cancer cell lines of colon carcinoma (HCT-116), hepatocellular carcinoma (HepG2) and breast carcinoma (MCF-7). The cytotoxic effects of Pergularia tomentosa L extracts against HCT-116, HepG2, and MCF-7 were determined by crystal violet staining method. Results: The potency of plant extract to decrease the cell viability of human cancer cells was arranged in descending order as follows: Methanol extract (IC50 = 10.2 μg/ml, 13.6 μg/ml and 29.5μg/ml, respectively). > Milky secretion extract (IC50 = 52.6 μg/ml, 58.6 μg/ml and 120 µg/ml, respectively). Methanolic extract was strong cytotoxic activity against HCT-116 and HepG2 (IC50= 10.2, 13.6 µg/ml, respectively) and moderately activity against MCF-7 (IC50= 29.5 µg/ml). The Milky extract exhibited moderate activity against HCT-116 and HepG2 (IC50= 52.6-58.6 µg/ ml, respectively) and weak activity against MCF-7 (120.0 µg/ml). Conclusion: The methanol extract of Pergularia tomentosa L showed higher cytotoxic effect as compared to the Latex (Milky secretion) extract. These extracts can be used as natural antitumor. In Future modern chromatographic separations are needed to get more quantity of metabolites. Further detailed investigation of the isolated metabolites is required to identify the phytoconstituents responsible for antioxidant and cytotoxic effects.

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Section: Evaluation Of Cytotoxic Effects Of Methanolic Extract Of Permentioning

confidence: 99%

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

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Alshammari

2021

Asian Pac J Cancer Prev

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“…A seed is germinated when there is the opening of the seed and the emergence of a 3 mm stem. [25] According to Côme, the germination rate (Gr) corresponds to the percentage of germinated seeds compared to the total seed sown, it is estimated by the following formula: [26][27] Gr = (Ng / Ns) × 100 Ng: Number of seeds sprouted. Ns: Number of seeds sown.…”

Section: Germination Rate Of the Seeds Of Lepidium Sativummentioning

confidence: 99%

“…It allows a precise vision of the germination evolution of a seed lot placed under specific conditions. [26][27] Germination inhibition rate (GI%) The ability of an extract or substance to inhibit seed germination is expressed by the following relationship: [24] GI% = (PG control -PG test / PG control) × 100 PG control: Germination percentage of the control lot PG test: Germination percentage of the lot treated by extracts Vigor of seedling Lepidium sativum After determination of germination for seven days for each seed lot, a measure of the length of the radical is performed. This value is expressed as an average of extension of rootlets and the results are noted in mm.…”

Section: Kinetics Of Germinationmentioning

confidence: 99%

“…Germination is a transitional phase where the seed passes from a life slowed to a young autotrophic seedling. It is a very complex biochemical and physiological process that can be measured by several factors such as imbibition and respiration, [27] as well as the ability of a product to inhibit this phase is a complex process. Under natural conditions and in the presence of an exogenous stimulus such as water mainly, the seeds germinate.…”

Section: Effect Of Capparis Spinosa Extracts On the Germination Of Lepidium Sativum Seedsmentioning

confidence: 99%

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Evaluation of the Antioxidant Activity and the Cytotoxicity of Extracts of Capparis Spinosa

Ennacerie

Filali

Moukrad

et al. 2018

Int. J. Pharm. Sci. Drug Res.

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The purpose of this study is to promote the plant spontaneous, harvested from the West of Morocco and streamline its use in quantifying the total phenolic content, assessing the antioxidant activity and testing the degree of toxicity on two types of eukaryotic cells plants and animals. The content, extracts aqueous and ethanolic flower buds and fruit, in phenolic compounds was determined according to the method Follin- Ciocalteu. The antioxidant activity was evaluated by two methods FRAP and DPPH. As for the cytotoxicity extracts, it has been evaluated by the test hemolysis and inhibition germination test of Lepidium sativum seeds. The different extracts of the two organs of this plant, have revealed richness in total polyphenols, especially those of the flower buds, as well as, an antioxidant activity, which is in the same order as that of vitamin C for the aqueous extract of flower buds. The effect hemolytic is shown positive for decoctat of flower buds. The ethanolic extract of fruits displayed an activity antimitotic expressed by the inhibition of elongation and growth seedlings of Lepedium sativum. The decoctat of flower exhibited an effect antigerminatif of moderate intensity which is reversible after rehydration of seed. Capparis Spinosa is a plant of quality pharmaceutical interesting for its activities antioxidant, antimitotic, healing, and for its wealth in phenolics compounds

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“…Selon la FAO et International Water Management Institute (2018) chaque année 4,6 millions de tonnes de pesticides chimiques sont pulvérisés dans l'environnement dans le monde et, pays en développement représentent 25% de l'utilisation mondiale des pesticides dans l'agriculture. Cette situation serait à l'origine de la perturbation de l'équilibre écologique de l'écosystème, de la dégradation et de la destruction de la santé, de la biodiversité et de l'environnement (Chérif et al, 2016). Malgré cette forte sollicitation, les producteurs font toujours face à des bio-agresseurs.…”

Section: Introductionunclassified

Connaissances traditionnelles des plantes locales utilisées contre les bio-agresseurs des cultures et produits agricoles chez le peuple Turka au Burkina Faso

Sourabie¹,

Zerbo²,

Yonli³

et al. 2020

Int. J. Bio. Chem. Sci

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La présente étude a pour objectif de faire l’état des connaissances traditionnelles endogènes relatives aux plantes locales utilisées contre les bio-agresseurs des cultures et des produits agricoles. A travers une série d’enquêtes ethnobotaniques réalisée de 2018 à 2019 dans la région des Cascades au Burkina Faso, 150 personnes appartenant à l’ethnie Turka ont été interviewées. Les informations recherchées en utilisant des entretiens semi-directifs, ont concerné les noms locaux des plantes, les parties utilisées, les domaines d’utilisation et leurs modes d'utilisation. Les données collectées ont permis de recenser 33 espèces de plantes réparties en 30 genres et 19 familles utilisées traditionnellement contre les bio-agresseurs agricoles. Ces plantes interviennent sélectivement dans trois domaines : la répulsion des insectes, l’inhibition des herbes envahissantes et la construction des greniers. Les espèces sont utilisées soit directement ou transformées en poudre ou en cendre. Les feuilles sont plus utilisées que les autres organes. Cassia nigricans est l’espèce la plus utilisée pour son effet répulsif et Parkia biglobosa pour son effet inhibiteur. Des études approfondies sur les propriétés chimiques des potentielles espèces permettront de réduire l’impact des produits chimiques en agriculture contre les bio-agresseurs.Mots clés : Ethnobotanique, diversité végétale, ennemi agricole, secteur sud-soudanien, Afrique de l’ouest English Title:Traditional knowledge of local plants used against pests of crops and agricultural products among the Turka poeple in Burkina Faso The objective of this study is to provide an overview of endogenous traditional knowledge relating to local plants used against pests of crops and agricultural products. Through an ethnobotanical’s surveys carried out from 2018 to 2019 in the Cascades area in Burkina, 150 Turka ethnic group people were interviewed. Through semi-structured interviews, information looking for were concerned plants local names, their parts used, the domains and the modes of use. Data collected allowed to identify 33 plants’ species belonged to 30 genera and 19 families, used traditionally against agricultural pests. These plants are selectively involved in three domains: repelling insects, inhibiting invasive weeds and building lofts. Species are used either directly or processed into powder or ash. The leaves are used more than other organs. Cassia nigricans is the most used species for its repellent effect and Parkia biglobosa for its inhibitory effect. In-depth studies on the chemical properties of potential species will reduce the impact of chemicals in agricultural against pests.Keywords: Ethnobotany, plant diversity, agricultural pest, South Sudanese sector, West Africa

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Activités biologiques des extraits aqueux de Pergularia tomentosa L. (Asclepiadaceae)

Cited by 15 publications

References 12 publications

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA

Evaluation of the Antioxidant Activity and the Cytotoxicity of Extracts of Capparis Spinosa

Connaissances traditionnelles des plantes locales utilisées contre les bio-agresseurs des cultures et produits agricoles chez le peuple Turka au Burkina Faso

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