Activités antibactériennes de l’extrait des feuilles de Dalechampia clematidifolia (Euphorbiaceae)

Tsirinirindravo, L.; Andrianarisoa, Blandine

doi:10.4314/ijbcs.v3i5.51098

Cited by 10 publications

(15 citation statements)

References 2 publications

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“…The aim here was to describe the antimicrobial activity of U. chamae roots and link this activity to chemical compounds. First, the evaluation of the antimicrobial activity of Uvaria chamae root extracts indicated high activity for ethanol extracts according to previously described standard diffusion methods [ 24 , 27 ]. This activity was only measured against Gram-positive cocci and none was visualized against any of the tested Gram-negative strains.…”

Section: Discussionmentioning

confidence: 99%

“…Each activity test was performed three times to ensure results consistency. Culture inhibition diameters were measured and compared to normalized values previously described [ 24 ]. The average of each inhibition diameter obtained was compared with the mean diameters obtained for reference antibiotic discs (Vancomycin and Mupirocin for Staphylococcus spp., Vancomycin for Enterococcus spp., and Colistin and Imipenem for Negative Gram bacilli strains).…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria

Koudokpon

Armstrong

Dougnon

et al. 2018

BioMed Research International

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This study presents antimicrobial properties of Uvaria chamae roots, commonly used for the treatment of various infections in south Benin. Their constituents were extracted and then fractionated in order to isolate the active ingredients. Antimicrobial susceptibility tests were performed against several multidrug-resistant bacteria using the Mueller Hilton well agar diffusion method. Results showed that ethanol extracts were highly active against Gram-positive cocci. This activity was more extensive than that measured from conventional broad-spectrum antibiotics. Indeed, vancomycin-resistant enterococcus (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) strains were all sensitive to this root extract. The aim of this study was to link the antimicrobial activity of the root to chemical structures. The ion mobility mass spectrometry analysis revealed for the first time the presence of ten chalcone and dihydrochalcone structures responsible for the antimicrobial activity of Uvaria chamae ethanol extracts. Two structures were described here for the first time in these roots. These findings confirm and justify the medical properties of these roots used as a traditional medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria

Koudokpon

Armstrong

Dougnon

et al. 2018

BioMed Research International

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“…The DPPH method was carried out by adding 50 μl of the diluted extracts to 1950 μL of DPPH at 130 μM. Discoloration of DPPH was measured at 516 nm against the blank (1950 μl of DPPH at 130 μM and 50 μl of ethanol) after 45 min (Haddadi et al, 2011) Tsirinirindravo and Andrianarisoa (2009). (Haddadi et al, 2011):…”

Section: Antioxidant Activity By Dpph Methodsmentioning

confidence: 99%

Bioactive compounds and antimicrobial potential of the roots extract of Anogeissus leiocarpa, a chewing stick used for oral care in Benin Republic

ThÃ©ophile¹,

Paul²,

̈me³

et al. 2020

J. Pharmacognosy Phytother.

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Chewing stick are still used in developing countries for oral hygiene in other to prevent oral diseases. But still, few is known about their phytochemical potential and antimicrobial activity. The present work was devoted to one of these plants used in the Republic of Benin, namely the root of Anogeissus leiocarpa. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods were used for the antioxidant activity of three crude extracts (aqueous, decoction and ethanolic). Antimicrobial activity of the crude extracts as well as three fractions namely the chloroform fraction, the ethyl acetate fraction and the butanol fraction was carried out by the diffusion method. High performance liquid chromatography (HPLC) analysis of the three fractions of A. leiocarpa was performed to identify the active fraction as well as bioactive compounds. The results show that the crude extracts exhibited a good ability to inhibit the DPPH radical and a good ability to reduce ferric Fe 3+ ions to ferrous Fe 2+ ion and this could be explained by their good content in phenolic compounds. The ethanolic extract of A. leiocarpa was the most active against all microorganisms used in this study. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) ranged from 0.195 to 12,500 mg/mL. The butanolic fraction was the most active with an inhibition diameter of 20.666 ± 0.577 and 22.333 ± 2.081 mm, respectively at the concentration of 50 and 100 mg/mL. HPLC analysis revealed the presence of phenolic acids such as chlorogenic, ferulic and gallic acids as well as tannins including tannic acid and ellagic acid and from these results, A. leiocarpa is a good plant candidate for the production of herbal toothpaste.

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“…The incubated plates were examined to measure the zones of inhibition. The standard used for reading the results of the antibiogram tests is presented in Table 2 (Tsirinirindravo and Andrianarisoa 2010;WHO 2002).…”

Section: • Sensitivity Test Of Bacterial Strains To Extractsmentioning

confidence: 99%

Assessment of the antibacterial effect of Khaya senegalensis on some Gram-negative bacteria

et al. 2021

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Background The matter of antimicrobial resistance evokes the urgency to explore alternatives to the antibiotics traditionally used for microbial infections. This study aimed to elucidate the mechanism of action of the antibacterial effect of Khaya senegalensis liable for bacterial strains responsible for diarrheal infections. Results The data collected indicate that the bacterial strains tested (Salmonella Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Shigella spp. and Salmonella spp.) were sensitive to the extracts of Khaya senegalensis (Desr.) A.Juss. (aqueous and hydro-ethanol) to varying degrees. The hydro-ethanolic extract was active on all strains with a MIC of 25 mg/mL coupled with a bactericidal effect. The aqueous extract was only active on the Salmonella spp. strain. Membrane permeability test data show that Khaya senegalensis extracts affect the bacterial strains tested by attacking the stability of their outer membrane. This potential indicated by the high percentage of membrane destabilization of the bacteria is significantly (p < 0.05) better than that of cefixime used as a reference. Conclusion This study revealed that Khaya senegalensis destroys Gram-bacteria by attacking the stability of their cytoplasmic membrane. These data provide for the first time the mode of action of Khaya senegalensis extracts concerning their antibacterial activity.

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Activités antibactériennes de l’extrait des feuilles de Dalechampia clematidifolia (Euphorbiaceae)

Cited by 10 publications

References 2 publications

Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria

Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria

Bioactive compounds and antimicrobial potential of the roots extract of Anogeissus leiocarpa, a chewing stick used for oral care in Benin Republic

Assessment of the antibacterial effect of Khaya senegalensis on some Gram-negative bacteria

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