Active-oxygen scavenging activity of traditional nourishing-tonic herbal medicines and active constituents of Rhodiola sacra

Ohsugi, Mizue; Fan, Wenzhe; Hase, Koji; Xiong, Quanbo; Tezuka, Yasuhiro; Komatsu, Kenshi; Namba, Tsuneo; Saitoh, Takashi; Tazawa, Kenji; Kadota, Shigetoshi

doi:10.1016/s0378-8741(98)00245-1

Cited by 124 publications

(91 citation statements)

References 12 publications

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“…For each experiment, the effects of all compounds are represented as the percent of the mean control value set at 100%. The scavenging effect is termed as the signal intensity (percent of control) as described previously (18,19).…”

Section: In Vitro Esr Measurementmentioning

confidence: 99%

Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

et al. 2011

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Abstract. In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO • ), superoxide anion radical (O 2•-), and singlet oxygen ( 1 O 2 ), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO• and 1 O 2 . Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 -315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on 1 O 2 was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged 1 O 2 . These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on 1 O 2 . It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

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Section: In Vitro Esr Measurementmentioning

confidence: 99%

Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

et al. 2011

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“…As one of the most important traditional herbal remedies, R. crenulata has long been used in the treatment of long-term illnesses and weaknesses due to infection in Tibet and other regions for more than 1000 years (Xiong, 1995;Zhao et al, 1998;Rohloff, 2002). Recent pharmacological studies have found that this plant contains many ingredients such as salidrosides which are highly active against anoxia, fatigue, toxic reactions, radiation sickness, tumors and aging as well as presenting active-oxygen scavenging properties (Kurkin and Zapesochnaya, 1986;Peng et al, 1996;Ohsugi et al, 1999). Lei et al (2003Lei et al ( , 2004 investigated the interpopulation chemical variability of essential oils from R. crenulata rhizomes from Tibet and Yunnan and found that there were two main chemotypes of the essential oils in Tibetan (geraniol-rich) and Yunnan (n-octanol-and geraniol-rich) R. crenulata populations which can be used for identifying the source of R. crenulata genetic resources.…”

Section: Introductionmentioning

confidence: 99%

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats

et al. 2006

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The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively high (p = 97.83%, and H o = 0.464) and analysis using Shannon's index showed that the within and between genetic diversity of R. crenulata are approximately equal. Nei's genetic distance and unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis showed that the three populations from Tibet and the six populations from Yunnan form two major clusters. The Yunnan populations from three locations were further divided into three corresponding groups, indicating that genetic differentiation was correlated to geographic distribution. Understanding the genetic structure of R. crenulata provides insight for the conservation and management of this endangered species.

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“…Thus, the search for bioactive compounds that can regulate the activities of MMP-1, -2, and -9 from natural resources is one of the key steps in delaying skin aging. Rhodiola rosea L. (Crassulaceae), an herbaceous plant, is used in Asian and Eastern European traditional medicines for its pressure-reducing [4], neurosystem-stimulating [5], fatigue-removing [6], hypobaropathy-preventing [7], antidepressive [7], anticancer [8], antiaging [9], cardiovascular-protective [10], and hepatoprotective effects [11]. Chemical investigations on this plant have revealed many chemical entities, including phenolic acids [12], phenolethanoids [13], phenylpropenoids [14], flavonoids [15], monoterpenes [4], and glycosides [16].…”

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confidence: 99%

Anti-MMP-2 Activity and Skin-Penetrating Capability of the Chemical Constituents from Rhodiola rosea

et al. 2016

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Introduction !Matrix metalloproteinases (MMPs) are a family of zinc-and calcium-dependent endopeptidases, which are grouped into the metzincin clan of metallopeptidases (MPs) together with other families such as ADAMs/adamalysins, astacins, fragilysins, and serralysins. MMPs are found throughout the animal and plant kingdoms, where their distribution is consistent with a Darwinian tree-based pathway [1]. Furthermore, polyplication has led to the presence of several paralogous MMP genes in the same organism: 24 in humans, 26 in sea urchins, 26 in zebrafish, 7 in sea squirts, and 2 in fruit flies. Overexpression of MMPs may cause various inflammatory, malignant, and degenerative symptoms [2]. Among the MMPs studied, gelatinase-A (MMP-2), gelatinase-B (MMP-9), and collagenase-1 (MMP-1) were reported to be responsible for the signal transduction of dermal photoaging [3], and inhibition of the activities of these two enzymes could potentially slow down skin aging. Thus, the search for bioactive compounds that can regulate the activities of MMP-1, -2, and -9 from natural resources is one of the key steps in delaying skin aging. [16]. In a preliminary biological evaluation, crude extracts of R. rosea roots exhibited inhibitory activities toward MMP-2 and collagenase at a concentration of 100 µg/mL [17]. An investigation of the active principles of this plant was thus undertaken by using a bioassayguided method. Of the tested water, n-butanol, and ethyl acetate layers, the ethyl acetate layer Abstract ! Based on the significant inhibitory activity toward matrix metalloproteinase-2 and collagenase noticed in preliminary studies, crude extracts of Rhodiola rosea were partitioned and chromatographed sequentially to afford three new compounds, 1,2,3,6-tetra-O-galloyl-4-O-p-hydroxybenzoyl-β-D-glucopyranoside (1), (E)-creoside I (2), and (R,Z)-2-methylhept-2-ene-1,6-diol (3), along with twenty-four known compounds (4-27). Their structures were determined by spectroscopic data analyses. All isolated compounds were subjected to bioactivity assays. In these, 1 specifically inhibited matrix metalloproteinase-2 activity with an IC 50 value of 16.3 ± 1.6 µM, while its analogue 1,2,3,6-tetra-O-galloyl-β-D-glucopyranonoside (17) inhibited matrix metalloproteinase-2 with an IC 50 value of 23.0 ± 4.8 µM. In the collagenase activity assay, the inhibitory effects of 1 and 17 at concentrations of both 20 and 40 µM were more potent than those of the positive control, 1,10-phenanthroline. In order to realize whether 17 could penetrate from the epidermal layer into the basal and dermal layers of the human skin to inhibit the activity of matrix metalloproteinase-2 and collagenase or not, a transdermal penetration test in nude and white mice skins was performed. Penetration percentages of 17 quantified by LC-MS were 27.8 % and 74.8 % in 24 hours, respectively. Supporting information available online at

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Active-oxygen scavenging activity of traditional nourishing-tonic herbal medicines and active constituents of Rhodiola sacra

Cited by 124 publications

References 12 publications

Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats

Anti-MMP-2 Activity and Skin-Penetrating Capability of the Chemical Constituents from Rhodiola rosea

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