Activation of p53-Mediated Apoptosis Pathway in HSC3 Cancer Cell Irradiated by Atmospheric DBD Oxygen Plasma

Hayashi, Nobuya; Miyamaru, Yukie; Aijima, Reona; Yamashita, Yoshio

doi:10.1109/tps.2018.2867431

Cited by 17 publications

(18 citation statements)

References 33 publications

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“…Reactive species generated in the gas plasma phase can induce endoplasmic reticulum (ER) stress resulting in increased calcium influx into the mitochondria and mitochondria-dependent apoptosis 53 , 54 . Upon plasma-mediated activation of pro-apoptotic signaling cascades, an increase of Bax over Bcl2 55 , the release of mitochondrial cytochrome c 56 , and subsequent activation of p53 have been observed 57 . Alterations in signaling pathways in response to oxidative stress 58 – 60 may impair plasma treatment efficacy to display cytotoxicity in different cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Miebach

Freund

Horn

et al. 2021

Sci Rep

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Recent research indicated the potential of cold physical plasma in cancer therapy. The plethora of plasma-derived reactive oxygen and nitrogen species (ROS/RNS) mediate diverse antitumor effects after eliciting oxidative stress in cancer cells. We aimed at exploiting this principle using a newly designed dual-jet neon plasma source (Vjet) to treat colorectal cancer cells. A treatment time-dependent ROS/RNS generation induced oxidation, growth retardation, and cell death within 3D tumor spheroids were found. In TUM-CAM, a semi in vivo model, the Vjet markedly reduced vascularized tumors' growth, but an increase of tumor cell immunogenicity or uptake by dendritic cells was not observed. By comparison, the argon-driven single jet kINPen, known to mediate anticancer effects in vitro, in vivo, and in patients, generated less ROS/RNS and terminal cell death in spheroids. In the TUM-CAM model, however, the kINPen was equivalently effective and induced a stronger expression of immunogenic cancer cell death (ICD) markers, leading to increased phagocytosis of kINPen but not Vjet plasma-treated tumor cells by dendritic cells. Moreover, the Vjet was characterized according to the requirements of the DIN-SPEC 91315. Our results highlight the plasma device-specific action on cancer cells for evaluating optimal discharges for plasma cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Miebach

Freund

Horn

et al. 2021

Sci Rep

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show abstract

“…Most of these experimental in vitro studies used the oral squamous cell carcinoma cell line SCC-15, as well as SCC-25, and highlighted their plasma-induced inactivation due to the reduction of cell growth, induction of apoptosis, and secondary DNA damage, especially through plasma-derived ROS [19,25,28,[34][35][36][37][38]. Other HNSCC cell lines were also tested, showing one or more of the tumor-toxic plasma-associated characteristics [6,19,22,24,[26][27][28][29][30][31][32][33]37]. In addition, HNSCC has been shown to be more prone to physical plasma treatment than non-malignant cells since the viability of the latter did not significantly decrease after plasma application.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Besides EGFR, studies suggested a crucial role of the p53-signaling pathway in plasma-induced head and neck cancer cell death [23,29,33,37]. This protein contributes to the redox balance within the cells via regulation of the antioxidant gene expression and further senses DNA damage, initiates signaling pathways for the DNA repair, and fulfills the role of a cell-cycle regulator [20].…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Moreover, Hayashi et al and Mine et al demonstrated the contribution of p53 during plasma-induced apoptosis. They showed that plasma-mediated activation of mitogen-activated protein kinase (MAPK) proteins, like c-Jun N-terminal kinase (JNK) and p38, induces p53 phosphorylation, which resulted in apoptosis of the HNSCC cell line HSC3 [29,33].…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…In addition to the different signaling pathways that are induced through the physical plasma application, the redox state of the cancer cell lines is also influenced by the plasma treatment. It has been reported that the antioxidative activity of cancer cells is generally lower than in non-malignant cells due to a compromised redox state, showing decreased levels of the two major redox state regulators glutathione (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) [30,33]. The additional intracellular oxidative stress that is generated by plasma-derived ROS leads to further imbalances in the cellular redox state and the loss of the MMP.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

et al. 2020

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Despite progress in oncotherapy, cancer is still among the deadliest diseases in the Western world, emphasizing the demand for novel treatment avenues. Cold physical plasma has shown antitumor activity in experimental models of, e.g., glioblastoma, colorectal cancer, breast carcinoma, osteosarcoma, bladder cancer, and melanoma in vitro and in vivo. In addition, clinical case reports have demonstrated that physical plasma reduces the microbial contamination of severely infected tumor wounds and ulcerations, as is often seen with head and neck cancer patients. These antimicrobial and antitumor killing properties make physical plasma a promising tool for the treatment of head and neck cancer. Moreover, this type of cancer is easily accessible from the outside, facilitating the possibility of several rounds of topical gas plasma treatment of the same patient. Gas plasma treatment of head and neck cancer induces diverse effects via the deposition of a plethora of reactive oxygen and nitrogen species that mediate redox-biochemical processes, and ultimately, selective cancer cell death. The main advantage of medical gas plasma treatment in oncology is the lack of adverse events and significant side effects compared to other treatment modalities, such as surgical approaches, chemotherapeutics, and radiotherapy, making plasma treatment an attractive strategy for the adjuvant and palliative treatment of head and neck cancer. This review outlines the state of the art and progress in investigating physical plasma as a novel treatment modality in the therapy of head and neck squamous cell carcinoma.

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Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

Rasouli

Mehdian

Hajisharifi

et al. 2021

Plasma Processes & Polymers

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Recently, cold atmospheric pressure plasmas (CAP) have emerged as a promising oncotherapeutic modality, through physical and chemical effects. Here, for the first time, A2780 CP and SKOV-3 cells, relevant to ovarian cancer and GCs as normal ovarian cells were evaluated through CAP directly, indirectly, and concomitant modality of plasma activated medium (PAM) with common drugs to overcome chemotherapy resistance in ovarian cancer. Our results confirm the high potential and the stronger selectivity of PAM in comparison to CAP for the selected cell lines and selectivity mechanism was related to the pH and concentration of H2O2, NO2 -, and NO3reactive species in the plasma stimulated medium. Compared to the combination of common carboplatin (CAR) and paclitaxel (PTX) chemotherapy treatments, the PAM-based treatment is very promising for ovarian cancer treatment. Our data verify that PAM alone and in combination with carboplatin sensitizes cancer cells to carboplatin, inhibits the SOD1 gene, and selectively induces apoptosis accompanied with high expression of p53, Bax, and activation of Caspase-3.

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Activation of p53-Mediated Apoptosis Pathway in HSC3 Cancer Cell Irradiated by Atmospheric DBD Oxygen Plasma

Cited by 17 publications

References 33 publications

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Tumor cytotoxicity and immunogenicity of a novel V-jet neon plasma source compared to the kINPen

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

Plasma‐activated medium induces apoptosis in chemotherapy‐resistant ovarian cancer cells: High selectivity and synergy with carboplatin

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