Activation of Bombyx neuropeptide G protein-coupled receptor A4 via a Gαi-dependent signaling pathway by direct interaction with neuropeptide F from silkworm, Bombyx mori

Deng, Xiaoyan; Yang, Huipeng; He, Xiaobai; Yuan, Ling; Zheng, Congxia; Zhou, Qi; Zhu, Chenggang; Zhang, Guozheng; Gao, Jimin; Zhou, Naiming

doi:10.1016/j.ibmb.2013.12.007

Cited by 25 publications

(25 citation statements)

References 33 publications

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“…Although less research has been performed on the localization and distribution of NPF receptor mRNA than on NPF mRNA, the expression of the NPFR transcript in the aphid head and digestive system is consistent with the localization data obtained for other insect species (Garczynski et al, 2002 ; Nässel and Wegener, 2011 ; Deng et al, 2014 ). For instance, in situ hybridization experiments have revealed abundant NPFR expression in the brain, midgut, and accessory nerve of 3rd instar Drosophila (Garczynski et al, 2002 ).…”

Section: Discussionsupporting

confidence: 85%

“…The NPFR-encoding transcript was also detectable in the aphid body remaining after removal of the head, gut and embryo, probably due to its extensive pattern of expression in insects. For example, the NPFR transcript has been observed in the testis, midgut, ovary, brain, fat body, epidermis, Malpighian tubule, and silk gland of B. mori larvae (Deng et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

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Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Qu²,

Zhang

et al. 2018

Front. Physiol.

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Neuropeptide F (NPF) signaling systems are widespread and highly evolutionarily conserved from vertebrates to invertebrates. In fact, NPF has been identified in many insect species and plays regulatory roles in diverse physiological processes, such as feeding, learning, reproduction and stress responses. NPF operates by interacting with the NPF receptor (NPFR). Here, we characterized and determined the presumed role of NPF signaling in the wingless parthenogenetic pea aphid, Acyrthosiphon pisum. Quantitative real-time reverse transcription-PCR (qRT-PCR) revealed that the expression levels of both NPF and NPFR transcripts varied across developmental stages, which implies that the NPF signaling system might participate in the developmental regulation of aphid physiological processes or behaviors. The NPF transcript was mainly detected in the head but not in the gut, whereas the NPFR transcript was mainly detected in both the gut and head. In addition, the NPF transcript levels were markedly up-regulated in starved aphids compared with satiated aphids, and the transcript levels recovered after re-feeding. In contrast, the NPFR transcript levels remained stable in starved and re-fed aphids. Furthermore, RNAi knockdown by the injection of NPF dsRNA into wingless adult aphids significantly reduced their food intake. Further analysis of the modification of aphid feeding behavior on broad bean plants using electrical penetration graphs (EPGs) revealed that both the probing time and the total duration of phloem activity decreased significantly in the NPF treatment group. These results indicated a lower appetite for food after NPF knockdown, which could explain the reduction in aphid food intake. NPF silencing was also shown to reduce reproduction but not survival in aphids. Overall, the results of these experiments suggest that NPF plays an important role in regulation of feeding in A. pisum.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Qu²,

Zhang

et al. 2018

Front. Physiol.

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“…2 ). Neuropeptide receptor A4 has been identified as a NPF receptor 47 , whereas A10 and A11 are sNPF receptors 14 . Although A7 clusters nicely with A10 and A11, the ligand binding specificity of A7 has not been determined.…”

Section: Resultsmentioning

confidence: 99%

Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

Teng

et al. 2016

Sci Rep

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In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding neuropeptides and their associated GPCRs in the rice stem borer Chilo suppressalis were identified by a transcriptomic analysis and were used to identify potential targets for the disruption of physiological processes and the protection of crops. Forty-three candidate genes were found to encode the neuropeptide precursors for all known insect neuropeptides except for arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like precursors 2-4 (NPLP2-4), and proctolin. In addition, novel alternative splicing variants of three neuropeptide genes (allatostatin CC, CCHamide 1, and short neuropeptide F) are reported for the first time, and 51 putative neuropeptide GPCRs were identified. Phylogenetic analyses demonstrated that 44 of these GPCRs belong to the A-family (or rhodopsin-like), 5 belong to the B-family (or secretin-like), and 2 are leucine-rich repeat-containing GPCRs. These GPCRs and their likely ligands were also described. qRT-PCR analyses revealed the expression profiles of the neuropeptide precursors and GPCR genes in various tissues of C. suppressalis. Our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in Lepidoptera and aid in the design of peptidomimetics, pseudopeptides or small molecules capable of disrupting the physiological processes regulated by these signaling molecules and their receptors.

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“…Hamkalo et al showed that no notable differences was found on the photosynthetic parameters of bombyx among the inter- silkworm and monoculture at the jointing stage in bombyx/soyworm system , while the Pn, Tr, Gs , and Ci of bombyx were significant higher in the treatments of no gut separation than that gut separation treatments 30 . System citrumelo worms with perennial grass was effective in Fe fodder capture and dry matter weight which associated with GLOP index, preventing the development of leaf chlorosis and improving their growth compared to the control worms 31–33 . The results indicated that the accumulation of morphological and functional pliability derived from gut collaborations by intra-gut for enhancing nutrient acquisition in system and the importance of pliability in the performance of over-yielding advantage of system .…”

Section: Discussionmentioning

confidence: 97%

The effect of symbiosis between silkworm (Bombyx mori) andFirmicuteson silk production

Medina

2018

Preprint

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Abstract:31 Silkworm conditioning systems are widely popular due to enhancements observed in 32 productivity and in resource efficiencies. However, limited knowledge is available on 33 how intra-gut interspecific collaboration between the work and gut bacteria affects 34 silk dry matter biomass production. The study was to study how gut bacteria, 35specifically fermicutes boost the dry silk production in Bombyx mori by 36 altruistic/symbiotic interactions. 37 Materials and methods 38Greenhouse experiments were carried out to test the yield, biomass, nutrient uptake, Results 44The yield of silk production was increased by 53.6% and 27.8% in the treatments with 51The worm length, leave number and net photosynthetic rate of silkworm were 52 significantly boosted, while there is no significant effect on firmicutes. 53 Conclusions 54The improvement of yield and nutrient acquisition may result from silkworm (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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Activation of Bombyx neuropeptide G protein-coupled receptor A4 via a Gαi-dependent signaling pathway by direct interaction with neuropeptide F from silkworm, Bombyx mori

Cited by 25 publications

References 33 publications

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

The effect of symbiosis between silkworm (Bombyx mori) andFirmicuteson silk production

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