Activation of an alfalfa cyclin‐dependent kinase inhibitor by calmodulin‐like domain protein kinase

Pettkó‐Szandtner, Aladár; Mészáros, Tamás; Horváth, Gábor; Bako, L.; Csordás-Tóth, Éva; Blastyák, András; Zhiponova, Miroslava; Miskolczi, Pál; Dudits, Dénes

doi:10.1111/j.1365-313x.2006.02677.x

Cited by 51 publications

(52 citation statements)

References 59 publications

(164 reference statements)

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“…Previous work indicated a possible role for KRPs in stress response regulation. In Arabidopsis, KRP1 is known to respond to ABA (Wang et al, 1998), and an alfalfa KRP was induced both after mild salt treatment and ABA application (Pettko-Szandtner et al, 2006). Finally, we show a possible involvement of transcriptional regulation of the E2F/DP/RBR pathway in mediating responses to environmental conditions.…”

Section: Transcriptional Analysis Of Cell Cycle Genes Under Environmementioning

confidence: 78%

“…Mild salt stress leads to loss of CDK activity and to reduced promoter activity of CYCB1;2 (West et al, 2004). Finally, the production of a CDK activity inhibitor, KIP-related protein (KRP), is stimulated by abscisic acid (ABA) in Arabidopsis (KRP1) and alfalfa (Medicago sativa; KRPMt), suggesting a role for this gene in the ABA-mediated stress response pathway (Wang et al, 1998;Pettko-Szandtner et al, 2006).…”

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confidence: 99%

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Cold Nights Impair Leaf Growth and Cell Cycle Progression in Maize through Transcriptional Changes of Cell Cycle Genes

Rymen

Fiorani

Kartal³

et al. 2007

Plant Physiology

206

162

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Low temperature inhibits the growth of maize (Zea mays) seedlings and limits yield under field conditions. To study the mechanism of cold-induced growth retardation, we exposed maize B73 seedlings to low night temperature (25°C /4°C, day/ night) from germination until the completion of leaf 4 expansion. This treatment resulted in a 20% reduction in final leaf size compared to control conditions (25°C/18°C, day/night). A kinematic analysis of leaf growth rates in control and cold-treated leaves during daytime showed that cold nights affected both cell cycle time (165%) and cell production (222%). In contrast, the size of mature epidermal cells was unaffected. To analyze the effect on cell cycle progression at the molecular level, we identified through a bioinformatics approach a set of 43 cell cycle genes and analyzed their expression in proliferating, expanding, and mature cells of leaves exposed to either control or cold nights. This analysis showed that: (1) the majority of cell cycle genes had a consistent proliferation-specific expression pattern; and (2) the increased cell cycle time in the basal meristem of leaves exposed to cold nights was associated with differential expression of cell cycle inhibitors and with the concomitant down-regulation of positive regulators of cell division.

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Section: Transcriptional Analysis Of Cell Cycle Genes Under Environmementioning

confidence: 78%

mentioning

confidence: 99%

Cold Nights Impair Leaf Growth and Cell Cycle Progression in Maize through Transcriptional Changes of Cell Cycle Genes

Rymen

Fiorani

Kartal³

et al. 2007

Plant Physiology

206

162

View full text Add to dashboard Cite

show abstract

“…Based on our hypothesis that CDKA;1 is assisted by B-type CDKs during mitosis, one would expect weak ICK/KRP misexpression plants to have reduced rather than increased levels of endoreplication. However, the preference of ICK/KRPs to A-type CDKs is restricted to yeast two-hybrid data, and strikingly, a recent report using purified CDK complexes and adding ICK/KRP to them found that a B-type CDK complex was most susceptible to CKIs (Pettko-Szandtner et al, 2006). Thus, it will be an important task in the future to decipher the full spectrum of ICK/KRP targets.…”

Section: Low Cdk Activity and Plant Developmentmentioning

confidence: 99%

T-Loop Phosphorylation ofArabidopsisCDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate Residue

et al. 2007

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As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases. Phosphorylation of a canonical Thr residue in the T-loop of the kinases is required for high enzyme activity in animals and yeast. We show that the Arabidopsis thaliana Cdc2 1 /Cdc28 homolog CDKA;1 is also phosphorylated in the T-loop and that phosphorylation at the conserved Thr-161 residue is essential for its function. A phospho-mimicry T161D substitution restored the primary defect of cdka;1 mutants, and although the T161D substitution displayed a dramatically reduced kinase activity with a compromised ability to bind substrates, homozygous mutant plants were recovered. The rescue by the T161D substitution, however, was not complete, and the resulting plants displayed various developmental abnormalities. For instance, even though flowers were formed, these plants were completely sterile as a result of a failure of the meiotic program, indicating that different requirements for CDKA;1 function are needed during plant development.

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“…Earlier reports also showed that various stresses can induce cell cycle inhibitors of the CDK inhibitor (ICK)/KRP or SIM family and thereby arrest the cell cycle (Wang et al, 1998;Pettkó-Szandtner et al, 2006;De Veylder et al, 2007;Peres et al, 2007). Furthermore, DELLAs appear to be able to control cell proliferation rates by regulating KRP2, SIM, SIM-RELATED1 (SMR1), and SMR2 transcription (Achard et al, 2009).…”

mentioning

confidence: 99%

DELLA Signaling Mediates Stress-Induced Cell Differentiation in Arabidopsis Leaves through Modulation of Anaphase-Promoting Complex/Cyclosome Activity

et al. 2012

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Drought is responsible for considerable yield losses in agriculture due to its detrimental effects on growth. Drought responses have been extensively studied, but mostly on the level of complete plants or mature tissues. However, stress responses were shown to be highly tissue and developmental stage specific, and dividing tissues have developed unique mechanisms to respond to stress. Previously, we studied the effects of osmotic stress on dividing leaf cells in Arabidopsis (Arabidopsis thaliana) and found that stress causes early mitotic exit, in which cells end their mitotic division and start endoreduplication earlier. In this study, we analyzed this phenomenon in more detail. Osmotic stress induces changes in gibberellin metabolism, resulting in the stabilization of DELLAs, which are responsible for mitotic exit and earlier onset of endoreduplication. Consequently, this response is absent in mutants with altered gibberellin levels or DELLA activity. Mitotic exit and onset of endoreduplication do not correlate with an up-regulation of known cell cycle inhibitors but are the result of reduced levels of DP-E2F-LIKE1/E2Fe and UV-B-INSENSITIVE4, both inhibitors of the developmental transition from mitosis to endoreduplication by modulating anaphase-promoting complex/cyclosome activity, which are down-regulated rapidly after DELLA stabilization. This work fits into an emerging view of DELLAs as regulators of cell division by regulating the transition to endoreduplication and differentiation.

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Activation of an alfalfa cyclin‐dependent kinase inhibitor by calmodulin‐like domain protein kinase

Cited by 51 publications

References 59 publications

Cold Nights Impair Leaf Growth and Cell Cycle Progression in Maize through Transcriptional Changes of Cell Cycle Genes

Cold Nights Impair Leaf Growth and Cell Cycle Progression in Maize through Transcriptional Changes of Cell Cycle Genes

T-Loop Phosphorylation ofArabidopsisCDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate Residue

DELLA Signaling Mediates Stress-Induced Cell Differentiation in Arabidopsis Leaves through Modulation of Anaphase-Promoting Complex/Cyclosome Activity

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