Action of Various Anticoagulants on Hemolymphs of Lobsters and Spiny Lobsters

Abstract

Effects of anticoagulants on hemolymph coagulation of lobsters are reported. Effects of some inhibitors on plasma clotting change in relation to molting stages of animals. Variations of electrophoretic and immunochemical protein patterns are analyzed. Complete coagulation inhibition shows that the plasma coagulogen exists as a mixture of soluble uncovalently crosslinked polymers. In lobsters the molecular weight of the lightest visible circulating unit is approximately 420,000 daltons. The weight interval between each of the four lighter polymers is about 200,000 daltons. Serine protease inhibitors impair plasma clotting and interact with cellular clumping.

Characterisation of a natural haemagglutinin with affinity for acetylated aminosugars in the serum of the marine prawn, (H. Milne Edwards)

“…DIVALENT CATION DEPENDENCY AND EDTA/EGTA SENSITIVITY The initial haemagglutinating activity of serum samples (untreated) was determined in TBS containing 10 mM CaCl 2 (TBS-III) since Ca 2+ is known to be depleted during haemolymph coagulation in crustaceans (Durliat & Vranckx, 1981). Subsequently, serum samples (each 500 l) were dialysed (MW exclusion limit <10 000) extensively against TBS-I (to test divalent cation dependency) or in TBS-V (to examine EDTA sensitivity) at 15 C. The samples dialysed against TBS-V were subsequently re-equilibrated by dialysis in TBS-I.…”

Barnacle cement: a polymerization model based on evolutionary concepts

“…Integrity of assembled fibrin monomers is brought about through transglutaminase-mediated cross-linking (Lorand et al, 1962;Shen and Lorand, 1983;Lorand, 2000;Collet et al, 2005). Invertebrate blood coagulation involves similar enzymatic mechanisms: converging cascades of trypsin-like serine proteases in horseshoe crabs (Muta and Iwanaga, 1996;Iwanaga et al, 1998;Iwanaga, 2002); a transglutaminase (Fuller and Doolittle, 1971;Kopacek et al, 1993;Chen et al, 2005) showing homology to vertebrate transglutaminase (Wang et al, 2001); and proteolytic activation (Durliat and Vranckx, 1981;Madaras et al, 1981;Theopold et al, 2004) in crustaceans.…”

Tissue-specific expression and regulation of the haemolymph clottable protein of tiger shrimp (Penaeus monodon)

“…The plasma CPs from various crustacean species have been characterized [4e9, 12,13]. Lobster CP is a homodimeric glycoprotein of about 400 kDa which forms stable clots in the wound area [12].…”

“…Lobster CP is a homodimeric glycoprotein of about 400 kDa which forms stable clots in the wound area [12]. Its crystal structure was elucidated by single particle reconstitution and a possible site for lipid-binding was identified [14].…”