Actin related protein complex subunit 1b controls sperm release, barrier integrity and cell division during adult rat spermatogenesis

Abstract

Actin remodeling is a vital process for signaling, movement and survival in all cells. In the testes, extensive actin reorganization occurs at spermatid-Sertoli cell junctions during sperm release (spermiation) and at inter Sertoli cell junctions during restructuring of the blood testis barrier (BTB). During spermiation, tubulobulbar complexes (TBCs), rich in branched actin networks, ensure recycling of spermatid-Sertoli cell junctional molecules. Similar recycling occurs during BTB restructuring around the same time as spermiation occurs. Actin related protein 2/3 complex is an essential actin nucleation and branching protein. One of its subunits, Arpc1b, was earlier found to be down-regulated in an estrogen-induced rat model of spermiation failure. Also, Arpc1b was found to be estrogen responsive through estrogen receptor beta in seminiferous tubule culture. Here, knockdown of Arpc1b by siRNA in adult rat testis led to defects in spermiation caused by failure in TBC formation. Knockdown also compromised BTB integrity and caused polarity defects of mature spermatids. Apart from these effects pertaining to Sertoli cells, Arpc1b reduction perturbed ability of germ cells to enter G2/M phase thus hindering cell division. In summary, Arpc1b, an estrogen responsive gene, is a regulator of spermiation, mature spermatid polarity, BTB integrity and cell division during adult spermatogenesis.

Transcriptome analysis reveals the genes involved in spermatogenesis in white feather broilers

“…Studies have demonstrated that inhibition of the CD74 signaling pathway induced testicular immunosuppression and fibrosis ( Li et al, 2022 ). ARPC1B is a regulator of spermatogenesis, the blood-testis barrier and cell division ( Kumar et al, 2016 ). APOA1 mRNA exists in sperm and plays an important role in maintaining sperm motility, survival rate and fertilization process ( Chi et al, 2022 ).…”

Integrated Comparative Transcriptome and circRNA-lncRNA-miRNA-mRNA ceRNA Regulatory Network Analyses Identify Molecular Mechanisms Associated with Intramuscular Fat Content in Beef Cattle

“…As the protein coded by this gene is present in the APR2/3 complex, it may have a role in the organization of the actin cytoskeleton and function as an ATP-binding component of the Arp2/3 complex, which is involved in the regulation of actin polymerization. This complex is a regulatory protein in the actin cytoskeleton and induces cell-shape change and motility [ 64 , 65 ]. Actin filament-based process, actin cytoskeleton organization, actin binding, cytoskeletal protein binding and actin cytoskeleton were the highly associated GO terms affected by the ACTR3B gene.…”

Transcriptomic analysis of mechanism underlying the effect of induced molting on semen quality and reproductive performance in aged Houdan roosters

“…To date, 4 cytoskeletons (actin, micro, intermediate filament, and septum) supporting spermatogenesis are found in the testis. Regulation of actin cytoskeleton as one of them plays a crucial role in mammalian testicular spermatogenesis ( Kumar et al, 2016 ; Tang et al, 2016 ; Liu et al, 2020 ). In this study, through KEGG enrichment analysis, we found a significantly enriched pathway regulation of actin cytoskeleton, and differential genes in the regulation of actin cytoskeleton pathway were validated by qRT-PCR.…”