Acrolein-Induced Oxidative Stress and Cell Death Exhibiting Features of Apoptosis in the Yeast Saccharomyces cerevisiae Deficient in SOD1

Kwolek‐Mirek, Magdalena; Zadrąg‐Tęcza, Renata; Bednarska, Sabina; Bartosz, Grzegorz

doi:10.1007/s12013-014-0376-8

Cited by 25 publications

(11 citation statements)

References 59 publications

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“…A strain that was deleted for gene Afu1g09190 (AFUB_008610) was highly sensitive to AA ( Fig 1A and 1B ) but presented no growth defects in non-stress conditions ( S1A and S1B Fig ) nor in the presence of 2-deoxyglucose (2DG), an additional indicator for defects in CCR[ 15 ] ( S1C Fig ). AA is converted by alcohol dehydrogenase (ADH) to acrolein, a highly toxic compound shown to also induce oxidative stress within the cell[ 16 , 17 ]. To investigate any ADH regulatory or activity defects, enzyme activity was measured in the presence of ethanol and ethanol and glucose, with glucose causing the CCR-mediated transcriptional repression of ADH-encoding genes[ 18 ].…”

Section: Resultsmentioning

confidence: 99%

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

Ries

Pardeshi

Dong³

et al. 2020

PLoS Pathog

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Aspergillus fumigatus is an opportunistic fungal pathogen that secretes an array of immune-modulatory molecules, including secondary metabolites (SMs), which contribute to enhancing fungal fitness and growth within the mammalian host. Gliotoxin (GT) is a SM that interferes with the function and recruitment of innate immune cells, which are essential for eliminating A . fumigatus during invasive infections. We identified a C6 Zn cluster-type transcription factor (TF), subsequently named RglT, important for A . fumigatus oxidative stress resistance, GT biosynthesis and self-protection. RglT regulates the expression of several gli genes of the GT biosynthetic gene cluster, including the oxidoreductase-encoding gene gliT , by directly binding to their respective promoter regions. Subsequently, RglT was shown to be important for virulence in a chemotherapeutic murine model of invasive pulmonary aspergillosis (IPA). Homologues of RglT and GliT are present in eurotiomycete and sordariomycete fungi, including the non-GT-producing fungus A . nidulans , where a conservation of function was described. Phylogenetically informed model testing led to an evolutionary scenario in which the GliT-based resistance mechanism is ancestral and RglT-mediated regulation of GliT occurred subsequently. In conclusion, this work describes the function of a previously uncharacterised TF in oxidative stress resistance, GT biosynthesis and self-protection in both GT-producing and non-producing Aspergillus species.

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Section: Resultsmentioning

confidence: 99%

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

Ries

Pardeshi

Dong³

et al. 2020

PLoS Pathog

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“…It is sensitive to a variety of stress agents due to escalated oxidative stress. Moreover, it also plays a key role in the apoptotic process [ 21 ]. Yca1 mediates the apoptotic process, and encodes a yeast protein with structural homology and similar function to mammalian caspases [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility assessment of single-walled carbon nanotubes using Saccharomyces cerevisiae as a model organism

Zhu

Luo

et al. 2018

J Nanobiotechnol

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BackgroundSingle-walled carbon nanotubes (SWCNTs) have many potential applications in various fields. Especially, the unique physicochemical properties make them as the prime candidates for applications in biomedical fields. However, biocompatibility of SWCNTs has been a major concern for their applications. In the study, biocompatibility of oxidized SWCNTs (O-SWCNTs) was assessed using Saccharomyces cerevisiae (S. cerevisiae) as a model organism.ResultsCell proliferation and viability were significantly changed after exposure to O-SWCNTs (188.2 and 376.4 mg/L) for 24 h. O-SWCNTs were internalized in cells and distributed in cytoplasm, vesicles, lysosomes and cell nucleus. The average O-SWCNTs contents in S. cerevisiae were ranged from 0.18 to 4.82 mg/g during the exposure from 0 to 24 h, and the maximum content was reached at 18 h after exposure. Both penetration and endocytosis were involved in the internalization of O-SWCNTs in S. cerevisiae, and endocytosis was the main pathway. Cellular structures and morphology were changed after exposure to O-SWCNTs, such as undulating appearance at the membrane, shrinking of the cytosol, increased numbers of lipid droplets and disruption of vacuoles. ROS and antioxidant enzymes activities were observably changed following exposure. For the treatment at 376.4 mg/L, 20.8% of the total cells was undergone apoptosis. Decrease of mitochondrial transmembrane potential and leakage of cytochrome c from mitochondria were observed after exposure. Moreover, expression levels of apoptosis-related genes were significantly increased.ConclusionsO-SWCNTs can internalize in S. cerevisiae cells via direct penetration and endocytosis, and distribute in cytoplasm, vesicles, lysosomes and cell nucleus. Besides, O-SWCNTs (188.2 and 376.4 mg/L) can induce apoptosis in S. cerevisiae cells, and oxidative stress is involved in activation of the mitochondria-dependent apoptotic pathway.

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“…It has been reported that acrolein, one of the metabolites of CP, is one of the main responsible for the increased production of reactive oxygen species (RS) and lipid peroxidation, which is one of the possible mechanisms responsible for its toxicity [ 5 ]. Oxidative stress is a key factor in the etiology of male infertility, since increased testicular oxidative stress can cause changes in the dynamics of testicular microvascular blood flow and endocrine signaling leading to an increase in germ cell apoptosis and subsequent hypospermatogenesis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Tribulus terrestris Protects against Male Reproductive Damage Induced by Cyclophosphamide in Mice

Pavin

Izaguirry

Soares

et al. 2018

Oxidative Medicine and Cellular Longevity

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Tribulus terrestris (TT) has been considered as a potential stimulator of testosterone production, which has been related with steroidal saponins prevailing in this plant. Cyclophosphamide (CP) is the most commonly used anticancer and immunosuppressant drug, which causes several toxic effects, especially on the reproductive system. Patients who need to use CP therapy exhibit reduced fertility or infertility, which impacts both physically and emotionally on the decision to use this drug, especially among young men. We hypothesized that the treatment with TT dry extract would protect the male reproductive system against CP toxicity. Mice received dry extract of TT (11 mg/kg) or vehicle by gavage for 14 days. Saline or CP was injected intraperitoneally at a single dose (100 mg/kg) on the 14th day. Animals were euthanized 24 h after CP administration, and testes and epididymis were removed for biochemical and histopathological analysis and sperm evaluation. The dry extract of TT was evaluated by HPLC analysis and demonstrated the presence of protodioscin (1.48%, w/w). CP exposure increased lipid peroxidation, reactive species, and protein carbonylation and altered antioxidant enzymes (SOD, CAT, GPx, GST, and GR). Moreover, acute exposure to CP caused a reduction on 17 β-HSD activity, which may be related to the reduction in serum testosterone levels, histopathological changes observed in the testes, and the quality of the semen. The present study highlighted the role of TT dry extract to ameliorate the alterations induced by CP administration in mice testes, probably due to the presence of protodioscin.

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Acrolein-Induced Oxidative Stress and Cell Death Exhibiting Features of Apoptosis in the Yeast Saccharomyces cerevisiae Deficient in SOD1

Cited by 25 publications

References 59 publications

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence

Biocompatibility assessment of single-walled carbon nanotubes using Saccharomyces cerevisiae as a model organism

Tribulus terrestris Protects against Male Reproductive Damage Induced by Cyclophosphamide in Mice

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