Acquired pellicle protein-based engineering protects against erosive demineralization

Carvalho, Thamyris de Souza; Araújo, Tamara Teodoro; Ventura, Talita Mendes Oliveira; Dionízio, Aline; Câmara, João Víctor Frazão; Moraes, Samanta Mascarenhas; Pelá, Vinícius Taioqui; Martini, Tatiana; Leme, Julia Chaparro; Derbotolli, Ana Luiza Bogaz; Grizzo, Larissa Tercília; Crusca, Edson; Shibao, Priscila Yumi Tanaka; Marchetto, Reinaldo; Henrique-Silva, Flávio; Pessan, Juliano Pelim; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.jdent.2020.103478

Cited by 41 publications

(70 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is in-creased in the AEP [Martini et al, 2019] and saliva [Martini et al, 2020] of gastroesophageal reflux disease patients without erosive tooth wear compared to gastroesophageal reflux disease patients presenting erosive tooth wear. The presence of hemoglobin in the longterm AEP after rinsing with StatpSpS is consistent with our recent study showing a 3-fold increase in hemoglobin in the AEP formed 2 h after rinsing with a solution containing StatpSpS and challenged with 1% citric acid (pH 2.5) for 10 s [Carvalho et al, 2020]. Anyway, it is intriguing why hemoglobin was found exclusively in the control group in the short-term and solely in the treated group in the long-term.…”

Section: Discussionsupporting

confidence: 91%

“…Our group recently reported that rinsing with Statp-SpS increases acid-resistant proteins in the AEP, which in turn, reduces enamel demineralization in vivo [Carvalho et al, 2020]. In that study, the volunteers rinsed with 10 mL of phosphate buffer containing 1.88 × 10 −5 M Statp-SpS and the pellicle was allowed to form for 2 h (as done in the present study).…”

Section: Discussionmentioning

confidence: 70%

“…Some proteins, such as cystatin-9 and cystatin-C, were identified only when the volunteers rinsed with the StatpSpS solution. Moreover, the treatment with StatpSpS significantly reduced Ca released from enamel in comparison with the control solution [Carvalho et al, 2020]. These results show that StatpSpS preferably binds to enamel during the 1-min rinse, altering the composition of the AEP that is subsequently formed.…”

Section: Introductionmentioning

confidence: 66%

“…Moreover, cystatin-9 and cystatin-C were only identified upon treatment with Statp-SpS. The increase in acid-resistant proteins in the study by Carvalho et al [2020] indicated that, upon rinse with StatpSpS, due to the high affinity of the peptide by hydroxyapatite, it binds to the enamel surface, altering the basal layer of the AEP. The alteration in the basal layer will change the subsequent layers of the AEP, since the proteins that constitute the subsequent layers interact with the precursor proteins [Siqueira et al, 2012].…”

Section: Discussionmentioning

confidence: 91%

“…This peptide, at the concentration of 1.88 × 10 −5 M, was shown to protect against initial intrinsic erosion in vitro [Taira et al, 2020]. Moreover, a recent in vivo proof of concept study was conducted to evaluate the proteomic alterations in the AEP after rinsing for 1 min with 1.88 × 10 −5 M StatpSpS before the formation of the AEP and subsequent erosive challenge (1% citric acid, pH 2.5, 10 s), as well as the protection of this treatment against erosive demineralization [Carvalho et al, 2020]. Rinsing with StatpSpS significantly increased several acid-resistant proteins in the AEP.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle

et al. 2021

Self Cite

View full text Add to dashboard Cite

Changes in the proteomic profile of the acquired enamel pellicle (AEP) formed for 3 min or 2 h after rinsing with a peptide containing the 15 N-terminal residues of statherin, with serines 2 and 3 phosphorylated (StatpSpS), were evaluated. Nine volunteers participated in 2 consecutive days. Each day, after professional tooth cleaning, they rinsed for 1 min with 10 mL of phosphate buffer containing 1.88 × 10<sup>−5</sup> M StatpSpS or phosphate buffer only (control). The acquired pellicle formed on enamel after 3 min or 2 h was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. In the 3-min AEP, 19 and 131 proteins were uniquely identified in the StatpSpS and control groups, respectively. Proteins typically found in the AEP were only found in the latter. Only 2 proteins (neutrophil defensins) were increased upon treatment with StatpSpS, while 65 proteins (among which are several typical AEP proteins) were decreased. In the 2-h AEP, 50 and 108 proteins were uniquely found in StatpSpS and control groups, respectively. Hemoglobin subunits and isoforms of keratin were only found in the StatpSpS group, while cystatin-C, cathepsin D, and cathepsin G, isoforms of heat shock 70 and protocadherin were exclusively found in the control group. In addition, 23 proteins were increased upon treatment with StatpSpS, among which are histatin-1, serum albumin, and isoforms of neutrophil defensin and keratin, while 77 were decreased, most of them were typical AEP proteins. In both evaluated periods, rinsing with StatpSpS profoundly changed the proteomic profile of the AEP, which might impact the protective role of this integument against carious or erosive demineralization. This study provides important insights on the dynamics of the protein composition of the AEP along time, after rinsing with a solution containing StatpSpS.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations