Acquired antigenicity of DNA after modification with peroxynitrite

Habib, Safia; Moinuddin, Mohammed; Ali, Rashid

doi:10.1016/j.ijbiomac.2005.02.005

Cited by 40 publications

(24 citation statements)

References 18 publications

(9 reference statements)

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“…2). The damaged DNA has been reported to be highly immunogenic, implicating its role in the production of antibodies in diseases like SLE and Cancer [42][43][44].…”

Section: Indirect Effect Of Nomentioning

confidence: 99%

Biochemistry of Nitric Oxide

2011

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Nitric oxide (NO) a free radical having both cytoprotective as well as tumor promoting agent is formed from L-arginine by converting it to L-citrulline via nitric oxide synthase enzymes. The reaction product of nitric oxide with superoxide generates potent oxidizing agent, peroxynitrite which is the main mediator of tissue and cellular injury. Peroxynitrite is reactive towards many biomolecules which includes amino acids, nucleic acid bases; metal containing compounds, etc. NO metabolites may play a key role in mediating many of the genotoxic/ carcinogenic effects as DNA damage, protein or lipid modification, etc. The basic reactions of nitric oxide can be divided as direct effect of the radical where it alone plays a role in either damaging or protecting the cell milieu and an indirect effect in which the byproducts of nitric oxide formed by convergence of two independent radical generating pathways play the role in biological reactions which mainly involve oxidative and nitrosative stress. Nitric oxide is also capable of directly interacting with mitochondria through inhibition of respiration or by permeability transition. Reaction of nitric oxide with metal ions include its direct interaction with the metals or with oxo complexes thereby reducing them to lower valent state. Excessive production of nitric oxide can be studied by inhibiting the synthetic pathway of nitric oxide using both selective or specific nitric oxide synthase inhibitor or nonselective nitric oxide synthase inhibitor with respect to isoforms of nitric oxide.Keywords Nitric oxide Á Nitric oxide synthase Á Nitric oxide inhibitors Á Generation of nitric oxide Á Cancer Á Systemic lupus erythematosus Á Direct and indirect effect of nitric oxide Á Effect of nitric oxide on mitochondria

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“…2). The damaged DNA has been reported to be highly immunogenic, implicating its role in the production of antibodies in diseases like SLE and Cancer [42][43][44].…”

Section: Indirect Effect Of Nomentioning

confidence: 99%

Biochemistry of Nitric Oxide

2011

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“…The antigen-binding specificity of the antibodies was evaluated by competition ELISA [16]. Varying amounts of inhibitor (0 -20 g/ml) were mixed with a constant amount of antiserum or IgG.…”

Section: Competition Elisamentioning

confidence: 99%

Preferential recognition of Amadori-rich lysine residues by serum antibodies in diabetes mellitus: Role of protein glycation in the disease process

et al. 2009

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“…Besides, NO and its products have been reported to modify the structure of histones, collagen, and the structure and function of lipid molecules; effects on other macromolecules include altering the structure of DNA and rendering it immunogenic [25,26]. The potential role of nitrosative modifications affects the overall regulation of cellular functions (Figure 27.2).…”

Section: Role Of Nitric Oxide In Nutritional Supplementsmentioning

confidence: 99%