Acquired antibiotic resistance in lactic acid bacteria from food

Teuber, Michael; Meile, Léo; Schwarz, Franziska

doi:10.1007/978-94-017-2027-4_5

Cited by 153 publications

(232 citation statements)

References 53 publications

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“…Using a 120 μg gentamicin disc, as a reliable indicator of high level gentamicin (HLG) resistance [60], we also found out that 12 (21.05%) enterococcal isolates showed HLG resistance. Similar results were obtained by other authors [61][62][63]. Prevalence of vancomycin resistance among enterococcal isolates was 29.82%.…”

Section: Discussionsupporting

confidence: 91%

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

et al. 2015

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Sjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheep's milk in the southern part of Serbia -Sjenica Pester plateau.The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated.During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 10 5 cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identifi ed as Staphylococcus intermedius (11 isolates) and Staphylococcus aureus (1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profi le in coagulase positive staphylococci.Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profi le in enterococci.All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins, tet(M) and tet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showed tet(M) gene presence, while 2 lactobacilli isolates revealed the presence of tet(K) gene.

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Section: Discussionsupporting

confidence: 91%

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

et al. 2015

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“…A resistência à vancomicina em 100% das amostras de Lactobacillus e Weissella testadas foi também observada em outros trabalhos (Coppola et al, 2005;Ouoba et al, 2008;Rodríguez-Alonso et al, 2009), o que confirma que Lactobacillus e outros gêneros de bactérias ácido-láticas podem ter resistência intrínseca à vancomicina (Teuber et al, 1999 Em concordância com os resultados encontrados neste trabalho, diversos autores encontraram altas porcentagens de resistência à oxacilina em estudos que avaliaram a susceptibilidade a este antimicrobiano (Coppola et al, 2005;Rodríguez-Alonso et al, 2009). Mesmo pertencendo ao grupo dos β-lactâmicos, geralmente as taxas de resistência à oxacilina são maiores do que para penicilina (Teuber et al, 1999).…”

Section: Resultsunclassified

“…Mesmo pertencendo ao grupo dos β-lactâmicos, geralmente as taxas de resistência à oxacilina são maiores do que para penicilina (Teuber et al, 1999). Neste trabalho, 33% das amostras de bactérias ácido-lácticas foram resistentes à penicilina e 100% à oxacilina.…”

Section: Resultsunclassified

Potencial probiótico in vitro de bactérias ácido-láticas isoladas de queijo-de-minas artesanal da Serra da Canastra, MG

Costa

Souza

Acúrcio

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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RESUMOO potencial probiótico in vitro de 12 amostras de bactérias ácido-láticas (11 Lactobacillus spp. e uma Weissella paramesenteroides), isoladas de queijo-de-minas artesanal da Serra da Canastra, foi investigado. Essas amostras foram caracterizadas in vitro quanto à susceptibilidade a antimicrobianos, antagonismo contra microrganismos de referência patogênicos e não patogênicos e sensibilidade a pH gástrico e sais biliares. (Gilliland, 1985).

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“…At present, there is great concern that animal and human commensal bacteria, such as LAB, could act as a reservoir for antibiotic resistance genes. These microorganisms may subsequently contaminate the raw milk and meat produced from these animals, and the foods prepared from those raw materials can therefore be considered as potential vehicles for the spread of antibiotic-resistant LAB along the food chain to the consumer (42). Resistances could ultimately be transferred to human pathogenic and opportunistic bacteria, hampering the treatment of infections (27).…”

Section: Discussionmentioning

confidence: 99%

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmidcarried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 g/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 g/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium.

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Acquired antibiotic resistance in lactic acid bacteria from food

Cited by 153 publications

References 53 publications

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

Potencial probiótico in vitro de bactérias ácido-láticas isoladas de queijo-de-minas artesanal da Serra da Canastra, MG

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

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