Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., to accommodate Acinetobacter genomic species 10 and 11, respectively

Abstract

Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA–DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA–DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize d-glucose and to grow at 38 °C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003T (=CIP 70.12T =ATCC 17924T) and that of A. guillouiae sp. nov. is LMG 988T (=CIP 63.46T =ATCC 11171T =CCUG 2491T).

Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant

“…Although many species of Acinetobacter are reported as PGPR (Indiragandhi et al, 2008;Gulati et al, 2009;Kang et al, 2009;Shi et al, 2011), this SW5 strain showed a high plant growth promoting capability. SW5 strain has a 97.875% of sequence homology of 16S rRNA gene compared with the type strain A. guillouiae ATCC 11171 T reported recently by Nemec et al (2010), and some general characteristics of SW5 strain were coincided with those of other Acinetobacter spp. (Baumann et al, 1968).…”

Biochemical and Genetic Characterization of Carbapenem-Hydrolyzing β-Lactamase OXA-229 from Acinetobacter bereziniae

“…Reference strain Escherichia coli TOP10 was used as a host for clonings. A. bereziniae strain CIP70.12, isolated from a human wound in 1970, was used as the reference strain (15).…”

“…A. bereziniae is a Gram-negative, strictly aerobic, oxidase-negative, and nonmotile coccobacillus (15). A. bereziniae was found to be responsible for health care-associated infections, including severe sepsis in an immunocompromised patient (12).…”

Bacteremia Due to Acinetobacter Genomic Species 10

“…nov. (13), have previously been identified in clinical samples, they were subjected to studies of identification methods without mention of clinical relevance (10,15). In this study, we identified AGS 10 isolates from six blood samples.…”