Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures

Shashkov, Alexander S.; Cahill, Sarah; Arbatsky, Nikolay P.; Westacott, Amy C.; Kasimova, Anastasiya A.; Shneider, Mikhail M.; Popova, Anastasiya V.; Shagin, Dmitry A.; Shelenkov, Andrey; Mikhaǐlova, Yu. V.; Yanushevich, Yurii G.; Edelstein, Mikhail V.; Kenyon, Johanna J.; Knirel, Yuriy A.

doi:10.1016/j.carres.2019.107774

Cited by 30 publications

(30 citation statements)

References 17 publications

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“…CPSs were isolated by phenol-water extraction from A. baumannii strains ACICU ( 18 ), LUH5549 ( 19 ), NIPH146 ( 20 ), NIPH70 ( 21 ), NIPH615 ( 22 ), LUH5547, LUH55552, B11911 ( 23 ), and MAR303 ( 24 ), belonging to K2, K32, K37, K44, K48, K87, K89, K93, and K116 capsular types, respectively. The structures of the K2, K32, K37, K44, K48, K93, and K116 CPSs were established earlier ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The spectra of depolymerasing activity of recombinant proteins were tested against a panel of A. baumannii strains with the confirmed CPS structures (Table 1) CPSs were isolated by phenol-water extraction from A. baumannii strains ACICU [17], LUH5549 [32], NIPH146 [18], NIPH70 [30], NIPH615 [31], LUH5547, LUH55552, B11911 [44] and MAR303 [45], belonging to K2, K32, K37, K44, K48, K87, K89, K93 and K116 capsular Structures of the K87 and K89 CPSs of A. baumannii LUH5547 and LUH5552, respectively, have not been reported earlier, but, according to our unpublished data, are as shown in Figure 6.…”

Section: Phage Depolymerasesmentioning

confidence: 99%

See 1 more Smart Citation

Specific Interaction of NovelFriunavirusPhages Encoding Tailspike Depolymerases with Corresponding Acinetobacter baumannii Capsular Types

Popova

Shneider

Arbatsky

et al. 2021

J Virol

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Acinetobacter baumannii is one of the most clinically important nosocomial pathogens. The World Health Organisation refers it to its «critical priority» category to develop new strategies for effective therapy. This microorganism is capable of producing structurally diverse capsular polysaccharides (CPSs), which serve as primary receptors for A. baumannii bacteriophages carrying polysaccharide-depolymerasing enzymes. In this study, eight novel bacterial viruses that specifically infect A. baumannii strains belonging to K2/K93, K32, K37, K44, K48, K87, K89 and K116 capsular types were isolated and characterized. The overall genomic architecture demonstrated that these viruses are representatives of the Friunavirus genus of the family Autographiviridae. The linear double-stranded DNA phage genomes of 41,105–42,402 bp share high nucleotide sequence identity, except for genes encoding structural depolymerases or tailspikes which determine the host specificity. Deletion mutants lacking N-terminal domains of tailspike proteins were cloned, expressed and purified. The structurally defined CPSs of the phage bacterial hosts were cleaved with the specific recombinant depolymerases, and the resultant oligosaccharides that corresponded to monomers or/and dimers of the CPS repeats (K-units) were isolated. Structures of the derived oligosaccharides were established by nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometry. The data obtained showed that all depolymerases studied were glycosidases that cleave specifically the A. baumannii CPSs by the hydrolytic mechanism, in most cases, by the linkage between the K-units. IMPORTANCE Acinetobacter baumannii, a nonfermentative, Gram-negative, aerobic bacterium, is one of the most significant nosocomial pathogens. The pathogenicity of A. baumannii is based on the cooperative action of many factors, one of them being the production of capsular polysaccharides (CPSs) that surround bacterial cells with a thick protective layer. Polymorphism of the chromosomal capsule loci is responsible for the observed high structural diversity of the CPSs. In this study, we describe eight novel lytic phages which have different tailspike depolymerases (TSDs) determining the interaction of the viruses with corresponding A. baumannii capsular types (K-types). Moreover, we elucidate the structures of oligosaccharide products obtained by cleavage of the CPSs by the recombinant depolymerases. We believe that as the TSDs determine phage specificity, the diversity of their structures should be taken into consideration as selection criteria for inclusion of certain phage candidate to the cocktail designed to control A. baumannii with different K-types.

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Section: Resultsmentioning

confidence: 99%

Section: Phage Depolymerasesmentioning

confidence: 99%

Specific Interaction of NovelFriunavirusPhages Encoding Tailspike Depolymerases with Corresponding Acinetobacter baumannii Capsular Types

Popova

Shneider

Arbatsky

et al. 2021

J Virol

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“…The most prevalent KL-types were KL9 and KL2, which were totally revealed in about 36% of the isolates from NCBI WGS database [21]. Interestingly, CriePir298 had a recently described KL116 type [22], which is rather rare [21]. The most frequent OCL was OCL1, which was also the most common type in NCBI WGS database [21].…”

Section: Isolate Typingmentioning

confidence: 98%

Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems

et al. 2021

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Acinetobacter baumannii is an opportunistic pathogen being one of the most important causative agents of a wide range of nosocomial infections associated with multidrug resistance and high mortality rate. This study presents a multiparametric and correlation analyses of clinical multidrug-resistant A. baumannii isolates using short- and long-read whole-genome sequencing, which allowed us to reveal specific characteristics of the isolates with different CRISPR/Cas systems. We also compared antibiotic resistance and virulence gene acquisition for the groups of the isolates having functional CRISPR/Cas systems, just CRISPR arrays without cas genes, and without detectable CRISPR spacers. The data include three schemes of molecular typing, phenotypic and genotypic antibiotic resistance determination, as well as phylogenetic analysis of full-length cas gene sequences, predicted prophage sequences and CRISPR array type determination. For the first time the differences between the isolates carrying Type I-F1 and Type I-F2 CRISPR/Cas systems were investigated. A. baumannii isolates with Type I-F1 system were shown to have smaller number of reliably detected CRISPR arrays, and thus they could more easily adapt to environmental conditions through acquisition of antibiotic resistance genes, while Type I-F2 A. baumannii might have stronger “immunity” and use CRISPR/Cas system to block the dissemination of these genes. In addition, virulence factors abaI, abaR, bap and bauA were overrepresented in A. baumannii isolates lacking CRISPR/Cas system. This indicates the role of CRISPR/Cas in fighting against phage infections and preventing horizontal gene transfer. We believe that the data presented will contribute to further investigations in the field of antimicrobial resistance and CRISPR/Cas studies.

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“…To date, 128 KL gene clusters were identified in A. baumannii [ 38 ]. Therefore, the monosaccharide composition and CPS structure are highly variable in A. baumannii strains [ 37 , 39 , 40 , 41 , 42 , 43 ]. K units vary in length and may consist of two to six residues.…”

Section: A Baumannii and The Host Innate Immunmentioning

confidence: 99%

Mechanisms Protecting Acinetobacter baumannii against Multiple Stresses Triggered by the Host Immune Response, Antibiotics and Outside-Host Environment

Monem

Furmanek-Blaszk

Łupkowska

et al. 2020

IJMS

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Acinetobacter baumannii is considered one of the most persistent pathogens responsible for nosocomial infections. Due to the emergence of multidrug resistant strains, as well as high morbidity and mortality caused by this pathogen, A. baumannii was placed on the World Health Organization (WHO) drug-resistant bacteria and antimicrobial resistance research priority list. This review summarizes current studies on mechanisms that protect A. baumannii against multiple stresses caused by the host immune response, outside host environment, and antibiotic treatment. We particularly focus on the ability of A. baumannii to survive long-term desiccation on abiotic surfaces and the population heterogeneity in A. baumannii biofilms. Insight into these protective mechanisms may provide clues for the development of new strategies to fight multidrug resistant strains of A. baumannii.

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Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures

Cited by 30 publications

References 17 publications

Specific Interaction of NovelFriunavirusPhages Encoding Tailspike Depolymerases with Corresponding Acinetobacter baumannii Capsular Types

Specific Interaction of NovelFriunavirusPhages Encoding Tailspike Depolymerases with Corresponding Acinetobacter baumannii Capsular Types

Genomic and Phenotypic Analysis of Multidrug-Resistant Acinetobacter baumannii Clinical Isolates Carrying Different Types of CRISPR/Cas Systems

Mechanisms Protecting Acinetobacter baumannii against Multiple Stresses Triggered by the Host Immune Response, Antibiotics and Outside-Host Environment

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