Acetylcholinesterase immobilised eggshell membrane-based optical biosensor for organophosphate detection

Kaur, Navpreet; Thakur, Himkusha; Pathak, Smriti; Prabhakar, Nirmal

doi:10.1080/03067319.2015.1085526

Cited by 13 publications

(3 citation statements)

References 38 publications

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“…As expected, the main components of the ESM are carbon, hydrogen, nitrogen, oxygen, and sulfur. As has been shown elsewhere [31,32], the Fourier Transform Infrared (FTIR) spectrum of ESM (Figure 3) shows, in the region of higher wavenumbers, an intensive peak at 3271 cm −1 , which corresponds to the stretching mode of O-H and N-H groups. Peaks at 2923 and 2102 cm −1 correspond to the asymmetric stretching vibrations of the C-H bonds present in =C-H and =C-H 2 groups, while in the region with lower wavenumbers, the peaks at 1628 cm −1 , 1531 cm −1 , and 1446 cm −1 represent the C=O stretching, N-H bending amide absorption bonds, and CH 2 scissoring bonds, respectively.…”

Section: Resultssupporting

confidence: 65%

Polycarboxylated Eggshell Membrane Scaffold as Template for Calcium Carbonate Mineralization

et al. 2020

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Biomineralization is a process in which specialized cells secrete and deliver inorganic ions into confined spaces limited by organic matrices or scaffolds. Chicken eggshell is the fastest biomineralization system on earth, and therefore, it is a good experimental model for the study of biomineralization. Eggshell mineralization starts on specialized dispersed sites of the soft fibrillar eggshell membranes referred to as negatively charged keratan sulfate mammillae. However, the rest of the fibrillar eggshell membranes never mineralizes, although 21% of their amino acids are acidic. We hypothesized that, relative to the mammillae, the negatively charged amino acids of the fibrillar eggshell membranes are not competitive enough to promote calcite nucleation and growth. To test this hypothesis, we experimentally increased the number of negatively charged carboxylate groups on the eggshell membrane fibers and compared it with in vitro calcite deposition of isolated intact eggshell membranes. We conclude that the addition of poly-carboxylated groups onto eggshell membranes increases the number of surface nucleation sites but not the crystal size.

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Section: Resultssupporting

confidence: 65%

Polycarboxylated Eggshell Membrane Scaffold as Template for Calcium Carbonate Mineralization

et al. 2020

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“…The detection limit for paraoxon was 10 ng/ml. Kaur et al developed an AChE biosensor for the detection of malathion [59].…”

Section: Ellmanmentioning

confidence: 99%

Recent Developments on Colorimetric and Dual Colorimetric/Fluorimetric Enzymatic Biosensors for the Detection of Pesticides in Food

Gaudin¹

2023

Preprint

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Pesticides are chemical products are used to protect crops to prevent or eliminate undesirable organisms. Pesticides can contaminate continental waters, air, soil and food products. The presence of pesticide residues must therefore be monitored in water and food using sensitive, reliable and broad-spectrum analytical methods, as there is a huge variety of pesticides. Biosensors are one of the screening methods with great potential for detecting residues below the maximum residue limits. The different sensing principles of enzymatic biosensors for pesticide analysis will be described. This review highlights the great potential of colorimetric, enzymatic biosensors for detecting pesticide residues in water and food. Moreover the development of dual colorimetric and fluorimetric sensors during the past years is presented. These methods are most often based on the enzyme acetylcholinesterase, whose activity is inhibited by pesticides. The different approaches used in the development of these biosensors will be detailed, based on different chemical substrates, fluorophores and nanomaterials. The advantages and disadvantages of each approach will be discussed. Finally, the development prospects for improving these biosensors will be presented.

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“…In civilian life, similar compounds are widely used in agriculture as herbicides/pesticides, despite environmental evidence that these chemicals have been transported through our water systems and into the food chain [5,6], with adverse health effects most keenly felt by our minor and prenatal subpopulations [7][8][9][10][11][12]. Efforts to detect and remove these chemicals continue, but current detection methods that are both sensitive and selective remain elusive [13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Magnetic and Impedance Analysis of Fe2O3 Nanoparticles for Chemical Warfare Agent Sensing Applications

Soliz,

Ranjit,

Phillips

et al. 2023

Magnetochemistry

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A dire need for real-time detection of toxic chemical compounds exists in both civilian and military spheres. In this paper, we demonstrate that inexpensive, commercially available Fe2O3 nanoparticles are capable of selective sensing of chemical warfare agents (CWAs) using frequency-dependent impedance spectroscopy, with additional potential as an orthogonal magnetic sensor. X-ray magnetic circular dichroism analysis shows that Fe2O3 nanoparticles possess moderately lowered moment upon exposure to 2-chloroethyl ethyl sulfide (2-CEES) and diisopropyl methylphosphonate (DIMP) and significantly lowered moment upon exposure to dimethyl methylphosphonate (DMMP) and dimethyl chlorophosphate (DMCP). Associated X-ray absorption spectra confirm a redox reaction in the Fe2O3 nanoparticles due to CWA structural analog exposure, with differentiable energy-dependent features that suggest selective sensing is possible, given the correct method. Impedance spectroscopy performed on samples dosed with DMMP, DMCP, and tabun (GA, chemical warfare nerve agent) showed strong, differentiable, frequency-dependent responses. The frequency profiles provide unique “shift fingerprints” with which high specificity can be determined, even amongst similar analytes. The results suggest that frequency-dependent impedance fingerprinting using commercially available Fe2O3 nanoparticles as a sensor material is a feasible route to selective detection.

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Acetylcholinesterase immobilised eggshell membrane-based optical biosensor for organophosphate detection

Cited by 13 publications

References 38 publications

Polycarboxylated Eggshell Membrane Scaffold as Template for Calcium Carbonate Mineralization

Polycarboxylated Eggshell Membrane Scaffold as Template for Calcium Carbonate Mineralization

Recent Developments on Colorimetric and Dual Colorimetric/Fluorimetric Enzymatic Biosensors for the Detection of Pesticides in Food

Magnetic and Impedance Analysis of Fe2O3 Nanoparticles for Chemical Warfare Agent Sensing Applications

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