Abstract:1. Paired iris smooth muscles from rabbits were incubated for 30min at 37°C in an iso-osmotic salt medium containing glucose, inositol, cytidine and [32P]phosphate. 2. One of the pair was then incubated at 37'C for 10min in unlabelled medium containing lOmM-2-deoxyglucose and the other was incubated in the presence of acetylcholine plus eserine (0.05mM each). 2-Deoxyglucose, which was included in the incubation medium to minimize the biosynthesis of triphosphoinositide from ATP and diphosphoinositide, decrease… Show more
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