Acetylcholine Content of Discrete Areas of the Brain Obtained by a Near‐freezing Method*

Takahashi, Ryo; Aprison, M. H.

doi:10.1111/j.1471-4159.1964.tb06740.x

Cited by 187 publications

(35 citation statements)

References 5 publications

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“…Striatal ACh tissue content was measured among the animals in the second microdialysis experiment, in the contralateral, non-probe-lesioned striatum, using a near-freezing procedure (Takahashi and Aprison, 1964). Animals were sacrificed immediately after the experiment.…”

Section: Striatal Ach Tissue Contentmentioning

confidence: 99%

Dietary supplementation with uridine-5′-monophosphate (UMP), a membrane phosphatide precursor, increases acetylcholine level and release in striatum of aged rat

2007

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The biosynthesis of brain membrane phosphatides, e.g., phosphatidylcholine (PtdCho), may utilize three circulating compounds: choline, uridine (a precursor for UTP, CTP, and CDP-choline), and a PUFA (e.g., docosahexaenoic acid); moreover oral administration of the uridine source uridine-5′-monophosphate (UMP) can significantly increase levels of the phosphatides throughout the rodent brain. Since PtdCho can provide choline for acetylcholine (ACh) synthesis, we determined whether UMP administration also affects ACh levels in striatum and striatal extracellular fluid, in aged and young rats. Among aged animals consuming a UMP-containing diet (2.5%, w/w) for 1 or 6 weeks, baseline ACh levels in striatal dialysates rose from 73 fmol/min to 148 or 197 fmol/min (P<0.05). Consuming a lower dose (0.5%) for 1 week produced a smaller but still significant increase (from 75 to 92 fmol/min, P<0.05), and elevated striatal Ach content (by 16%; P<0.05). Dietary UMP (0.5%, 1 week) also amplified the increase in ACh caused by giving atropine (10 μM in the aCSF); atropine alone increased ACh concentrations from 81 to 386 fmol/min in control rats and from 137 to 680 fmol/min in those consuming UMP (P<0.05). Young rats eating the UMP-containing diet exhibited similar increases in basal ECF ACh (from 105 to 118 fmol/min) and in the increase produced by atropine (from 489 to 560 fmol/min; P<0.05). These data suggest that giving a uridine source may enhance some cholinergic functions, perhaps by increasing brain phosphatide levels. Scope: Section 3 (Neurophysiology, Neuropharmacology and other forms of Intercellular Communication)

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Section: Striatal Ach Tissue Contentmentioning

confidence: 99%

Dietary supplementation with uridine-5′-monophosphate (UMP), a membrane phosphatide precursor, increases acetylcholine level and release in striatum of aged rat

2007

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“…Therefore, in present experiments the animals were sharply immobilized by freezing method (dipping their noses into liquid nitrogen for 3-4 sec) and decapitated [35]. After such procedure the full absence of somatic reflexes on extra stimuli was recorded.…”

Section: Tissue Preparationmentioning

confidence: 99%

Age-Dependent Brain Tissue Hydration, Ca Exchange and their Dose- Dependent Ouabain Sensitivity

Ayrapetyan¹

2012

JBB

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“…Therefore, in present experiments animals were sharply immobilized by freezing method (dipping their noses into liquid nitrogen for 3-4 sec) and decapitated [20]. After such a procedure the full absence of somatic reflexes on extra stimuli was recorded.…”

Section: Tissue Preparationmentioning

confidence: 99%

Non-thermal microwave radiation-induced brain tissue dehydration as a potential factor for brain functional impairment

Nikoghosyan

Heqimyan

Ayrapetyan

2016

IJBAS

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Based on our previous finding that metabolically controlled cell hydration is sensitive to the changes of physicochemical properties of cell aqua medium, which take place upon the effect of weak physical signals, including microwaves with non-thermal intensity (NT MW), it has been hypothesized that cell aqua medium serves as a primary target for NT MW effect on brain tissue. To elucidate the nature of the metabolic pathway through which the effect of NT MW-induced changes of physicochemical properties of cell aqua medium modulate cell hydration, the effects of intraperitoneal (IP) injection of PS treated by NT MW on brain tissue hydration, 45 Ca 2+ uptake, ] i), it is considered as a potential factor leading to the brain functional impairment, especially when brain metabolic activity is depressed (e.g. during aging).

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Acetylcholine Content of Discrete Areas of the Brain Obtained by a Near‐freezing Method*

Cited by 187 publications

References 5 publications

Dietary supplementation with uridine-5′-monophosphate (UMP), a membrane phosphatide precursor, increases acetylcholine level and release in striatum of aged rat

Dietary supplementation with uridine-5′-monophosphate (UMP), a membrane phosphatide precursor, increases acetylcholine level and release in striatum of aged rat

Age-Dependent Brain Tissue Hydration, Ca Exchange and their Dose- Dependent Ouabain Sensitivity

Non-thermal microwave radiation-induced brain tissue dehydration as a potential factor for brain functional impairment

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