Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence

Pan, Tianyun; Yao, Qian; Tian, Li; Zhang, Zikai; He, Yucang; Wang, Jingping; Li, Liqun; Hu, Yun; Lin, Min-Sheng

doi:10.1007/s10616-021-00514-y

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…According to a previous method, 15 EdU assay was conducted to detect the activity of DNA replication which reflected the change of proliferation ability of HDFs. Results were expressed as the percentage of EdU‐positive cells.…”

Section: Methodsmentioning

confidence: 99%

Curcumin alleviates hypertrophic scarring by inhibiting fibroblast activation and regulating tissue inflammation

Fei

Qian

Pan

et al. 2023

J of Cosmetic Dermatology

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BackgroundHypertrophic scar (HS) that can lead to defects in appearance and function is often characterized by uncontrolled fibroblast proliferation and excessive inflammation. Curcumin has been shown to have anti‐inflammatory and anti‐oxidative effects and to play an anti‐fibrotic role by interfering transforming growth factor‐β1 (TGF‐β1)/Smads signaling pathways.AimTo study the effect and mechanism of curcumin on HS from the perspective of fibroblast activity and inflammation regulation.MethodsCell proliferation, migration and the expression of α‐smooth muscle actin (α‐SMA) of TGF‐β1‐induced human dermal fibroblasts (HDFs) treated by curcumin were evaluated using Cell Counting Kit‐8 assay, 5‐ethynyl‐2′‐deoxyuridine staining, Transwell assay, Western blotting and immunofluorescence, respectively. The expression of TGF‐β1/Smad3 pathway‐related molecules (TGF‐β1, TGFβ‐R1/2, p‐Smad3, Smad4) was detected by Western blotting. In a rabbit ear model, hematoxylin and eosin and Masson's staining were conducted to assess scar elevation and collagen deposition, and immunohistochemistry was performed to detect the activation of fibroblasts and infiltration of inflammatory cells.ResultsCurcumin inhibited proliferation, migration and α‐SMA expression of HDFs in a dose‐dependent manner. Curcumin (25 μm mol/L) did not regulate the expression of endogenous TGF‐β1, but suppressed Smad3 phosphorylation and nuclear translocation, leading to lower α‐SMA expression. Curcumin also reduced hypertrophic scarring of rabbit ear, accompanied by the inhibited TGF‐β1/Smad3 pathway, inflammatory infiltration and M2 macrophage polarization.ConclusionCurcumin plays an anti‐scar role through regulating fibroblast activation and tissue inflammation. Our findings provide scientific reference for the clinical use of curcumin in the treatment of HS.

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Section: Methodsmentioning

confidence: 99%

Curcumin alleviates hypertrophic scarring by inhibiting fibroblast activation and regulating tissue inflammation

Fei

Qian

Pan

et al. 2023

J of Cosmetic Dermatology

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“…Takahashi et al [89] found that although serum deprivation inhibited the viability of MSCs, AD-MSCs were more tolerant to hypoxia, oxidative stress, and serum-free condition than BMSCs. In addition, hypoxia [112,113] or glucose deprivation [114][115][116] under serum deprivation conditions can significantly reduce the viability and proliferation of MSCs and increase the production of reactive oxygen species and apoptosis. Berberine protects MSCs from autophagy and apoptosis through the AMP-activated protein kinase (AMPK) signaling pathway [113,114].…”

Section: Nutrient Deficiency Ivd Cells Are Supplied With Essentialmentioning

confidence: 99%

“…Berberine protects MSCs from autophagy and apoptosis through the AMP-activated protein kinase (AMPK) signaling pathway [ 113 , 114 ]. Similarly, an appropriate concentration of acetyl-L-carnitine (ALC) can protect MSCs from nutrient deprivation-induced injury by enhancing the expression of survival signals and reducing the expression of death signals [ 115 , 116 ]. Some studies have reported the impact of hypoxia and serum deprivation on rare type MSCs.…”

Section: Influence Of the Ivd Microenvironmental Factors On Mscsmentioning

confidence: 99%

The Influence of Intervertebral Disc Microenvironment on the Biological Behavior of Engrafted Mesenchymal Stem Cells

Zhang

Sun

et al. 2022

Stem Cells International

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Intervertebral disc degeneration is the main cause of low back pain. Traditional treatment methods cannot repair degenerated intervertebral disc tissue. The emergence of stem cell therapy makes it possible to regenerate and repair degenerated intervertebral disc tissue. At present, mesenchymal stem cells are the most studied, and different types of mesenchymal stem cells have their own characteristics. However, due to the harsh and complex internal microenvironment of the intervertebral disc, it will affect the biological behaviors of the implanted mesenchymal stem cells, such as viability, proliferation, migration, and chondrogenic differentiation, thereby affecting the therapeutic effect. This review is aimed at summarizing the influence of each intervertebral disc microenvironmental factor on the biological behavior of mesenchymal stem cells, so as to provide new ideas for using tissue engineering technology to assist stem cells to overcome the influence of the microenvironment in the future.

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“…Numerous biological functions of ALC are induced by the metabolic effects of its acetyl and carnitine components, which are essential for a variety of intracellular and metabolic processes, including fatty acid transport into mitochondria, the stability of cell membranes, and a decrease in serum lipid concentrations, and its acetyl group can sustain cetyl-CoA levels [ 27 , 28 ]. ALC participates in the translocation of acetyl units across the mitochondrial membranes in both anabolic and catabolic pathways [ 29 ], and furthermore it is a common free radical scavenger and a regulator of energy metabolism and metabolic processes [ 30 ]. ALC also exhibits anti-apoptotic and anti-inflammatory properties [ 31 , 32 , 33 , 34 , 35 ] in addition to its stabilizing action on the mitochondrial membrane [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

The Potential Inhibitory Role of Acetyl-L-Carnitine on Proliferation, Migration, and Gene Expression in HepG2 and HT29 Human Adenocarcinoma Cell Lines

Albogami

2023

CIMB

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Malignancies of the liver and colon are the most prevalent forms of digestive system cancer globally. Chemotherapy, one of the most significant treatments, has severe side effects. Chemoprevention using natural or synthetic medications can potentially reduce cancer severity. Acetyl-L-carnitine (ALC) is an acetylated derivative of carnitine essential for intermediate metabolism in most tissues. This study aimed to investigate the effects of ALC on the proliferation, migration, and gene expression of human liver (HepG2) and colorectal (HT29) adenocarcinoma cell lines. The cell viability and half maximal inhibitory concentration of both cancer cell lines were determined using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Wound healing after treatment was assessed using a migration assay. Morphological changes were imaged using brightfield and fluorescence microscopy. Post treatment, apoptotic DNA was detected using a DNA fragmentation assay. The relative mRNA expressions of matrix metallopeptidase 9 (MMP9) and vascular endothelial growth factor (VEGF) were evaluated using RT-PCR. The results showed that ALC treatment affects the wound-healing ability of HepG2 and HT29 cell lines. Changes in nuclear morphology were detected under fluorescent microscopy. ALC also downregulates the expression levels of MMP9 and VEGF in HepG2 and HT29 cell lines. Our results indicate that the anticancer action of ALC is likely mediated by a decrease in adhesion, migration, and invasion.

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Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence

Cited by 5 publications

References 41 publications

Curcumin alleviates hypertrophic scarring by inhibiting fibroblast activation and regulating tissue inflammation

Curcumin alleviates hypertrophic scarring by inhibiting fibroblast activation and regulating tissue inflammation

The Influence of Intervertebral Disc Microenvironment on the Biological Behavior of Engrafted Mesenchymal Stem Cells

The Potential Inhibitory Role of Acetyl-L-Carnitine on Proliferation, Migration, and Gene Expression in HepG2 and HT29 Human Adenocarcinoma Cell Lines

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