Acellular dermal matrix decorated with collagen-affinity peptide accelerate diabetic wound healing through sustained releasing Histatin-1 mediated promotion of angiogenesis

Multifunctional sponge scaffold loaded with concentrated growth factors for promoting wound healing

“… 42 After in vivo implantation, the mechanical strength will drop so quickly that the fibroblasts will not have enough time to produce qualified extracellular matrix for the autologous tissue; if the degradation is too slow, the regeneration and restoration of tissues will be affected, triggering immune reactions such as rejection and encapsulation. 43 Therefore, studying the in vivo degradation process of collagen-based biomaterials is of great significance for improving the coordination between the degradation process and the autologous tissue formation of the receptor. In vitro degradation test results ( Figure 6 D) showed that the CADMS-G-Hep group and CADMS-G-2-Hep group still had residues after 24 h, while no visible residues were observed in the control group.…”

Engineering Hydrogels for Affinity-Based Release of Therapeutic Proteins

“…Collagen is recognized by collagenases through its collagen-binding domain (CBD), from which peptides, such as the TKKTLRT heptapeptide, have been derived to specifically bind collagen. TKKTLRT has been leveraged for recombinant expression with various proteins to govern their release from collagen hydrogel delivery vehicles. − …”

“…Fusion proteins that incorporate a therapeutic protein and a binding domain with complementary peptide or protein receptors have also enabled affinity-based release. Collagen-binding domains have been recombinantly expressed with histatin-1, FGF-2, and SDF-1α to control release from various hydrogel delivery vehicles that incorporate collagen or collagen mimetic peptides. − Other approaches have attempted to improve bioorthogonality and specificity. Mulyasasmita and co-workers developed the mixing-induced two-component hydrogel (MITCH) system that sustained the release of the QK peptide (a 15-amino acid VEGF mimetic peptide) by fusing it to proline-rich peptides that interact with WW domains immobilized in a self-assembling peptide hydrogel .…”

Green biomanufacturing in recombinant collagen biosynthesis: trends and selection in various expression systems

“…Collagen is a highly effective material for drug delivery and sustained-release purposes, as it can bind to a variety of cytokines, proteins, and drugs, and has the capability to regulate the rate and timing of drug release for optimal therapeutic effects. 141–143 Only 1 clinical trial was included in ClinicalTrials.gov. for dexamethasone.…”