Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device

Wang, Hanling; Meng, Xianzhuo; Yao, Li; Wu, Qian; Yao, Bangben; Chen, Zhaoran; Xu, Jianguo; Chen, Wei

doi:10.1016/j.fochms.2023.100180

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…It has been reported that small amplicons with fragments less than 150 bp are recommended due to their increased sensitivity (LOD) in qPCR assays compared to longer fragments exceeding 200 bp [26]. On the other hand, several studies have been published in which the LAMP assay was shown to have the same detection capability as qPCR [27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

The Utilization of the SaLux19 -Based LAMP Assay for the Rapid and Sensitive Identification of Minute Amounts of Biological Material

Ruszova,

Vanek,

Stühmer

et al. 2024

Preprint

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We investigated the development of a simple and sensitive assay for the identification of minute amounts of animal and human biological material. The suggested system uses isothermal amplification of DNA in a rapid assay format, which returns the results in as little as 40 minutes from sampling. The assay used for the proof of concept employs the novel handheld SaLux19 device (Max-Planck Innovation prototype, Germany). This well-established infield detection system could also be utilized for forensic scenarios. The results presented here demonstrated that the assay is sufficiently specific and sensitive and can detect the presence of Sus scrofa nucleic acids.

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Section: Discussionmentioning

confidence: 99%

The Utilization of the SaLux19 -Based LAMP Assay for the Rapid and Sensitive Identification of Minute Amounts of Biological Material

Ruszova,

Vanek,

Stühmer

et al. 2024

Preprint

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“…Figure 2 shows a scheme of the ICT for amplicon detection. Thus, visualization of PCR products using ICT made it possible to increase sensitivity by 10 times in comparison with gel electrophoresis and significantly reduce duration of the analysis [34][35][36].…”

Section: Methods Based On Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Molecular genetic methods for identifying raw materials in meat products: Diversity, opportunities and prospects

Safenkova,

Vostrikova,

Taranova

et al. 2024

Teor. prakt. pererab. mâsa (Print)

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In the current economic situation, after easing the Covid pandemic restrictions, almost all laboratories, which are focused on evaluation of the conformity of food products, have faced issues in supplying for their laboratories. In this regard, in the last years many laboratories have been forced to validate new approaches and introduce new methods for assessing conformity of the food products. Very often it is not possible to use only one method to resolve the issue of the food product ingredients, especially for the purpose of traceability of their names and the used raw materials, listed on the label. Survey of the raw food materials to determine whether they correspond to the type name is a simpler task, in contrast to survey of the multicomponent food product. Many researchers have to estimate the opportunities and feasibility of application of various methodologies in their workplaces. Therefore, this review is relevant for the researchers in this field, as it focuses on aspects and special features of similar methodologies. The prospect of molecular genetic methods for identification of the raw materials used for manufacturing of meat products is presented below. This review also represents characteristics of methods for identification of the sources of raw materials used for the manufacturing of the meat products, based on the recognition of species-specific sections within the nucleic acids structures. The variety of methods (hybridization methods, polymerase chain reaction, different types of isothermal amplifications, methods using CRISPR/Cas systems), the principles of their implementation, and achieved analytical characteristics are considered. The capacities and competitive potential of various methods are discussed, as well as approaches being developed to overcome the existing limitations.

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The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen

Ruszova,

Vanek,

Stühmer

et al. 2024

Life

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Our research has developed a highly sensitive and simple assay to detect small amounts of animal and human biological material in less than 40 min. The handheld SaLux19 device developed at the Max Planck Institute of Experimental Medicine in Göttingen, Germany, was used to validate our concept. The proposed system uses isothermal amplification of DNA in a rapid assay format. Our results show that the assay can detect Sus scrofa nucleic acids with very high sensitivity and specificity. This detection system has potential for forensic scenarios.

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Accurate molecular identification of different meat adulterations without carryover contaminations on a microarray chip PCR-directed microfluidic lateral flow strip device

Cited by 3 publications

References 32 publications

The Utilization of the SaLux19 -Based LAMP Assay for the Rapid and Sensitive Identification of Minute Amounts of Biological Material

The Utilization of the SaLux19 -Based LAMP Assay for the Rapid and Sensitive Identification of Minute Amounts of Biological Material

Molecular genetic methods for identifying raw materials in meat products: Diversity, opportunities and prospects

The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen

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