2017
DOI: 10.1590/s1519-99402017000300004 View full text |Buy / Rent full text
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Abstract: SUMMARY The cowside determination of blood β-hydroxybutyrate (BHB) is an important tool for diagnosing subclinical ketosis in dairy cattle. Portable methods to measure BHB have been introduced in the past years. This study evaluated the accuracy of two hand-held electronic devices for blood determination of BHB in dairy cows at early postpartum. A total of 98 blood samples were collected from dairy cows in the first month of lactation and tested with FreeStyle Optium (FSO, Abbott) and KetoVet (KVE, TaiDoc) por… Show more

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“…The BHB concentration in blood was measured immediately after blood sampling, using a handheld device (FreeStyle Optium Blood Glucose and Ketone Monitoring System, Abbott Diabetes Care Ltd., Maidenhead, Berkshire, UK), as per the manufacturer's instructions. This handheld device has been recently validated previously for use on dairy cows and is known to provide very accurate results (Fiorentin et al, 2017). All blood samples were collected at approximately the same time of the day (0700 h; before the AM milking and before a fresh allocation of pasture and supplementary feed were offered), between June and October 2016.…”
Section: Animals and Reference Analysismentioning
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“…The BHB concentration in blood was measured immediately after blood sampling, using a handheld device (FreeStyle Optium Blood Glucose and Ketone Monitoring System, Abbott Diabetes Care Ltd., Maidenhead, Berkshire, UK), as per the manufacturer's instructions. This handheld device has been recently validated previously for use on dairy cows and is known to provide very accurate results (Fiorentin et al, 2017). All blood samples were collected at approximately the same time of the day (0700 h; before the AM milking and before a fresh allocation of pasture and supplementary feed were offered), between June and October 2016.…”
Section: Animals and Reference Analysismentioning
“…Glucose (catalog n. 113; wave-length 505 nm), triglycerides (catalog n. 87; wave-length 505 nm), and total cholesterol (catalog n. 76; wave-length 500 nm) were assayed colorimetrically (Kasuaki IL-120 UV-Vis Spectrophotometer) using Labtest kits ( Labtest, 2022 ). β-hydroxybutyrate was quantified using a FreeStyle Optium (FSO, Abbott) portable device, following validation for non-human vertebrates ( Fiorentin et al, 2017 ; Ribeiro et al, 2021 ). All assays were carried out strictly following the manufacturer’s instructions and with previously sterilized material to avoid contamination.…”
Section: Methodsmentioning
“…The β-hydroxybutyrate concentrations were ascertained by using a cytometer of human use (FreeStyle ® , Optium Neo™, Abbott, Oxfordshire, United Kingdom). 50 μl of blood was placed on the cytometer strip and the value displayed on the screen (mmol/l; Fiorentin et al, 2017). Similarly, concentrations of glucose were also determined with a glucometer of human use (OneTouch ® , UltraMini™, LifeScan Inc, Milipitas, California, United States); 50 μl of blood were placed on the glucometer strip and its value (mg/dl) was recorded on the screen (Castillo-Valeriano et al, 2018).…”
Section: Methodsmentioning