Accuracy of follicle count and ovulation confirmation using magnetic resonance imaging in microminipigs with normal estrus cycles

Takasu, Masaki; Baba, Ryoko; Owada, Satsuki; Nakamura, Kotono; Almunia, Julio; Nishii, Noriko; Kitagawa, Hitoshi

doi:10.1292/jvms.17-0417

Cited by 2 publications

(2 citation statements)

References 6 publications

(6 reference statements)

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“…Estrus confirmation and MRI were performed again on the next day, and artificial insemination was repeated if follicles remained. The pregnancy date was set as the day that ovulation was confirmed on the basis of the disappearance of previously existing follicles on MRI [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

Response to estrus induction with abortion treatment in microminipigs on different days after insemination

Takasu

Maeda

Almunia

et al. 2018

Journal of Reproduction and Development

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In microminipigs, estrus induction with abortion treatment, which is typically performed between 25 and 40 days after mating, is not always successful. Thus, the authors hypothesized that it may be more difficult to induce estrus by treating microminipigs approximately 40 days after mating. Accordingly, in this study, estrus induction was performed with abortion treatment in four microminipigs as follows: 0.3 mg of cloprostenol, a prostaglandin F2-alpha analog, was administered (day 0); after 24 h, 0.15 mg of cloprostenol and 250 IU of equine chorionic gonadotrophin were administered intramuscularly and simultaneously (day 1); after 96 h, 120 IU of human chorionic gonadotropin was injected intramuscularly (day 4). These treatments were compared at two different stages of pregnancy: early treatment (26.5 ± 0.7 days) and late treatment (38.3 ± 0.8 days). In the early treatment, all four microminipigs exhibited estrus on day 5, whereas in the late treatment, estrus was observed clearly in only two pigs on day 6 and slightly in 1 pig on day 10, whereas it was unclear in 1 pig. These results suggest that it is difficult to induce estrus with abortion treatment in microminipigs at approximately 40 days after mating.

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Section: Methodsmentioning

confidence: 99%

Response to estrus induction with abortion treatment in microminipigs on different days after insemination

Takasu

Maeda

Almunia

et al. 2018

Journal of Reproduction and Development

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“…12,13 In addition, microminipigs have considerable potential, particularly for long-term studies, because of their manageable size, reaching approximately 10 kg at sexual maturity and up to 20 to 30 kg at adulthood. 14 –16…”

Section: Introductionmentioning

confidence: 99%

Spontaneous Age-Related Histopathological Changes in Microminipigs

Kangawa

Nishimura²,

Nishimura³

et al. 2019

Toxicol Pathol

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Microminipigs have become an attractive animal model for toxicology and pharmacology studies and for human disease models, owing to their manageable size. Although there are numerous reports of spontaneous age-related lesions in mice, rats, dogs, and monkeys, those in minipigs are scarce. In the present study, spontaneous age-related histopathological changes were investigated using 37 microminipigs (20 males and 17 females) that were 6 months to 10 years of age. Abnormal deposits of materials were evident in several animals from 6 years of age, and these deposits included amyloid in the renal medulla, thyroid gland, and adrenal gland, hyaline droplets in glomeruli, and fibrillar inclusions in neurons. Arterial sclerosing changes (intimal thickening, intimal proliferation, and medial mineralization) and proliferative lesions (hyperplasia of hepatocytes, follicular cells, Leydig cells, and uterine endometrial glands) were present at 4 years of age and beyond. Renal adenoma, uterine leiomyoma, and Leydig cell tumor were observed in several microminipigs. Moreover, glomerulosclerosis, renal interstitial fibrosis, thymic involution, and adrenocortical cell vacuolation were common in aging microminipigs. Since knowledge of age-related changes is helpful for pathologists, the basic information obtained in this study will be a useful reference for all future toxicity evaluations in microminipigs.

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Accuracy of follicle count and ovulation confirmation using magnetic resonance imaging in microminipigs with normal estrus cycles

Cited by 2 publications

References 6 publications

Response to estrus induction with abortion treatment in microminipigs on different days after insemination

Response to estrus induction with abortion treatment in microminipigs on different days after insemination

Spontaneous Age-Related Histopathological Changes in Microminipigs

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