Accumulation of trehalose in Saccharomyces cerevisiae growing on maltose is dependent on the TPS1 gene encoding the UDPglucose‐linked trehalose synthase

Abstract

When yeast strains were cultivated on maltose, the synthesis of trehalose already started in the exponential phase of growth, well before exhaustion of the sugar from the medium. This active pattern of trehalose accumulation was also observed in a maltose constitutive mutant strain growing on &cose. However, this accumulation was completely prevented by deletion of the TPSI gene coding for the catalytic subunit of the UDPgluccse-linked trehalose-6-phosphate synthase, indicating that no alternative pathway for trehalose synthesis exists in yeast. The active pattern of trehalose accumulation seems to be consistent with the finding that trehalose-6-phosphate synthase is more active in strains growing on maltose than on glucose.

Disruption of the Candida albicans TPS1 Gene Encoding Trehalose-6-Phosphate Synthase Impairs Formation of Hyphae and Decreases Infectivity

“…Our results also show that in C. albicans there is only one, or at least a major, trehalose-6-P synthase implicated in the synthesis of trehalose. This is also the situation in S. cerevisiae (40), although the existence of a minor trehalose-6-P synthase activity that uses ADP-glucose as a cosubstrate has been reported (15,39). The situation contrasts with that found in Aspergillus niger, where two trehalose-6-P synthases encoded by genes tpsA and tpsB have been detected.…”

Disruption of the Candida albicans TPS1 Gene Encoding Trehalose-6-Phosphate Synthase Impairs Formation of Hyphae and Decreases Infectivity

“…Our results also show that in C. albicans there is only one, or at least a major, trehalose-6-P synthase implicated in the synthesis of trehalose. This is also the situation in S. cerevisiae (40), although the existence of a minor trehalose-6-P synthase activity that uses ADP-glucose as a cosubstrate has been reported (15,39). The situation contrasts with that found in Aspergillus niger, where two trehalose-6-P synthases encoded by genes tpsA and tpsB have been detected.…”

MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

“…Previous studies have reported that the MAL gene has a positive effect on the activity of Tps1, a trehalose-6-phosphate synthase that synthesizes trehalose under stress conditions [ 12 ]. We first tested if the Tps1 activity is affected by MAL62 overexpression.…”

The Relationship Between Wort Sugar Concentration and Yeast Carbon Partitioning During Brewing Fermentations

“…Trehalose is widely accepted as an important stress protectant in yeast cells, conferring stability to the plasma membrane (Neves et al, 1991, Mansure et al, 1994, Petit & Francois, 1994, Plourde-Owobi et al, 2000, Jules et al, 2004, and glycogen is regarded as a major storage carbohydrate in yeast, serving as an energy source for maintaining cellular functions. In this study, a comparatively minor proportion of carbon was directed to trehalose and glycogen synthesis, representing approximately 0.1-0.2 % and 0.2-0.3 % of the total carbon input for higher and lower pitching rates respectively.…”

Disruption of the Candida albicans TPS1 Gene Encoding Trehalose-6-Phosphate Synthase Impairs Formation of Hyphae and Decreases Infectivity

“…Our results also show that in C. albicans there is only one, or at least a major, trehalose-6-P synthase implicated in the synthesis of trehalose. This is also the situation in S. cerevisiae (40), although the existence of a minor trehalose-6-P synthase activity that uses ADP-glucose as a cosubstrate has been reported (15,39). The situation contrasts with that found in Aspergillus niger, where two trehalose-6-P synthases encoded by genes tpsA and tpsB have been detected.…”

MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

“…Previous studies have reported that the MAL gene has a positive effect on the activity of Tps1, a trehalose-6-phosphate synthase that synthesizes trehalose under stress conditions [ 12 ]. We first tested if the Tps1 activity is affected by MAL62 overexpression.…”

The Relationship Between Wort Sugar Concentration and Yeast Carbon Partitioning During Brewing Fermentations

“…Trehalose is widely accepted as an important stress protectant in yeast cells, conferring stability to the plasma membrane (Neves et al, 1991, Mansure et al, 1994, Petit & Francois, 1994, Plourde-Owobi et al, 2000, Jules et al, 2004, and glycogen is regarded as a major storage carbohydrate in yeast, serving as an energy source for maintaining cellular functions. In this study, a comparatively minor proportion of carbon was directed to trehalose and glycogen synthesis, representing approximately 0.1-0.2 % and 0.2-0.3 % of the total carbon input for higher and lower pitching rates respectively.…”

Disruption of the Candida albicans TPS1 Gene Encoding Trehalose-6-Phosphate Synthase Impairs Formation of Hyphae and Decreases Infectivity

“…Our results also show that in C. albicans there is only one, or at least a major, trehalose-6-P synthase implicated in the synthesis of trehalose. This is also the situation in S. cerevisiae (40), although the existence of a minor trehalose-6-P synthase activity that uses ADP-glucose as a cosubstrate has been reported (15,39). The situation contrasts with that found in Aspergillus niger, where two trehalose-6-P synthases encoded by genes tpsA and tpsB have been detected.…”

MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

“…Previous studies have reported that the MAL gene has a positive effect on the activity of Tps1, a trehalose-6-phosphate synthase that synthesizes trehalose under stress conditions [ 12 ]. We first tested if the Tps1 activity is affected by MAL62 overexpression.…”

The Relationship Between Wort Sugar Concentration and Yeast Carbon Partitioning During Brewing Fermentations

“…Trehalose is widely accepted as an important stress protectant in yeast cells, conferring stability to the plasma membrane (Neves et al, 1991, Mansure et al, 1994, Petit & Francois, 1994, Plourde-Owobi et al, 2000, Jules et al, 2004, and glycogen is regarded as a major storage carbohydrate in yeast, serving as an energy source for maintaining cellular functions. In this study, a comparatively minor proportion of carbon was directed to trehalose and glycogen synthesis, representing approximately 0.1-0.2 % and 0.2-0.3 % of the total carbon input for higher and lower pitching rates respectively.…”