2016
DOI: 10.18632/aging.101142
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: Expansion of mesenchymal stromal/stem cells (MSCs) used in clinical practices may be associated with accumulation of genetic instability. Understanding temporal and mechanistic aspects of this process is important for improving stem cell therapy protocols. We used γH2AX foci as a marker of a genetic instability event and quantified it in MSCs that undergone various numbers of passage (3-22). We found that γH2AX foci numbers increased in cells of late passages, with a sharp increase at passage 16-18. By measuri… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
15
0

Year Published

2017
2017
2020
2020

Publication Types

Select...
5

Relationship

5
0

Authors

Journals

citations
Cited by 16 publications
(41 citation statements)
references
References 47 publications
(41 reference statements)
1
15
0
Order By: Relevance
“…4 ). This observation is consistent with our previous results showing that long-term culture of MSCs leads to accumulation of γH2AX foci [ 44 ]. Apparently, the increase in the γH2AX foci number at the late passages of the primary cultures of normal (non-immortalized and non-cancerous) cells may be associated with cellular senescence.…”
Section: Resultssupporting
confidence: 94%
“…Analysis of γH2AX as a biological marker of double-stranded DNA breaks has wide application in various biological studies, including for example, radiation bio-dosimetry [28], assessment of individual radio sensitivity [29], testing of medicinal and chemotherapeutic drugs [3032], assessment of the effects of genotoxic environmental agents [33], studying the mechanisms of aging [34], etc. The processes of induction and repair of DSBs in proliferating and quiescent cells are significantly different [13].…”
Section: Discussionmentioning
confidence: 99%
“…The implemented readouts may include measurement of cell viability, various indicators of DNA damage and repair markers (H2AX, 53BP1, etc.) after the exposure to IR [ 151 , 191 , 195 – 199 ]. Moreover, markers specific to the particular DNA repair mechanisms (RAD51, Ku70/80, ATM) may be of a particular interest, as numerous studies have shown higher error-proneness of non-homologous end joining compared to the homologous recombination [ 29 , 30 , 36 , 200 202 ].…”
Section: Ways To Reduce Health Risks From Space Radiationmentioning
confidence: 99%
“…The radioprotectors can elicit their action by various mechanisms and DNA protection via decreasing DNA damage is among them. Moreover, the late effects of ionizing radiation are associated with DNA damage that can be visualized by persistent DNA Damage Response (DDR) foci and might be prevented by radioprotectors [ 45 , 46 ]. Reduction of DNA damage might be reached by suppressing the formation of reactive species, detoxification of radiation-induced species, target stabilization, and enhancing the repair and recovery processes [ 22 ].…”
Section: Resultsmentioning
confidence: 99%
“…We further examined correlations between the colony phenotypes and the fraction of proliferating cells that was measured using Ki67 as a marker for cellular proliferation. Ki67 protein is present in actively proliferating cells (during G1, S, G2 and M phases of the cell cycle), while being absent in resting (G0 phase) cells [ 14 , 15 ]. The expression of Ki67 was shown to be associated with aging in that aging cells that lost their proliferative and colony forming capacity become Ki67-negative [ 16 ].…”
Section: Introductionmentioning
confidence: 99%